CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice

CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice

Evidence suggests that estradiol-sensing preoptic area GABA neurons are involved in the preovulatory surge mechanism necessary for ovulation. In vivo CRISPR-Cas9 editing was used to achieve a 60–70% knockdown in estrogen receptor alpha (ESR1) expression by GABA neurons located within the regions of...

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Bibliographic Details
Main Authors: Jenny Clarkson, Siew Hoong Yip, Robert Porteous, Alexia Kauff, Alison K Heather, Allan E Herbison
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2023-12-01
Series:eLife
Subjects:
kisspeptin
GABA
fertility
preoptic area
estrogen receptor
Online Access:https://elifesciences.org/articles/90959

Internet

https://elifesciences.org/articles/90959

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