Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines
Myostatin (MSTN) negatively regulates in muscle growth and development. Among alternative splicing isoforms of avian MSTN, MSTN-A has antimyogenic activities and MSTN-B functions as a promyogenic factor. In this study, different lines of Japanese quail were used: a random bred control (RBC) and a he...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120301085 |
| _version_ | 1819059696370188288 |
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| author | Dong-Hwan Kim Young Min Choi Yeunsu Suh Sangsu Shin Joonbum Lee Seongsoo Hwang Kichoon Lee |
| author_facet | Dong-Hwan Kim Young Min Choi Yeunsu Suh Sangsu Shin Joonbum Lee Seongsoo Hwang Kichoon Lee |
| author_sort | Dong-Hwan Kim |
| collection | DOAJ |
| description | Myostatin (MSTN) negatively regulates in muscle growth and development. Among alternative splicing isoforms of avian MSTN, MSTN-A has antimyogenic activities and MSTN-B functions as a promyogenic factor. In this study, different lines of Japanese quail were used: a random bred control (RBC) and a heavy weight (HW) quail line with muscle hypertrophy. The objectives of the current study are to compare temporal expression of the MSTN isoforms in pectoralis major muscle (PM) between 2 quail lines and to relate MSTN expression with temporal changes in muscle growth and total amounts of DNA in PM. Gains of body weight (BW) and PM weight were greater until posthatch day (D) 28 (P < 0.001), and the fold increases in total DNA contents of PM were greater in the HW line compared with the RBC line during D7 to D28 (P < 0.05). PCR analysis showed that MSTN-A expression was greater at 14 D (E14) of embryonic age (P < 0.01), D7 (P = 0.052), and D14 (P < 0.01) in the RBC line compared with the HW line. At D28 and D75, expression of MSTN-A was greater in the HW line compared with the RBC line (P < 0.05). MSTN-B expression was barely detectable from E14 to D14 and measurable from D28 to D75 in the muscle of both lines. Ratios of the MSTN-B/-A form ranging from 0.15 to 0.29 indicate a minor expression of the B form. Taken together, the lesser expression levels of MSTN-A at E14, D7, and D14 are associated with the fast growth of PM, and greater MSTN-A expression at D28 and D75 are associated with a slowdown of PM growth in the HW line. These data indicate a negative association of MSTN expression with PM growth and provide a scientific basis for potential usage of MSTN expression as a selection marker for greater muscle growth in poultry. |
| first_indexed | 2024-12-21T14:15:12Z |
| format | Article |
| id | doaj.art-feb138cbc49046edb9d9e4d100c30f2e |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-21T14:15:12Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-feb138cbc49046edb9d9e4d100c30f2e2022-12-21T19:00:56ZengElsevierPoultry Science0032-57912020-06-0199629262930Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail linesDong-Hwan Kim0Young Min Choi1Yeunsu Suh2Sangsu Shin3Joonbum Lee4Seongsoo Hwang5Kichoon Lee6Department of Animal Sciences, The Ohio State University, Columbus OH 43210, The United StatesDepartment of Animal Sciences, Kyungpook National University, Sangju 37224, South KoreaDepartment of Animal Sciences, The Ohio State University, Columbus OH 43210, The United StatesDepartment of Animal Biotechnology, Kyungpook National University, Sangju 37224, South KoreaDepartment of Animal Sciences, The Ohio State University, Columbus OH 43210, The United States; Interdisciplinary Ph.D. Program in Nutrition, The Ohio State University, Columbus, OH 43210, The United StatesAnimal Biotechnology Division, National Institute of Animal Science, RDA, Wanju-gun, Jeonbuk 55365, Republic of KoreaDepartment of Animal Sciences, The Ohio State University, Columbus OH 43210, The United States; Interdisciplinary Ph.D. Program in Nutrition, The Ohio State University, Columbus, OH 43210, The United States; Corresponding author:Myostatin (MSTN) negatively regulates in muscle growth and development. Among alternative splicing isoforms of avian MSTN, MSTN-A has antimyogenic activities and MSTN-B functions as a promyogenic factor. In this study, different lines of Japanese quail were used: a random bred control (RBC) and a heavy weight (HW) quail line with muscle hypertrophy. The objectives of the current study are to compare temporal expression of the MSTN isoforms in pectoralis major muscle (PM) between 2 quail lines and to relate MSTN expression with temporal changes in muscle growth and total amounts of DNA in PM. Gains of body weight (BW) and PM weight were greater until posthatch day (D) 28 (P < 0.001), and the fold increases in total DNA contents of PM were greater in the HW line compared with the RBC line during D7 to D28 (P < 0.05). PCR analysis showed that MSTN-A expression was greater at 14 D (E14) of embryonic age (P < 0.01), D7 (P = 0.052), and D14 (P < 0.01) in the RBC line compared with the HW line. At D28 and D75, expression of MSTN-A was greater in the HW line compared with the RBC line (P < 0.05). MSTN-B expression was barely detectable from E14 to D14 and measurable from D28 to D75 in the muscle of both lines. Ratios of the MSTN-B/-A form ranging from 0.15 to 0.29 indicate a minor expression of the B form. Taken together, the lesser expression levels of MSTN-A at E14, D7, and D14 are associated with the fast growth of PM, and greater MSTN-A expression at D28 and D75 are associated with a slowdown of PM growth in the HW line. These data indicate a negative association of MSTN expression with PM growth and provide a scientific basis for potential usage of MSTN expression as a selection marker for greater muscle growth in poultry.http://www.sciencedirect.com/science/article/pii/S0032579120301085myostatinhypertrophyHW quailmuscle developmentMSTN isoforms |
| spellingShingle | Dong-Hwan Kim Young Min Choi Yeunsu Suh Sangsu Shin Joonbum Lee Seongsoo Hwang Kichoon Lee Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines Poultry Science myostatin hypertrophy HW quail muscle development MSTN isoforms |
| title | Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines |
| title_full | Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines |
| title_fullStr | Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines |
| title_full_unstemmed | Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines |
| title_short | Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines |
| title_sort | research note association of temporal expression of myostatin with hypertrophic muscle growth in different japanese quail lines |
| topic | myostatin hypertrophy HW quail muscle development MSTN isoforms |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120301085 |
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