Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells
Arboviruses are some of the important causative agents of mosquito-mediated viral diseases. These viruses are transmitted between vector and host during the blood meal. Upon viral entry, host replication machinery is hijacked, supporting new virus particle production and thereby allowing viral survi...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/10/4/38 |
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| author | Ramesh Kumar Divya Mehta Sakshi Chaudhary Debasis Nayak Sujatha Sunil |
| author_facet | Ramesh Kumar Divya Mehta Sakshi Chaudhary Debasis Nayak Sujatha Sunil |
| author_sort | Ramesh Kumar |
| collection | DOAJ |
| description | Arboviruses are some of the important causative agents of mosquito-mediated viral diseases. These viruses are transmitted between vector and host during the blood meal. Upon viral entry, host replication machinery is hijacked, supporting new virus particle production and thereby allowing viral survival in the host. In this process, host proteins interact with viral proteins to either facilitate viral replication, or they may provide antiviral defense mechanisms. In this study, we analyzed the impact of chikungunya virus (CHIKV) infection on the global proteome of Dicer active <i>Aedes albopictus</i> cells during the early and late time points of infection. We utilized a bottom-up approach of global proteomics analysis, and we used label-free quantitative mass spectrometry to identify the global protein signatures of <i>Ae. albopictus</i> at two different time points upon CHIKV infection. The mass spectrometry data analysis of the early time point revealed that proteins belonging to pathways such as translation, RNA processing, and cellular metabolic processes were less in abundance, whereas those belonging to pathways such as cellular catabolic process and organic substance transport were significantly abundant. At later time points, proteins belonging to pathways such as cellular metabolic processes, primary metabolic process, organonitrogen compound metabolic process, and organic substance metabolic process were found to be decreased in their presence, whereas those belonging to pathways such as RNA processing, gene expression, macromolecule metabolic processing, and nitrogen compound metabolic processing were found to be abundant during CHIKV infection, indicating that modulation in gene expression favoring cell survival occurs at a later time point, suggesting a survival strategy of <i>Aedes</i> cells to counter prolonged CHIKV infection. |
| first_indexed | 2024-03-09T15:56:01Z |
| format | Article |
| id | doaj.art-fe82672c1de84f0e97543628cc708f4f |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-09T15:56:01Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-fe82672c1de84f0e97543628cc708f4f2023-11-24T17:41:40ZengMDPI AGProteomes2227-73822022-11-011043810.3390/proteomes10040038Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> CellsRamesh Kumar0Divya Mehta1Sakshi Chaudhary2Debasis Nayak3Sujatha Sunil4Vector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, IndiaVector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, IndiaVector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, IndiaDepartment of Biosciences and Biomedical Engineering, Indian Institute of Technology, Indore 453552, IndiaVector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, IndiaArboviruses are some of the important causative agents of mosquito-mediated viral diseases. These viruses are transmitted between vector and host during the blood meal. Upon viral entry, host replication machinery is hijacked, supporting new virus particle production and thereby allowing viral survival in the host. In this process, host proteins interact with viral proteins to either facilitate viral replication, or they may provide antiviral defense mechanisms. In this study, we analyzed the impact of chikungunya virus (CHIKV) infection on the global proteome of Dicer active <i>Aedes albopictus</i> cells during the early and late time points of infection. We utilized a bottom-up approach of global proteomics analysis, and we used label-free quantitative mass spectrometry to identify the global protein signatures of <i>Ae. albopictus</i> at two different time points upon CHIKV infection. The mass spectrometry data analysis of the early time point revealed that proteins belonging to pathways such as translation, RNA processing, and cellular metabolic processes were less in abundance, whereas those belonging to pathways such as cellular catabolic process and organic substance transport were significantly abundant. At later time points, proteins belonging to pathways such as cellular metabolic processes, primary metabolic process, organonitrogen compound metabolic process, and organic substance metabolic process were found to be decreased in their presence, whereas those belonging to pathways such as RNA processing, gene expression, macromolecule metabolic processing, and nitrogen compound metabolic processing were found to be abundant during CHIKV infection, indicating that modulation in gene expression favoring cell survival occurs at a later time point, suggesting a survival strategy of <i>Aedes</i> cells to counter prolonged CHIKV infection.https://www.mdpi.com/2227-7382/10/4/38chikungunya virus<i>Aedes albopictus</i>mass spectrometrypathway analysishost–pathogen interaction |
| spellingShingle | Ramesh Kumar Divya Mehta Sakshi Chaudhary Debasis Nayak Sujatha Sunil Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells Proteomes chikungunya virus <i>Aedes albopictus</i> mass spectrometry pathway analysis host–pathogen interaction |
| title | Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells |
| title_full | Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells |
| title_fullStr | Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells |
| title_full_unstemmed | Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells |
| title_short | Impact of CHIKV Replication on the Global Proteome of <i>Aedes albopictus</i> Cells |
| title_sort | impact of chikv replication on the global proteome of i aedes albopictus i cells |
| topic | chikungunya virus <i>Aedes albopictus</i> mass spectrometry pathway analysis host–pathogen interaction |
| url | https://www.mdpi.com/2227-7382/10/4/38 |
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