Distribution of polyethylenimine in zebrafish embryos
Background. Polyethylenimine (PEI) plays important roles in the pharmaceutical design of non-viral gene delivery systems. Due to a set of unique physicochemical properties this cationic polymer has a great potential in modern gene therapies. Objective. The aim of the present study was to determin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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National Institute of Public Health - National Institute of Hygiene
2018-11-01
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| Series: | Roczniki Panstwowego Zakladu Higieny |
| Subjects: | |
| Online Access: | http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1252 |
| _version_ | 1818640552996896768 |
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| author | Róża Chmielewska Maciej Gawlak Magdalena Bamburowicz-Klimkowska Magdalena Popławska Ireneusz P. Grudziński |
| author_facet | Róża Chmielewska Maciej Gawlak Magdalena Bamburowicz-Klimkowska Magdalena Popławska Ireneusz P. Grudziński |
| author_sort | Róża Chmielewska |
| collection | DOAJ |
| description | Background. Polyethylenimine (PEI) plays important roles in the pharmaceutical design of non-viral gene delivery
systems. Due to a set of unique physicochemical properties this cationic polymer has a great potential in modern gene
therapies.
Objective. The aim of the present study was to determine the distribution of branched PEI (0.8 kDa) in zebrafish embryos
(Danio rerio).
Material and methods. Zebrafish embryos at 3 hours post-fertilization (hpf) were incubated with PEI (10 μg/ml) for 24
and 48 hours and studied using the confocal laser microscopy.
Results. The obtained results show that PEI effectively distributed into the layers of the chorion and yolk sac in developing
embryos due to first 24 hours of exposure. In contrast, PEI was found in the yolk, head, trunk and tail of the embryos due
to prolonged treatments (48 hours).
Conclusion. The study evidences a high distribution of the branched PEI (0.8 kDa) polymer in the zebrafish embryo tissues. |
| first_indexed | 2024-12-16T23:13:06Z |
| format | Article |
| id | doaj.art-fe907492f081498e8d2216d0d5c928c3 |
| institution | Directory Open Access Journal |
| issn | 0035-7715 2451-2311 |
| language | English |
| last_indexed | 2024-12-16T23:13:06Z |
| publishDate | 2018-11-01 |
| publisher | National Institute of Public Health - National Institute of Hygiene |
| record_format | Article |
| series | Roczniki Panstwowego Zakladu Higieny |
| spelling | doaj.art-fe907492f081498e8d2216d0d5c928c32022-12-21T22:12:22ZengNational Institute of Public Health - National Institute of HygieneRoczniki Panstwowego Zakladu Higieny0035-77152451-23112018-11-01693315318Distribution of polyethylenimine in zebrafish embryosRóża Chmielewska0Maciej Gawlak1Magdalena Bamburowicz-Klimkowska2Magdalena Popławska3Ireneusz P. Grudziński4Department of Applied Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Human Physiology and Pathophysiology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Applied Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Organic Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland Department of Applied Toxicology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandBackground. Polyethylenimine (PEI) plays important roles in the pharmaceutical design of non-viral gene delivery systems. Due to a set of unique physicochemical properties this cationic polymer has a great potential in modern gene therapies. Objective. The aim of the present study was to determine the distribution of branched PEI (0.8 kDa) in zebrafish embryos (Danio rerio). Material and methods. Zebrafish embryos at 3 hours post-fertilization (hpf) were incubated with PEI (10 μg/ml) for 24 and 48 hours and studied using the confocal laser microscopy. Results. The obtained results show that PEI effectively distributed into the layers of the chorion and yolk sac in developing embryos due to first 24 hours of exposure. In contrast, PEI was found in the yolk, head, trunk and tail of the embryos due to prolonged treatments (48 hours). Conclusion. The study evidences a high distribution of the branched PEI (0.8 kDa) polymer in the zebrafish embryo tissues.http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1252polyethyleneimine (PEI)distributionzebrafish embryos |
| spellingShingle | Róża Chmielewska Maciej Gawlak Magdalena Bamburowicz-Klimkowska Magdalena Popławska Ireneusz P. Grudziński Distribution of polyethylenimine in zebrafish embryos Roczniki Panstwowego Zakladu Higieny polyethyleneimine (PEI) distribution zebrafish embryos |
| title | Distribution of polyethylenimine in zebrafish embryos |
| title_full | Distribution of polyethylenimine in zebrafish embryos |
| title_fullStr | Distribution of polyethylenimine in zebrafish embryos |
| title_full_unstemmed | Distribution of polyethylenimine in zebrafish embryos |
| title_short | Distribution of polyethylenimine in zebrafish embryos |
| title_sort | distribution of polyethylenimine in zebrafish embryos |
| topic | polyethyleneimine (PEI) distribution zebrafish embryos |
| url | http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1252 |
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