Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study)
We investigated the effect of long-term whey supplementation on biomarkers of B12 status in healthy older adults subjected to different schemes of supplements and exercise. The total study population examined at baseline consisted of 167 healthy older adults (age ≥ 65 year) who were randomized to 1-...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/2072-6643/12/7/2015 |
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| author | Eva Greibe Søren Reitelseder Rasmus L. Bechshøft Jacob Bülow Grith W. Højfeldt Simon R. Schacht Mads L. Knudsen Inge Tetens Marie S. Ostenfeld Ulla R. Mikkelsen Christian W. Heegaard Ebba Nexo Lars Holm |
| author_facet | Eva Greibe Søren Reitelseder Rasmus L. Bechshøft Jacob Bülow Grith W. Højfeldt Simon R. Schacht Mads L. Knudsen Inge Tetens Marie S. Ostenfeld Ulla R. Mikkelsen Christian W. Heegaard Ebba Nexo Lars Holm |
| author_sort | Eva Greibe |
| collection | DOAJ |
| description | We investigated the effect of long-term whey supplementation on biomarkers of B12 status in healthy older adults subjected to different schemes of supplements and exercise. The total study population examined at baseline consisted of 167 healthy older adults (age ≥ 65 year) who were randomized to 1-y intervention with two daily supplements of (1) whey protein (3.1 µg B12/day) (WHEY-ALL), (2) collagen (1.3 µg B12/day) (COLL), or (3) maltodextrin (0.3 µg B12/day) (CARB). WHEY-ALL was comprised of three groups, who performed heavy resistance training (HRTW), light resistance training (LITW), or no training (WHEY). Dietary intake was assessed through 3-d dietary records. For the longitudinal part of the study, we included only the participants (<i>n</i> = 110), who met the criteria of ≥ 50% compliance to the nutritional intervention and ≥ 66% and ≥ 75% compliance to the heavy and light training, respectively. Fasting blood samples collected at baseline and 12 months and non-fasting samples collected at 6 and 18 months were examined for methylmalonic acid, B12 and holotranscobalamin. At baseline, the study population (<i>n</i> = 167) had an overall adequate dietary B12 intake of median (range) 5.3 (0.7–65) µg/day and median B12 biomarker values within reference intervals. The whey intervention (WHEY-ALL) caused an increase in B12 (<i>P</i> < 0.0001) and holotranscobalamin (<i>P</i> < 0.0001). In addition, methylmalonic acid decreased in the LITW group (<i>P</i> = 0.04). No change in B12 biomarkers was observed during the intervention with collagen or carbohydrate, and the training schedules induced no changes. In conclusion, longer-term daily whey intake increased plasma B12 and holotranscobalamin in older individuals. No effect of intervention with collagen or carbohydrate or different training regimes was observed. Interestingly, the biomarkers of B12 status appeared to be affected by fasting vs. non-fasting conditions during sample collection. |
| first_indexed | 2024-03-10T18:39:06Z |
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| issn | 2072-6643 |
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| last_indexed | 2024-03-10T18:39:06Z |
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| spelling | doaj.art-fe907fb994a047b8835984cbebe958f32023-11-20T06:04:35ZengMDPI AGNutrients2072-66432020-07-01127201510.3390/nu12072015Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study)Eva Greibe0Søren Reitelseder1Rasmus L. Bechshøft2Jacob Bülow3Grith W. Højfeldt4Simon R. Schacht5Mads L. Knudsen6Inge Tetens7Marie S. Ostenfeld8Ulla R. Mikkelsen9Christian W. Heegaard10Ebba Nexo11Lars Holm12Department of Clinical Medicine/Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, DK-8200 Aarhus N, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Nielsine Nielsens Vej 11, DK-2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Nielsine Nielsens Vej 11, DK-2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Nielsine Nielsens Vej 11, DK-2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Nielsine Nielsens Vej 11, DK-2400 Copenhagen NV, DenmarkDepartment of