Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i>
Black rot, caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>), is the main disease of cruciferous vegetables. To characterize the resistance mechanism in the <i>Brassica napus</i>–<i>Xcc</i> pathosystem, <i>Xcc...
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/253 |
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| author | Md Tabibul Islam Bok-Rye Lee Van Hien La Dong-Won Bae Woo-Jin Jung Tae-Hwan Kim |
| author_facet | Md Tabibul Islam Bok-Rye Lee Van Hien La Dong-Won Bae Woo-Jin Jung Tae-Hwan Kim |
| author_sort | Md Tabibul Islam |
| collection | DOAJ |
| description | Black rot, caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>), is the main disease of cruciferous vegetables. To characterize the resistance mechanism in the <i>Brassica napus</i>–<i>Xcc</i> pathosystem, <i>Xcc</i>-responsive proteins in susceptible (cv. Mosa) and resistant (cv. Capitol) cultivars were investigated using gel-free quantitative proteomics and analysis of gene expression. This allowed us to identify 158 and 163 differentially expressed proteins following <i>Xcc</i> infection in cv. Mosa and cv. Capitol, respectively, and to classify them into five major categories including antioxidative systems, proteolysis, photosynthesis, redox, and innate immunity. All proteins involved in protein degradation such as the protease complex, proteasome subunits, and ATP-dependent Clp protease proteolytic subunits, were upregulated only in cv. Mosa, in which higher hydrogen peroxide accumulation concurred with upregulated superoxide dismutase. In cv. Capitol, photosystem II (PS II)-related proteins were downregulated (excepting PS II 22 kDa), whereas the PS I proteins, ATP synthase, and ferredoxin-NADP<sup>+</sup> reductase, were upregulated. For redox-related proteins, upregulation of thioredoxin, 2-cys peroxiredoxin, and glutathione S-transferase occurred in cv. Capitol, consistent with higher NADH-, ascorbate-, and glutathione-based reducing potential, whereas the proteins involved in the C<sub>2</sub> oxidative cycle and glycolysis were highly activated in cv. Mosa. Most innate immunity-related proteins, including zinc finger domain (ZFD)-containing protein, glycine-rich RNA-binding protein (GRP) and mitochondrial outer membrane porin, were highly enhanced in cv. Capitol, concomitant with enhanced expression of <i>ZFD</i> and <i>GRP</i> genes. Distinguishable differences in the protein profile between the two cultivars deserves higher importance for breeding programs and understanding of disease resistance in the <i>B. napus</i>–<i>Xcc</i> pathosystem. |
| first_indexed | 2024-03-09T03:32:03Z |
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| id | doaj.art-fe87401b41394afda8042dcbbdb8d526 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:32:03Z |
| publishDate | 2021-01-01 |
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| series | Microorganisms |
| spelling | doaj.art-fe87401b41394afda8042dcbbdb8d5262023-12-03T14:54:19ZengMDPI AGMicroorganisms2076-26072021-01-019225310.3390/microorganisms9020253Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i>Md Tabibul Islam0Bok-Rye Lee1Van Hien La2Dong-Won Bae3Woo-Jin Jung4Tae-Hwan Kim5Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, KoreaDepartment of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, KoreaDepartment of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, KoreaCentral Instrument Facility, Gyeongsang National University, Jinju 52828, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture (IEFA), College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, KoreaDepartment of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 61186, KoreaBlack rot, caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>), is the main disease of cruciferous vegetables. To characterize the resistance mechanism in the <i>Brassica napus</i>–<i>Xcc</i> pathosystem, <i>Xcc</i>-responsive proteins in susceptible (cv. Mosa) and resistant (cv. Capitol) cultivars were investigated using gel-free quantitative proteomics and analysis of gene expression. This allowed us to identify 158 and 163 differentially expressed proteins following <i>Xcc</i> infection in cv. Mosa and cv. Capitol, respectively, and to classify them into five major categories including antioxidative systems, proteolysis, photosynthesis, redox, and innate immunity. All proteins involved in protein degradation such as the protease complex, proteasome subunits, and ATP-dependent Clp protease proteolytic subunits, were upregulated only in cv. Mosa, in which higher hydrogen peroxide accumulation concurred with upregulated superoxide dismutase. In cv. Capitol, photosystem II (PS II)-related proteins were downregulated (excepting PS II 22 kDa), whereas the PS I proteins, ATP synthase, and ferredoxin-NADP<sup>+</sup> reductase, were upregulated. For redox-related proteins, upregulation of thioredoxin, 2-cys peroxiredoxin, and glutathione S-transferase occurred in cv. Capitol, consistent with higher NADH-, ascorbate-, and glutathione-based reducing potential, whereas the proteins involved in the C<sub>2</sub> oxidative cycle and glycolysis were highly activated in cv. Mosa. Most innate immunity-related proteins, including zinc finger domain (ZFD)-containing protein, glycine-rich RNA-binding protein (GRP) and mitochondrial outer membrane porin, were highly enhanced in cv. Capitol, concomitant with enhanced expression of <i>ZFD</i> and <i>GRP</i> genes. Distinguishable differences in the protein profile between the two cultivars deserves higher importance for breeding programs and understanding of disease resistance in the <i>B. napus</i>–<i>Xcc</i> pathosystem.https://www.mdpi.com/2076-2607/9/2/253<i>Brassica napus</i>proteolysisphotosynthesis-related proteinsredoxinsredox status<i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| spellingShingle | Md Tabibul Islam Bok-Rye Lee Van Hien La Dong-Won Bae Woo-Jin Jung Tae-Hwan Kim Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i> Microorganisms <i>Brassica napus</i> proteolysis photosynthesis-related proteins redoxins redox status <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| title | Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| title_full | Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| title_fullStr | Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| title_full_unstemmed | Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| title_short | Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant <i>Brassica napus</i> Cultivars Infected with <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| title_sort | label free quantitative proteomics analysis in susceptible and resistant i brassica napus i cultivars infected with i xanthomonas campestris i pv i campestris i |
| topic | <i>Brassica napus</i> proteolysis photosynthesis-related proteins redoxins redox status <i>Xanthomonas campestris</i> pv. <i>campestris</i> |
| url | https://www.mdpi.com/2076-2607/9/2/253 |
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