No current evidence for widespread dosage compensation in S. cerevisiae
Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/10996 |
| _version_ | 1811201041842044928 |
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| author | Eduardo M Torres Michael Springer Angelika Amon |
| author_facet | Eduardo M Torres Michael Springer Angelika Amon |
| author_sort | Eduardo M Torres |
| collection | DOAJ |
| description | Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae. |
| first_indexed | 2024-04-12T02:15:10Z |
| format | Article |
| id | doaj.art-fe8ee90fe6a349ee9fb1af9e1cb0cc4b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:15:10Z |
| publishDate | 2016-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-fe8ee90fe6a349ee9fb1af9e1cb0cc4b2022-12-22T03:52:17ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.10996No current evidence for widespread dosage compensation in S. cerevisiaeEduardo M Torres0Michael Springer1Angelika Amon2Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Systems Biology, Harvard Medical School, Boston, United StatesDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, United States; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United StatesPrevious studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae.https://elifesciences.org/articles/10996dosage compensationaneuploidygene expression |
| spellingShingle | Eduardo M Torres Michael Springer Angelika Amon No current evidence for widespread dosage compensation in S. cerevisiae eLife dosage compensation aneuploidy gene expression |
| title | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_full | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_fullStr | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_full_unstemmed | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_short | No current evidence for widespread dosage compensation in S. cerevisiae |
| title_sort | no current evidence for widespread dosage compensation in s cerevisiae |
| topic | dosage compensation aneuploidy gene expression |
| url | https://elifesciences.org/articles/10996 |
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