The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients
Abstract Background Adenosine signaling is now an accepted explanation for the therapeutic mechanism of Methotrexate (MTX) in rheumatoid arthritis (RA). Adenosine receptors categorized into four subclasses: adenosine A1 receptor (ADORA1), adenosine 2a receptor (ADORA2a), adenosine 2b receptor (ADORA...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-02-01
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| Series: | Egyptian Rheumatology and Rehabilitation |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43166-021-00107-9 |
| _version_ | 1828135851495784448 |
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| author | Nivine Fathi Darwish Samar Abd Alhamed Tabra Nivin Naeem Baiomy Heba A. Mahmoud Reham A. Mariah Shymaa Ahmed Hablas |
| author_facet | Nivine Fathi Darwish Samar Abd Alhamed Tabra Nivin Naeem Baiomy Heba A. Mahmoud Reham A. Mariah Shymaa Ahmed Hablas |
| author_sort | Nivine Fathi Darwish |
| collection | DOAJ |
| description | Abstract Background Adenosine signaling is now an accepted explanation for the therapeutic mechanism of Methotrexate (MTX) in rheumatoid arthritis (RA). Adenosine receptors categorized into four subclasses: adenosine A1 receptor (ADORA1), adenosine 2a receptor (ADORA2a), adenosine 2b receptor (ADORA2B), and adenosine 3 receptor (ADORA3). Our aim is to check the mRNA expression of two adenosine receptors; ADORA2a and ADORA3 in whole blood cell of RA patients and its relation in prediction of MTX clinical response in Egyptian patients. Results There was significant correlation between both ADORA2a and ADORA3 gene expression in RA patients as compared with healthy controls. The expression of ADORA2a and ADORA3 was increased in good and moderate response groups compared to no response group. There was significant correlation between both genes in mRNA expression before and after MTX treatment. Matrix metalloproteinase-3 (MMP3) concentration was significantly decreased after treatment in good and moderate response groups in comparison to non-responder group. Conclusion The inflammatory and clinical responses in RA patients which is demonstrated by DAS28 and suppression of MMP3 were regulated by ADORA2a and ADORA3. Their level of expression can predict MTX response and their agonists may offer a novel and effective therapeutic option for RA patients. |
| first_indexed | 2024-04-11T17:53:53Z |
| format | Article |
| id | doaj.art-feaf10028f254607aab1f4c791bea807 |
| institution | Directory Open Access Journal |
| issn | 1110-161X 2090-3235 |
| language | English |
| last_indexed | 2024-04-11T17:53:53Z |
| publishDate | 2022-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Rheumatology and Rehabilitation |
| spelling | doaj.art-feaf10028f254607aab1f4c791bea8072022-12-22T04:10:58ZengSpringerOpenEgyptian Rheumatology and Rehabilitation1110-161X2090-32352022-02-014911910.1186/s43166-021-00107-9The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patientsNivine Fathi Darwish0Samar Abd Alhamed Tabra1Nivin Naeem Baiomy2Heba A. Mahmoud3Reham A. Mariah4Shymaa Ahmed Hablas5Physical Medicine, Rheumatology and Rehabilitation, Faculty of Medicine, Tanta UniversityPhysical Medicine, Rheumatology and Rehabilitation, Faculty of Medicine, Tanta UniversityDepartment of Clinical Pathology, Faculty of Medicine Tanta UniversityDepartment of Clinical Pathology, Faculty of Medicine Tanta UniversityDepartment of Clinical Pathology, Faculty of Medicine Tanta UniversityPhysical Medicine, Rheumatology and Rehabilitation, Faculty of Medicine, Tanta UniversityAbstract Background Adenosine signaling is now an accepted explanation for the therapeutic mechanism of Methotrexate (MTX) in rheumatoid arthritis (RA). Adenosine receptors categorized into four subclasses: adenosine A1 receptor (ADORA1), adenosine 2a receptor (ADORA2a), adenosine 2b receptor (ADORA2B), and adenosine 3 receptor (ADORA3). Our aim is to check the mRNA expression of two adenosine receptors; ADORA2a and ADORA3 in whole blood cell of RA patients and its relation in prediction of MTX clinical response in Egyptian patients. Results There was significant correlation between both ADORA2a and ADORA3 gene expression in RA patients as compared with healthy controls. The expression of ADORA2a and ADORA3 was increased in good and moderate response groups compared to no response group. There was significant correlation between both genes in mRNA expression before and after MTX treatment. Matrix metalloproteinase-3 (MMP3) concentration was significantly decreased after treatment in good and moderate response groups in comparison to non-responder group. Conclusion The inflammatory and clinical responses in RA patients which is demonstrated by DAS28 and suppression of MMP3 were regulated by ADORA2a and ADORA3. Their level of expression can predict MTX response and their agonists may offer a novel and effective therapeutic option for RA patients.https://doi.org/10.1186/s43166-021-00107-9Adenosine receptorRheumatoid arthritisMTX responseInflammatory mediators |
| spellingShingle | Nivine Fathi Darwish Samar Abd Alhamed Tabra Nivin Naeem Baiomy Heba A. Mahmoud Reham A. Mariah Shymaa Ahmed Hablas The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients Egyptian Rheumatology and Rehabilitation Adenosine receptor Rheumatoid arthritis MTX response Inflammatory mediators |
| title | The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients |
| title_full | The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients |
| title_fullStr | The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients |
| title_full_unstemmed | The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients |
| title_short | The implication of adenosine receptor expression in prediction of methotrexate clinical response in Egyptian rheumatoid arthritis patients |
| title_sort | implication of adenosine receptor expression in prediction of methotrexate clinical response in egyptian rheumatoid arthritis patients |
| topic | Adenosine receptor Rheumatoid arthritis MTX response Inflammatory mediators |
| url | https://doi.org/10.1186/s43166-021-00107-9 |
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