Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures
Conjugation means transfer of genetic material via bacterial membranes. Fimbrial characters that are responsible for this transfer are coded by conjugative plasmids that multiply in the cytoplasm. This transfer efficiency may be different in solid, liquid phases or on membranes. Conjugation in some...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2000-12-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
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| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-4-246-250.pdf |
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| author | Sibel GERGİN GÜNDEŞ Aynur KARADENİZLİ Fethiye KOLAYLI Haluk VAHABOĞLU Ayşe WILLKE |
| author_facet | Sibel GERGİN GÜNDEŞ Aynur KARADENİZLİ Fethiye KOLAYLI Haluk VAHABOĞLU Ayşe WILLKE |
| author_sort | Sibel GERGİN GÜNDEŞ |
| collection | DOAJ |
| description | Conjugation means transfer of genetic material via bacterial membranes. Fimbrial characters that are responsible for this transfer are coded by conjugative plasmids that multiply in the cytoplasm. This transfer efficiency may be different in solid, liquid phases or on membranes. Conjugation in some of the members of Enterobacteriaceae family is also temperature dependant. This study was carried out to determine the optimal temperature and donor-acceptor ratios for transconjugation in Escherichia coli, Klebsiella and Salmonella species which belong to Enterobacteriaceae family. Fifteen strains, five from each of these species that mentioned above, which were determined to be multiresistant, were introduced to standart recipient E. coli J53-2 in two different concentration [(a) donor:recipient ratio 4:1; and (b) donor:recipient ratio is 1:4] and three different temperatures 32°C, 37°C and 42°C. “Multivariate” analysis was done with “repeated measures one way ANOVA test” whereas “univariate” analysis was done with “Wicoxon Sign Rank” tests. The results of these two tests correlated well and the results were found to be statistically different. In conclusion, transconjugation on solid phase was found to be optimal for group (b) where the recipient:donor ratio was 4:1 and the temperature was 37°C. |
| first_indexed | 2024-04-10T11:17:26Z |
| format | Article |
| id | doaj.art-feb419a2fe1a4770b338c8bc37f87778 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T11:17:26Z |
| publishDate | 2000-12-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-feb419a2fe1a4770b338c8bc37f877782023-02-15T16:18:48ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2000-12-0154246250Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different TemperaturesSibel GERGİN GÜNDEŞ0Aynur KARADENİZLİ1Fethiye KOLAYLI2Haluk VAHABOĞLU3Ayşe WILLKE4Kocaeli Üniversitesi Tıp Fakültesi, Klinik Bakteriyoloji ve İnfeksiyon Hastalıkları Anabilim Dalı,Kocaeli Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İZMİTKocaeli Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, İZMİTKocaeli Üniversitesi Tıp Fakültesi, Klinik Bakteriyoloji ve İnfeksiyon Hastalıkları Anabilim Dalı,Kocaeli Üniversitesi Tıp Fakültesi, Klinik Bakteriyoloji ve İnfeksiyon Hastalıkları Anabilim Dalı,Conjugation means transfer of genetic material via bacterial membranes. Fimbrial characters that are responsible for this transfer are coded by conjugative plasmids that multiply in the cytoplasm. This transfer efficiency may be different in solid, liquid phases or on membranes. Conjugation in some of the members of Enterobacteriaceae family is also temperature dependant. This study was carried out to determine the optimal temperature and donor-acceptor ratios for transconjugation in Escherichia coli, Klebsiella and Salmonella species which belong to Enterobacteriaceae family. Fifteen strains, five from each of these species that mentioned above, which were determined to be multiresistant, were introduced to standart recipient E. coli J53-2 in two different concentration [(a) donor:recipient ratio 4:1; and (b) donor:recipient ratio is 1:4] and three different temperatures 32°C, 37°C and 42°C. “Multivariate” analysis was done with “repeated measures one way ANOVA test” whereas “univariate” analysis was done with “Wicoxon Sign Rank” tests. The results of these two tests correlated well and the results were found to be statistically different. In conclusion, transconjugation on solid phase was found to be optimal for group (b) where the recipient:donor ratio was 4:1 and the temperature was 37°C.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-4-246-250.pdfTransconjugationEscherichia coliKlebsiellaSalmonella |
| spellingShingle | Sibel GERGİN GÜNDEŞ Aynur KARADENİZLİ Fethiye KOLAYLI Haluk VAHABOĞLU Ayşe WILLKE Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Transconjugation Escherichia coli Klebsiella Salmonella |
| title | Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures |
| title_full | Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures |
| title_fullStr | Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures |
| title_full_unstemmed | Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures |
| title_short | Evaluation of Resistance Transfer by Conjugation, in Escherichia coli, Klebsiella and Salmonella Species, on Solid Phase at Different Temperatures |
| title_sort | evaluation of resistance transfer by conjugation in escherichia coli klebsiella and salmonella species on solid phase at different temperatures |
| topic | Transconjugation Escherichia coli Klebsiella Salmonella |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2000-5-4-246-250.pdf |
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