Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China
The prevalence and molecular characteristics of Bartonella infections in small rodents in the Zhongtiao Mountain, China have been explored. In this study, the liver, spleen and kidney tissues of captured rodents were used for Bartonella spp. detection and identification by combination of real-time P...
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| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884503/?tool=EBI |
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| author | Juan Yu Xiong-Ying Zhang Yun-Xia Chen Hong-Bing Cheng Dong-Mei Li Hua-Xiang Rao |
| author_facet | Juan Yu Xiong-Ying Zhang Yun-Xia Chen Hong-Bing Cheng Dong-Mei Li Hua-Xiang Rao |
| author_sort | Juan Yu |
| collection | DOAJ |
| description | The prevalence and molecular characteristics of Bartonella infections in small rodents in the Zhongtiao Mountain, China have been explored. In this study, the liver, spleen and kidney tissues of captured rodents were used for Bartonella spp. detection and identification by combination of real-time PCR of transfer-mRNA (ssrA) gene and traditional PCR and sequencing of citrate synthase (gltA) gene. It was shown that 49.52% of the rodents (52/105) were positive for Bartonella spp.. The infection rate in different gender (χ2 = 0.079, P = 0.778) and tissues (χ2 = 0.233, P = 0.890) of small rodents did not have statistical difference, but that in different small rodents (Fisher’s exact test, P < 0.001) and habitats (χ2 = 5.483, P = 0.019) had statistical difference. And, the sequencing data suggests that Bartonella sequences (n = 31) were identified into three species, including 14 of B. grahamii, 3 of B. queenslandensis and 14 of unknown Bartonella species. Phylogenetic analysis showed that B. grahamii sequences were clustered with the isolates from South Korea and China, and B. queenslandensis sequences were mainly closely related to the isolates from China and Thailand. The genetic diversity analysis showed that B. grahamii and B. queenslandensis sequences exhibited noticeable intraspecies diversity. Taken together our data demonstrates the high prevalence and genetic diversity of Bartonella infections in small rodents in the Zhongtiao Mountain, especially a potential novel Bartonella specie was detected, which could benefit the prevention and control of rodent-Bartonella species in this area. |
| first_indexed | 2024-04-11T06:53:01Z |
| format | Article |
| id | doaj.art-fe8f7cac0785451ea8c50c1d6b8abed7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T06:53:01Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fe8f7cac0785451ea8c50c1d6b8abed72022-12-22T04:39:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01172Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, ChinaJuan YuXiong-Ying ZhangYun-Xia ChenHong-Bing ChengDong-Mei LiHua-Xiang RaoThe prevalence and molecular characteristics of Bartonella infections in small rodents in the Zhongtiao Mountain, China have been explored. In this study, the liver, spleen and kidney tissues of captured rodents were used for Bartonella spp. detection and identification by combination of real-time PCR of transfer-mRNA (ssrA) gene and traditional PCR and sequencing of citrate synthase (gltA) gene. It was shown that 49.52% of the rodents (52/105) were positive for Bartonella spp.. The infection rate in different gender (χ2 = 0.079, P = 0.778) and tissues (χ2 = 0.233, P = 0.890) of small rodents did not have statistical difference, but that in different small rodents (Fisher’s exact test, P < 0.001) and habitats (χ2 = 5.483, P = 0.019) had statistical difference. And, the sequencing data suggests that Bartonella sequences (n = 31) were identified into three species, including 14 of B. grahamii, 3 of B. queenslandensis and 14 of unknown Bartonella species. Phylogenetic analysis showed that B. grahamii sequences were clustered with the isolates from South Korea and China, and B. queenslandensis sequences were mainly closely related to the isolates from China and Thailand. The genetic diversity analysis showed that B. grahamii and B. queenslandensis sequences exhibited noticeable intraspecies diversity. Taken together our data demonstrates the high prevalence and genetic diversity of Bartonella infections in small rodents in the Zhongtiao Mountain, especially a potential novel Bartonella specie was detected, which could benefit the prevention and control of rodent-Bartonella species in this area.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884503/?tool=EBI |
| spellingShingle | Juan Yu Xiong-Ying Zhang Yun-Xia Chen Hong-Bing Cheng Dong-Mei Li Hua-Xiang Rao Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China PLoS ONE |
| title | Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China |
| title_full | Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China |
| title_fullStr | Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China |
| title_full_unstemmed | Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China |
| title_short | Molecular detection and genetic characterization of small rodents associated Bartonella species in Zhongtiao Mountain, China |
| title_sort | molecular detection and genetic characterization of small rodents associated bartonella species in zhongtiao mountain china |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884503/?tool=EBI |
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