Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha
Introduction: Methicillin resistant Staphylococcus aureus has emerged as an important pathogen in nosocomial and community acquired infections. Accurate and rapid identification of MRSA in clinical specimens is essential for timely decision of effective antimicrobial chemotherapy. Aim: The pres...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/7278/17476_CE(RA1)_F(T)_PF1(SSAK)_PFA(AK)_PF2(AGAK).pdf |
| _version_ | 1818133404788457472 |
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| author | Rakesh Kumar Panda Ashoka Mahapatra Bandana Mallick Nirupama Chayani |
| author_facet | Rakesh Kumar Panda Ashoka Mahapatra Bandana Mallick Nirupama Chayani |
| author_sort | Rakesh Kumar Panda |
| collection | DOAJ |
| description | Introduction: Methicillin resistant Staphylococcus aureus
has emerged as an important pathogen in nosocomial and
community acquired infections. Accurate and rapid identification
of MRSA in clinical specimens is essential for timely decision of
effective antimicrobial chemotherapy.
Aim: The present study was conducted to compare efficacy
of four conventional phenotypic methods, with mec- A based
polymerase chain reaction (PCR) for MRSA identification.
Materials and Methods: Methicillin resistance was determined
in 200 S.aureus isolates by oxacillin disc diffusion, cefoxitin disc
diffusion, Oxacillin Resistance Screening Agar and E-test. The
results were compared with mec-A based PCR.
Results: Among 200 S.aureus isolates 62 (31%) were positive
for mec-A gene by PCR. Cefoxitin disc diffusion, Oxacillin
Resistance Screening Agar and E-test showed 100% specificity.
Oxacillin disc diffusion had lowest sensitivity (82.5%) and
specificity (98.5%) among all. The conventional methods
take more time than PCR for diagnosing MRSA. Linezolid,
Vancomycin & Dalfopristin were the highly sensitive drugs
against MRSA isolates.
Conclusion: Cefoxitin disc diffusion, is rapid, simple and
cheaper, hence can be used routinely as an alternative to PCR
for detection of MRSA in resource constraint laboratories. |
| first_indexed | 2024-12-11T08:52:11Z |
| format | Article |
| id | doaj.art-fe8cd54fed9f4d7b82f1bc431ed3e994 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-11T08:52:11Z |
| publishDate | 2016-02-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-fe8cd54fed9f4d7b82f1bc431ed3e9942022-12-22T01:13:59ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-02-01102DC19DC2110.7860/JCDR/2016/17476.7278Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern OdishaRakesh Kumar Panda0Ashoka Mahapatra1Bandana Mallick2Nirupama Chayani3Assistant Professor, Department of Microbiology, S.C.B Medical College, Cuttack, India.Associate Professor, Department of Microbiology, AIIMS, Bhubaneswar, India.Professor, Department of Microbiology, Kalinga Institute of Medical Sciences, Bhubaneswar, India.Professor and Head, Department of Microbiology, S.C.B Medical College, Cuttack, India.Introduction: Methicillin resistant Staphylococcus aureus has emerged as an important pathogen in nosocomial and community acquired infections. Accurate and rapid identification of MRSA in clinical specimens is essential for timely decision of effective antimicrobial chemotherapy. Aim: The present study was conducted to compare efficacy of four conventional phenotypic methods, with mec- A based polymerase chain reaction (PCR) for MRSA identification. Materials and Methods: Methicillin resistance was determined in 200 S.aureus isolates by oxacillin disc diffusion, cefoxitin disc diffusion, Oxacillin Resistance Screening Agar and E-test. The results were compared with mec-A based PCR. Results: Among 200 S.aureus isolates 62 (31%) were positive for mec-A gene by PCR. Cefoxitin disc diffusion, Oxacillin Resistance Screening Agar and E-test showed 100% specificity. Oxacillin disc diffusion had lowest sensitivity (82.5%) and specificity (98.5%) among all. The conventional methods take more time than PCR for diagnosing MRSA. Linezolid, Vancomycin & Dalfopristin were the highly sensitive drugs against MRSA isolates. Conclusion: Cefoxitin disc diffusion, is rapid, simple and cheaper, hence can be used routinely as an alternative to PCR for detection of MRSA in resource constraint laboratories.https://jcdr.net/articles/PDF/7278/17476_CE(RA1)_F(T)_PF1(SSAK)_PFA(AK)_PF2(AGAK).pdfcefoxitine-testmec amrsaorsa |
| spellingShingle | Rakesh Kumar Panda Ashoka Mahapatra Bandana Mallick Nirupama Chayani Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha Journal of Clinical and Diagnostic Research cefoxitin e-test mec a mrsa orsa |
| title | Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha |
| title_full | Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha |
| title_fullStr | Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha |
| title_full_unstemmed | Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha |
| title_short | Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha |
| title_sort | evaluation of genotypic and phenotypic methods for detection of methicillin resistant staphylococcus aureus in a tertiary care hospital of eastern odisha |
| topic | cefoxitin e-test mec a mrsa orsa |
| url | https://jcdr.net/articles/PDF/7278/17476_CE(RA1)_F(T)_PF1(SSAK)_PFA(AK)_PF2(AGAK).pdf |
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