Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance
A chronic low-grade inflammation, originating in the adipose tissue, is considered a driver of obesity-associated insulin resistance. Macrophage composition in white adipose tissue is believed to contribute to the pathogenesis of metabolic diseases, but a detailed characterization of pro- and anti-i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.856530/full |
| _version_ | 1818009494832021504 |
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| author | Kristina Strand Kristina Strand Natalie Stiglund Martha Eimstad Haugstøyl Martha Eimstad Haugstøyl Zahra Kamyab Zahra Kamyab Victoria Langhelle Victoria Langhelle Laurence Lawrence-Archer Laurence Lawrence-Archer Christian Busch Martin Cornillet Iren Drange Hjellestad Iren Drange Hjellestad Hans Jørgen Nielsen Pål Rasmus Njølstad Pål Rasmus Njølstad Gunnar Mellgren Gunnar Mellgren Niklas K. Björkström Johan Fernø Johan Fernø |
| author_facet | Kristina Strand Kristina Strand Natalie Stiglund Martha Eimstad Haugstøyl Martha Eimstad Haugstøyl Zahra Kamyab Zahra Kamyab Victoria Langhelle Victoria Langhelle Laurence Lawrence-Archer Laurence Lawrence-Archer Christian Busch Martin Cornillet Iren Drange Hjellestad Iren Drange Hjellestad Hans Jørgen Nielsen Pål Rasmus Njølstad Pål Rasmus Njølstad Gunnar Mellgren Gunnar Mellgren Niklas K. Björkström Johan Fernø Johan Fernø |
| author_sort | Kristina Strand |
| collection | DOAJ |
| description | A chronic low-grade inflammation, originating in the adipose tissue, is considered a driver of obesity-associated insulin resistance. Macrophage composition in white adipose tissue is believed to contribute to the pathogenesis of metabolic diseases, but a detailed characterization of pro- and anti-inflammatory adipose tissue macrophages (ATMs) in human obesity and how they are distributed in visceral- and subcutaneous adipose depots is lacking. In this study, we performed a surface proteome screening of pro- and anti-inflammatory ATMs in both subcutaneous- (SAT) and visceral adipose tissue (VAT) and evaluated their relationship with systemic insulin resistance. From the proteomics screen we found novel surface proteins specific to M1-like- and M2-like macrophages, and we identified depot-specific immunophenotypes in SAT and VAT. Furthermore, we found that insulin resistance, assessed by HOMA-IR, was positively associated with a relative increase in pro-inflammatory M1-like macrophages in both SAT and VAT. |
| first_indexed | 2024-04-14T05:43:34Z |
| format | Article |
| id | doaj.art-feaaad9bf8f740d9a6830322a7ee38f0 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-14T05:43:34Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-feaaad9bf8f740d9a6830322a7ee38f02022-12-22T02:09:22ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-04-011310.3389/fendo.2022.856530856530Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin ResistanceKristina Strand0Kristina Strand1Natalie Stiglund2Martha Eimstad Haugstøyl3Martha Eimstad Haugstøyl4Zahra Kamyab5Zahra Kamyab6Victoria Langhelle7Victoria Langhelle8Laurence Lawrence-Archer9Laurence Lawrence-Archer10Christian Busch11Martin Cornillet12Iren Drange Hjellestad13Iren Drange Hjellestad14Hans Jørgen Nielsen15Pål Rasmus Njølstad16Pål Rasmus Njølstad17Gunnar Mellgren18Gunnar Mellgren19Niklas K. Björkström20Johan Fernø21Johan Fernø22Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayPlastikkirurg1, Bergen, NorwayCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayDepartment of Medicine, Haukeland University Hospital, Bergen, NorwayDepartment of Surgery, Voss Hospital, Haukeland University Hospital, Bergen, NorwayCenter for Diabetes Research, Department of Clinical Science, University of Bergen, Bergen, NorwayDepartment of Pediatrics and Adolescents, Haukeland University Hospital, Bergen, NorwayHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenHormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, NorwayMohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, NorwayA chronic low-grade inflammation, originating in the adipose tissue, is considered a driver of obesity-associated insulin resistance. Macrophage composition in white adipose tissue is believed to contribute to the pathogenesis of metabolic diseases, but a detailed characterization of pro- and anti-inflammatory adipose tissue macrophages (ATMs) in human obesity and how they are distributed in visceral- and subcutaneous adipose depots is lacking. In this study, we performed a surface proteome screening of pro- and anti-inflammatory ATMs in both subcutaneous- (SAT) and visceral adipose tissue (VAT) and evaluated their relationship with systemic insulin resistance. From the proteomics screen we found novel surface proteins specific to M1-like- and M2-like macrophages, and we identified depot-specific immunophenotypes in SAT and VAT. Furthermore, we found that insulin resistance, assessed by HOMA-IR, was positively associated with a relative increase in pro-inflammatory M1-like macrophages in both SAT and VAT.https://www.frontiersin.org/articles/10.3389/fendo.2022.856530/fullsurface proteomicsadipose tissue macrophageobesityinsulin resistanceflow cytometry |
| spellingShingle | Kristina Strand Kristina Strand Natalie Stiglund Martha Eimstad Haugstøyl Martha Eimstad Haugstøyl Zahra Kamyab Zahra Kamyab Victoria Langhelle Victoria Langhelle Laurence Lawrence-Archer Laurence Lawrence-Archer Christian Busch Martin Cornillet Iren Drange Hjellestad Iren Drange Hjellestad Hans Jørgen Nielsen Pål Rasmus Njølstad Pål Rasmus Njølstad Gunnar Mellgren Gunnar Mellgren Niklas K. Björkström Johan Fernø Johan Fernø Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance Frontiers in Endocrinology surface proteomics adipose tissue macrophage obesity insulin resistance flow cytometry |
| title | Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance |
| title_full | Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance |
| title_fullStr | Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance |
| title_full_unstemmed | Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance |
| title_short | Subtype-Specific Surface Proteins on Adipose Tissue Macrophages and Their Association to Obesity-Induced Insulin Resistance |
| title_sort | subtype specific surface proteins on adipose tissue macrophages and their association to obesity induced insulin resistance |
| topic | surface proteomics adipose tissue macrophage obesity insulin resistance flow cytometry |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.856530/full |
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