Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma
The most important cause of treatment failure of nasopharyngeal carcinoma (NPC) patients is metastasis, including regional lymph nodes or distant metastasis, resulting in a poor prognosis and challenges for treatment. In the present study, we investigated the in vitro anti- tumoral properties of mor...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/20/4851 |
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| author | Cheng-Chen Huang Po-Hui Wang Yen-Ting Lu Jia-Sin Yang Shun-Fa Yang Yu-Ting Ho Chiao-Wen Lin Chung-Han Hsin |
| author_facet | Cheng-Chen Huang Po-Hui Wang Yen-Ting Lu Jia-Sin Yang Shun-Fa Yang Yu-Ting Ho Chiao-Wen Lin Chung-Han Hsin |
| author_sort | Cheng-Chen Huang |
| collection | DOAJ |
| description | The most important cause of treatment failure of nasopharyngeal carcinoma (NPC) patients is metastasis, including regional lymph nodes or distant metastasis, resulting in a poor prognosis and challenges for treatment. In the present study, we investigated the in vitro anti- tumoral properties of morusin on human nasopharyngeal carcinoma HONE-1, NPC-39, and NPC-BM cells. Our study revealed that morusin suppressed the migration and invasion abilities of the three NPC cells. Gelatin zymography assay and Western blotting demonstrated that the enzyme activity and the level of matrix metalloproteinases-2 (MMP-2) protein were downregulated by the treatment of morusin. Mitogen-activated protein kinase proteins were examined to identify the signaling pathway, which showed that phosphorylation of ERK1/2 was inhibited after the treatment of morusin. In summary, our data showed that morusin inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 by downregulating the ERK1/2 signaling pathway, suggesting that morusin may be a potential candidate for chemoprevention or adjuvant therapy of NPC. |
| first_indexed | 2024-03-10T15:28:07Z |
| format | Article |
| id | doaj.art-fe925b247bfa4c07b6c9b57ae2a0920f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:28:07Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fe925b247bfa4c07b6c9b57ae2a0920f2023-11-20T17:57:14ZengMDPI AGMolecules1420-30492020-10-012520485110.3390/molecules25204851Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal CarcinomaCheng-Chen Huang0Po-Hui Wang1Yen-Ting Lu2Jia-Sin Yang3Shun-Fa Yang4Yu-Ting Ho5Chiao-Wen Lin6Chung-Han Hsin7Institute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanThe most important cause of treatment failure of nasopharyngeal carcinoma (NPC) patients is metastasis, including regional lymph nodes or distant metastasis, resulting in a poor prognosis and challenges for treatment. In the present study, we investigated the in vitro anti- tumoral properties of morusin on human nasopharyngeal carcinoma HONE-1, NPC-39, and NPC-BM cells. Our study revealed that morusin suppressed the migration and invasion abilities of the three NPC cells. Gelatin zymography assay and Western blotting demonstrated that the enzyme activity and the level of matrix metalloproteinases-2 (MMP-2) protein were downregulated by the treatment of morusin. Mitogen-activated protein kinase proteins were examined to identify the signaling pathway, which showed that phosphorylation of ERK1/2 was inhibited after the treatment of morusin. In summary, our data showed that morusin inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 by downregulating the ERK1/2 signaling pathway, suggesting that morusin may be a potential candidate for chemoprevention or adjuvant therapy of NPC.https://www.mdpi.com/1420-3049/25/20/4851morusinnasopharyngeal carcinomametastasisMMP-2 |
| spellingShingle | Cheng-Chen Huang Po-Hui Wang Yen-Ting Lu Jia-Sin Yang Shun-Fa Yang Yu-Ting Ho Chiao-Wen Lin Chung-Han Hsin Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma Molecules morusin nasopharyngeal carcinoma metastasis MMP-2 |
| title | Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma |
| title_full | Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma |
| title_fullStr | Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma |
| title_full_unstemmed | Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma |
| title_short | Morusin Suppresses Cancer Cell Invasion and MMP-2 Expression through ERK Signaling in Human Nasopharyngeal Carcinoma |
| title_sort | morusin suppresses cancer cell invasion and mmp 2 expression through erk signaling in human nasopharyngeal carcinoma |
| topic | morusin nasopharyngeal carcinoma metastasis MMP-2 |
| url | https://www.mdpi.com/1420-3049/25/20/4851 |
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