Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library
Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/24/7652 |
| _version_ | 1797502154803511296 |
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| author | Wei Sun Yan Zhang Zhigang Ju |
| author_facet | Wei Sun Yan Zhang Zhigang Ju |
| author_sort | Wei Sun |
| collection | DOAJ |
| description | Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins. |
| first_indexed | 2024-03-10T03:28:52Z |
| format | Article |
| id | doaj.art-fe83b85f89f647a291a7d9dafa307e1c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:28:52Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fe83b85f89f647a291a7d9dafa307e1c2023-11-23T09:47:20ZengMDPI AGMolecules1420-30492021-12-012624765210.3390/molecules26247652Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage LibraryWei Sun0Yan Zhang1Zhigang Ju2Key Laboratory of Plant Physiology and Development Regulation, School of Life Science, Guizhou Normal University, Guiyang 550001, ChinaKey Laboratory of Plant Physiology and Development Regulation, School of Life Science, Guizhou Normal University, Guiyang 550001, ChinaPharmacy School, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaMycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.https://www.mdpi.com/1420-3049/26/24/7652mycotoxinsphage displayscFvanti-idiotypic nanobodysimultaneous determination |
| spellingShingle | Wei Sun Yan Zhang Zhigang Ju Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library Molecules mycotoxins phage display scFv anti-idiotypic nanobody simultaneous determination |
| title | Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library |
| title_full | Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library |
| title_fullStr | Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library |
| title_full_unstemmed | Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library |
| title_short | Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library |
| title_sort | mimotopes for mycotoxins diagnosis based on random peptides or recombinant antibodies from phage library |
| topic | mycotoxins phage display scFv anti-idiotypic nanobody simultaneous determination |
| url | https://www.mdpi.com/1420-3049/26/24/7652 |
| work_keys_str_mv | AT weisun mimotopesformycotoxinsdiagnosisbasedonrandompeptidesorrecombinantantibodiesfromphagelibrary AT yanzhang mimotopesformycotoxinsdiagnosisbasedonrandompeptidesorrecombinantantibodiesfromphagelibrary AT zhigangju mimotopesformycotoxinsdiagnosisbasedonrandompeptidesorrecombinantantibodiesfromphagelibrary |