| Summary: | <i>Curcuma longa</i>, <i>Curcuma xanthorrhiza</i>, and <i>Curcuma manga</i> have been widely used for herbal or traditional medicine purposes. It was reported that turmeric plants provided several biological activities such as antioxidant, anti-inflammatory, hepatoprotector, cardioprotector, and anticancer activities. Authentication of the Curcuma species is important to ensure its authenticity and to avoid adulteration practices. Plants from different origins will have different metabolite compositions because metabolites are affected by soil nutrition, climate, temperature, and humidity. <sup>1</sup>H-NMR spectroscopy, principal component analysis (PCA), and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) were used for authentication of <i>C. longa</i>, <i>C. xanthorrhiza</i>, and <i>C. manga</i> from seven different origins in Indonesia. From the <sup>1</sup>H-NMR analysis it was obtained that 14 metabolites were responsible for generating classification model such as curcumin, demethoxycurcumin, alanine, methionine, threonine, lysine, alpha-glucose, beta-glucose, sucrose, alpha-fructose, beta-fructose, fumaric acid, tyrosine, and formate. Both PCA and OPLS-DA model demonstrated goodness of fit (R<sup>2</sup> value more than 0.8) and good predictivity (Q<sup>2</sup> value more than 0.45). All OPLS-DA models were validated by assessing the permutation test results with high value of original R<sup>2</sup> and Q<sup>2</sup>. It can be concluded that metabolite fingerprinting using <sup>1</sup>H-NMR spectroscopy and chemometrics provide a powerful tool for authentication of herbal and medicinal plants.
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