PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing
Lung-on-a-chip devices could provide new strategies for a biomimetic lung cell microenvironment and construction of lung disease models in vitro, and are expected to greatly promote the development of drug evaluation, toxicological detection, and disease model building. In this study, we developed a...
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MDPI AG
2020-11-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/12/1054 |
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| author | Wei Li Xindi Sun Bing Ji Xingyuan Yang Bingpu Zhou Zhanjun Lu Xinghua Gao |
| author_facet | Wei Li Xindi Sun Bing Ji Xingyuan Yang Bingpu Zhou Zhanjun Lu Xinghua Gao |
| author_sort | Wei Li |
| collection | DOAJ |
| description | Lung-on-a-chip devices could provide new strategies for a biomimetic lung cell microenvironment and construction of lung disease models in vitro, and are expected to greatly promote the development of drug evaluation, toxicological detection, and disease model building. In this study, we developed a novel poly (lactic-co-glycolic acid) (PLGA) nanofiber/polydimethylsiloxane (PDMS) microporous composite membrane-sandwiched lung-on-a-chip to perform anti-tumor drug testing. The composite membrane was characterized, and the results showed that it was permeable to molecules and thus could be used to study small-molecule drug diffusion. In addition, the microchip could apply perfusion fluids to simulate blood flow under extremely low fluid shear stress, and could also simulate the spherical-like shape of the alveoli by deformation of the composite membrane. Using this chip, we evaluated the anti-tumor drug efficacy of gefitinib in two kinds of non-small cell lung cancer cells, the lung adenocarcinoma NCI-H1650 cell line and the large cell lung cancer NCI-H460 cell line. We further probed the resistance of NCI-H460 cells to gefitinib under normoxic and hypoxic conditions. The established composite membrane-sandwiched lung chip can simulate more biochemical and biophysical factors in the lung physiological and pathological microenvironment, and it has important applications in the personalized treatment of lung tumors. It is expected to play a potential role in clinical diagnosis and drug screening. |
| first_indexed | 2024-03-10T14:28:43Z |
| format | Article |
| id | doaj.art-fe89efb733604096a061cc3b2e500167 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T14:28:43Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-fe89efb733604096a061cc3b2e5001672023-11-20T22:48:25ZengMDPI AGMicromachines2072-666X2020-11-011112105410.3390/mi11121054PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug TestingWei Li0Xindi Sun1Bing Ji2Xingyuan Yang3Bingpu Zhou4Zhanjun Lu5Xinghua Gao6Materials Genome Institute, Shanghai University, Shanghai 200444, ChinaMaterials Genome Institute, Shanghai University, Shanghai 200444, ChinaJoint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macau 999078, ChinaMaterials Genome Institute, Shanghai University, Shanghai 200444, ChinaJoint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macau 999078, ChinaDepartment of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, ChinaMaterials Genome Institute, Shanghai University, Shanghai 200444, ChinaLung-on-a-chip devices could provide new strategies for a biomimetic lung cell microenvironment and construction of lung disease models in vitro, and are expected to greatly promote the development of drug evaluation, toxicological detection, and disease model building. In this study, we developed a novel poly (lactic-co-glycolic acid) (PLGA) nanofiber/polydimethylsiloxane (PDMS) microporous composite membrane-sandwiched lung-on-a-chip to perform anti-tumor drug testing. The composite membrane was characterized, and the results showed that it was permeable to molecules and thus could be used to study small-molecule drug diffusion. In addition, the microchip could apply perfusion fluids to simulate blood flow under extremely low fluid shear stress, and could also simulate the spherical-like shape of the alveoli by deformation of the composite membrane. Using this chip, we evaluated the anti-tumor drug efficacy of gefitinib in two kinds of non-small cell lung cancer cells, the lung adenocarcinoma NCI-H1650 cell line and the large cell lung cancer NCI-H460 cell line. We further probed the resistance of NCI-H460 cells to gefitinib under normoxic and hypoxic conditions. The established composite membrane-sandwiched lung chip can simulate more biochemical and biophysical factors in the lung physiological and pathological microenvironment, and it has important applications in the personalized treatment of lung tumors. It is expected to play a potential role in clinical diagnosis and drug screening.https://www.mdpi.com/2072-666X/11/12/1054organ-on-a-chipmicrofluidic chipcomposite membranelung-on-a-chipdrug evaluation |
| spellingShingle | Wei Li Xindi Sun Bing Ji Xingyuan Yang Bingpu Zhou Zhanjun Lu Xinghua Gao PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing Micromachines organ-on-a-chip microfluidic chip composite membrane lung-on-a-chip drug evaluation |
| title | PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing |
| title_full | PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing |
| title_fullStr | PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing |
| title_full_unstemmed | PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing |
| title_short | PLGA Nanofiber/PDMS Microporous Composite Membrane-Sandwiched Microchip for Drug Testing |
| title_sort | plga nanofiber pdms microporous composite membrane sandwiched microchip for drug testing |
| topic | organ-on-a-chip microfluidic chip composite membrane lung-on-a-chip drug evaluation |
| url | https://www.mdpi.com/2072-666X/11/12/1054 |
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