Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching
Dental bleaching has become one of the most frequently requested esthetic treatments in dental offices. Despite the high clinical success observed with this procedure, some adverse effects have been reported, including a potential for developing premalignant lesions, root resorption and tooth sensit...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Pesquisa Odontológica
2015-01-01
|
| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100217&lng=en&tlng=en |
| _version_ | 1819025353328295936 |
|---|---|
| author | Aline Ferreira de ALMEIDA Eliana do Nascimento TORRE Maicon dos Santos SELAYARAN Fábio Renato Manzolli LEITE Flávio Fernando DEMARCO Alessandro Dourado LOGUERCIO Adriana ETGES |
| author_facet | Aline Ferreira de ALMEIDA Eliana do Nascimento TORRE Maicon dos Santos SELAYARAN Fábio Renato Manzolli LEITE Flávio Fernando DEMARCO Alessandro Dourado LOGUERCIO Adriana ETGES |
| author_sort | Aline Ferreira de ALMEIDA |
| collection | DOAJ |
| description | Dental bleaching has become one of the most frequently requested esthetic treatments in dental offices. Despite the high clinical success observed with this procedure, some adverse effects have been reported, including a potential for developing premalignant lesions, root resorption and tooth sensitivity, especially when misused. The aim of this study was to evaluate the genotoxic response using a micronucleus (MN) assay, after the application of two concentrations of carbamide peroxide. Thirty-seven patients were divided into two groups and randomly received either a 10% carbamide peroxide (CP) (19) or a 16% carbamide peroxide (18) concentration for 21 days in individual dental trays. Gingival margin cells were collected immediately before the first use (baseline), and then 15 and 45 days after baseline. The cells were placed on a histological slide, stained by the Feulgen technique, and evaluated by an experienced blinded examiner. One thousand cells per slide were counted, and the MN rate was determined. The two groups were analyzed by the Wilcoxon rank-sum test and the Kruskal-Wallis equality-of-populations rank test. A slight increase in MN was observed for both groups, in comparison with the baseline, at 15 days. However, no difference was observed between the two groups (10% and 16%), at either 15 or 45 days (p = 0.90). When bleaching is not prolonged or not performed very frequently, bleaching agents containing carbamide peroxide alone will not cause mutagenic stress on gingival epithelial cells. |
| first_indexed | 2024-12-21T05:09:20Z |
| format | Article |
| id | doaj.art-fe974d209d3948548d17595c9015358d |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-21T05:09:20Z |
| publishDate | 2015-01-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-fe974d209d3948548d17595c9015358d2022-12-21T19:15:07ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072015-01-012911710.1590/1807-3107BOR-2015.vol29.0021S1806-83242015000100217Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleachingAline Ferreira de ALMEIDAEliana do Nascimento TORREMaicon dos Santos SELAYARANFábio Renato Manzolli LEITEFlávio Fernando DEMARCOAlessandro Dourado LOGUERCIOAdriana ETGESDental bleaching has become one of the most frequently requested esthetic treatments in dental offices. Despite the high clinical success observed with this procedure, some adverse effects have been reported, including a potential for developing premalignant lesions, root resorption and tooth sensitivity, especially when misused. The aim of this study was to evaluate the genotoxic response using a micronucleus (MN) assay, after the application of two concentrations of carbamide peroxide. Thirty-seven patients were divided into two groups and randomly received either a 10% carbamide peroxide (CP) (19) or a 16% carbamide peroxide (18) concentration for 21 days in individual dental trays. Gingival margin cells were collected immediately before the first use (baseline), and then 15 and 45 days after baseline. The cells were placed on a histological slide, stained by the Feulgen technique, and evaluated by an experienced blinded examiner. One thousand cells per slide were counted, and the MN rate was determined. The two groups were analyzed by the Wilcoxon rank-sum test and the Kruskal-Wallis equality-of-populations rank test. A slight increase in MN was observed for both groups, in comparison with the baseline, at 15 days. However, no difference was observed between the two groups (10% and 16%), at either 15 or 45 days (p = 0.90). When bleaching is not prolonged or not performed very frequently, bleaching agents containing carbamide peroxide alone will not cause mutagenic stress on gingival epithelial cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100217&lng=en&tlng=enTooth BleachingPeroxidesCytologyMicronucleus Tests |
| spellingShingle | Aline Ferreira de ALMEIDA Eliana do Nascimento TORRE Maicon dos Santos SELAYARAN Fábio Renato Manzolli LEITE Flávio Fernando DEMARCO Alessandro Dourado LOGUERCIO Adriana ETGES Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching Brazilian Oral Research Tooth Bleaching Peroxides Cytology Micronucleus Tests |
| title | Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching |
| title_full | Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching |
| title_fullStr | Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching |
| title_full_unstemmed | Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching |
| title_short | Genotoxic potential of 10% and 16% Carbamide Peroxide in dental bleaching |
| title_sort | genotoxic potential of 10 and 16 carbamide peroxide in dental bleaching |
| topic | Tooth Bleaching Peroxides Cytology Micronucleus Tests |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242015000100217&lng=en&tlng=en |
| work_keys_str_mv | AT alineferreiradealmeida genotoxicpotentialof10and16carbamideperoxideindentalbleaching AT elianadonascimentotorre genotoxicpotentialof10and16carbamideperoxideindentalbleaching AT maicondossantosselayaran genotoxicpotentialof10and16carbamideperoxideindentalbleaching AT fabiorenatomanzollileite genotoxicpotentialof10and16carbamideperoxideindentalbleaching AT flaviofernandodemarco genotoxicpotentialof10and16carbamideperoxideindentalbleaching AT alessandrodouradologuercio genotoxicpotentialof10and16carbamideperoxideindentalbleaching AT adrianaetges genotoxicpotentialof10and16carbamideperoxideindentalbleaching |