Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS
Poplar, a woody tree species, is widely used for industrial production and as a protective forest belt. Different clones of poplar exhibit clear variation in terms of morphological and physiological features, however, the impact of the genetic variation on the composition and abundance of wood metab...
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MDPI AG
2023-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/20/7024 |
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| author | Liping Li Yun Liu Xiaorui Yu Xiaoqin Yang Sida Xie Guolei Zhu Ping Zhao |
| author_facet | Liping Li Yun Liu Xiaorui Yu Xiaoqin Yang Sida Xie Guolei Zhu Ping Zhao |
| author_sort | Liping Li |
| collection | DOAJ |
| description | Poplar, a woody tree species, is widely used for industrial production and as a protective forest belt. Different clones of poplar exhibit clear variation in terms of morphological and physiological features, however, the impact of the genetic variation on the composition and abundance of wood metabolite have not been fully determined. In this study, ultra-high pressure liquid chromatography-triple time of flight-mass spectrometer (UPLC-Triple-TOF-MS) was used to explore the metabolite changes in poplar wood from three clones, including <i>Populus deltoides</i> CL. ‘55/65’, <i>P. deltoides</i> CL. ‘Danhong’, and <i>P. nigra</i> CL. ‘N179’. A total of 699 metabolites were identified. Clustering analysis and principal component analysis display that the metabolic differences of wood have allowed distinguishing different species of poplar. Meanwhile, eight significantly different metabolites were screened between <i>P. deltoides</i> and <i>P. nigra</i>, which may be considered as valuable markers for chemotaxonomy. In addition, the highly discriminant 352 metabolites were obtained among the three clones, and those may be closely related to the distinction in unique properties (e.g., growth, rigidity and tolerance) of the poplar wood cultivars. This study provides a foundation for further studies on wood metabolomics in poplar, and offers chemotaxonomic markers that will stimulate the early screening of potentially superior trees. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:01:12Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-fe9a7d02108b4507ba7d3308f4310a572023-11-19T17:31:38ZengMDPI AGMolecules1420-30492023-10-012820702410.3390/molecules28207024Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MSLiping Li0Yun Liu1Xiaorui Yu2Xiaoqin Yang3Sida Xie4Guolei Zhu5Ping Zhao6Key Laboratory of State Forestry and Grassland Administration on Highly Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of State Forestry and Grassland Administration on Highly Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of State Forestry and Grassland Administration on Highly Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of State Forestry and Grassland Administration on Highly Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of State Forestry and Grassland Administration on Highly Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, ChinaKey Laboratory of State Forestry and Grassland Administration on Highly Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming 650224, ChinaPoplar, a woody tree species, is widely used for industrial production and as a protective forest belt. Different clones of poplar exhibit clear variation in terms of morphological and physiological features, however, the impact of the genetic variation on the composition and abundance of wood metabolite have not been fully determined. In this study, ultra-high pressure liquid chromatography-triple time of flight-mass spectrometer (UPLC-Triple-TOF-MS) was used to explore the metabolite changes in poplar wood from three clones, including <i>Populus deltoides</i> CL. ‘55/65’, <i>P. deltoides</i> CL. ‘Danhong’, and <i>P. nigra</i> CL. ‘N179’. A total of 699 metabolites were identified. Clustering analysis and principal component analysis display that the metabolic differences of wood have allowed distinguishing different species of poplar. Meanwhile, eight significantly different metabolites were screened between <i>P. deltoides</i> and <i>P. nigra</i>, which may be considered as valuable markers for chemotaxonomy. In addition, the highly discriminant 352 metabolites were obtained among the three clones, and those may be closely related to the distinction in unique properties (e.g., growth, rigidity and tolerance) of the poplar wood cultivars. This study provides a foundation for further studies on wood metabolomics in poplar, and offers chemotaxonomic markers that will stimulate the early screening of potentially superior trees.https://www.mdpi.com/1420-3049/28/20/7024wood<i>Populus</i>genetic backgroundcomparative metabolomics |
| spellingShingle | Liping Li Yun Liu Xiaorui Yu Xiaoqin Yang Sida Xie Guolei Zhu Ping Zhao Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS Molecules wood <i>Populus</i> genetic background comparative metabolomics |
| title | Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS |
| title_full | Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS |
| title_fullStr | Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS |
| title_full_unstemmed | Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS |
| title_short | Comparative Analysis of the Wood Metabolites of Three Poplar Clones Using UPLC-Triple-TOF-MS |
| title_sort | comparative analysis of the wood metabolites of three poplar clones using uplc triple tof ms |
| topic | wood <i>Populus</i> genetic background comparative metabolomics |
| url | https://www.mdpi.com/1420-3049/28/20/7024 |
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