High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells.
Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-subs...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3178539?pdf=render |
| _version_ | 1828337594666057728 |
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| author | Edgar Gutierrez Eugene Tkachenko Achim Besser Prithu Sundd Klaus Ley Gaudenz Danuser Mark H Ginsberg Alex Groisman |
| author_facet | Edgar Gutierrez Eugene Tkachenko Achim Besser Prithu Sundd Klaus Ley Gaudenz Danuser Mark H Ginsberg Alex Groisman |
| author_sort | Edgar Gutierrez |
| collection | DOAJ |
| description | Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate. |
| first_indexed | 2024-04-13T22:17:01Z |
| format | Article |
| id | doaj.art-fe8a94e219b74f8cba0599eecf4c3113 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T22:17:01Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fe8a94e219b74f8cba0599eecf4c31132022-12-22T02:27:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2380710.1371/journal.pone.0023807High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells.Edgar GutierrezEugene TkachenkoAchim BesserPrithu SunddKlaus LeyGaudenz DanuserMark H GinsbergAlex GroismanSubstrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.http://europepmc.org/articles/PMC3178539?pdf=render |
| spellingShingle | Edgar Gutierrez Eugene Tkachenko Achim Besser Prithu Sundd Klaus Ley Gaudenz Danuser Mark H Ginsberg Alex Groisman High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells. PLoS ONE |
| title | High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells. |
| title_full | High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells. |
| title_fullStr | High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells. |
| title_full_unstemmed | High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells. |
| title_short | High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells. |
| title_sort | high refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells |
| url | http://europepmc.org/articles/PMC3178539?pdf=render |
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