Nutrition, Exercise, and Sports, University of Copenhagen, Noerre Alle 51, DK-2200 Copenhagen N, DenmarkDepartment of Nutrition, Exercise, and Sports, University of Copenhagen, Noerre Alle 51, DK-2200 Copenhagen N, DenmarkDepartment of Nutrition, Exercise, and Sports, University of Copenhagen, Noerre Alle 51, DK-2200 Copenhagen N, DenmarkArla Foods Ingredients Group P/S, Soenderhoej 10-12, DK-8260 Viby J, DenmarkArla Foods Ingredients Group P/S, Soenderhoej 10-12, DK-8260 Viby J, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus, DenmarkDepartment of Clinical Medicine/Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, DK-8200 Aarhus N, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Nielsine Nielsens Vej 11, DK-2400 Copenhagen NV, DenmarkWe investigated the effect of long-term whey supplementation on biomarkers of B12 status in healthy older adults subjected to different schemes of supplements and exercise. The total study population examined at baseline consisted of 167 healthy older adults (age ≥ 65 year) who were randomized to 1-y intervention with two daily supplements of (1) whey protein (3.1 µg B12/day) (WHEY-ALL), (2) collagen (1.3 µg B12/day) (COLL), or (3) maltodextrin (0.3 µg B12/day) (CARB). WHEY-ALL was comprised of three groups, who performed heavy resistance training (HRTW), light resistance training (LITW), or no training (WHEY). Dietary intake was assessed through 3-d dietary records. For the longitudinal part of the study, we included only the participants (<i>n</i> = 110), who met the criteria of ≥ 50% compliance to the nutritional intervention and ≥ 66% and ≥ 75% compliance to the heavy and light training, respectively. Fasting blood samples collected at baseline and 12 months and non-fasting samples collected at 6 and 18 months were examined for methylmalonic acid, B12 and holotranscobalamin. At baseline, the study population (<i>n</i> = 167) had an overall adequate dietary B12 intake of median (range) 5.3 (0.7–65) µg/day and median B12 biomarker values within reference intervals. The whey intervention (WHEY-ALL) caused an increase in B12 (<i>P</i> < 0.0001) and holotranscobalamin (<i>P</i> < 0.0001). In addition, methylmalonic acid decreased in the LITW group (<i>P</i> = 0.04). No change in B12 biomarkers was observed during the intervention with collagen or carbohydrate, and the training schedules induced no changes. In conclusion, longer-term daily whey intake increased plasma B12 and holotranscobalamin in older individuals. No effect of intervention with collagen or carbohydrate or different training regimes was observed. Interestingly, the biomarkers of B12 status appeared to be affected by fasting vs. non-fasting conditions during sample collection.https://www.mdpi.com/2072-6643/12/7/2015vitamin B12cobalamininterventionwheywhey protein hydrolysatecarbohydrate |
| spellingShingle | Eva Greibe Søren Reitelseder Rasmus L. Bechshøft Jacob Bülow Grith W. Højfeldt Simon R. Schacht Mads L. Knudsen Inge Tetens Marie S. Ostenfeld Ulla R. Mikkelsen Christian W. Heegaard Ebba Nexo Lars Holm Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study) Nutrients vitamin B12 cobalamin intervention whey whey protein hydrolysate carbohydrate |
| title | Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study) |
| title_full | Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study) |
| title_fullStr | Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study) |
| title_full_unstemmed | Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study) |
| title_short | Effects of Prolonged Whey Protein Supplementation and Resistance Training on Biomarkers of Vitamin B12 Status: A 1-Year Randomized Intervention in Healthy Older Adults (the CALM Study) |
| title_sort | effects of prolonged whey protein supplementation and resistance training on biomarkers of vitamin b12 status a 1 year randomized intervention in healthy older adults the calm study |
| topic | vitamin B12 cobalamin intervention whey whey protein hydrolysate carbohydrate |
| url | https://www.mdpi.com/2072-6643/12/7/2015 |
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