Two-dimensional gel proteome reference map of blood monocytes
<p>Abstract</p> <p>Background:</p> <p>Blood monocytes play a central role in regulating host inflammatory processes through chemotaxis, phagocytosis, and cytokine production. However, the molecular details underlying these diverse functions are not completely understood...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-09-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/4/1/16 |
| _version_ | 1818379740357066752 |
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| author | Eng Charis Bourgeois Tran Diaz Philip T Jin Ming Marsh Clay B Wu Haifeng M |
| author_facet | Eng Charis Bourgeois Tran Diaz Philip T Jin Ming Marsh Clay B Wu Haifeng M |
| author_sort | Eng Charis |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background:</p> <p>Blood monocytes play a central role in regulating host inflammatory processes through chemotaxis, phagocytosis, and cytokine production. However, the molecular details underlying these diverse functions are not completely understood. Understanding the proteomes of blood monocytes will provide new insights into their biological role in health and diseases.</p> <p>Results:</p> <p>In this study, monocytes were isolated from five healthy donors. Whole monocyte lysates from each donor were then analyzed by 2D gel electrophoresis, and proteins were detected using Sypro Ruby fluorescence and then examined for phosphoproteomes using ProQ phospho-protein fluorescence dye. Between 1525 and 1769 protein spots on each 2D gel were matched, analyzed, and quantified. Abundant protein spots were then subjected to analysis by mass spectrometry. This report describes the protein identities of 231 monocyte protein spots, which represent 164 distinct proteins and their respective isoforms or subunits. Some of these proteins had not been previously characterized at the protein level in monocytes. Among the 231 protein spots, 19 proteins revealed distinct modification by protein phosphorylation.</p> <p>Conclusion:</p> <p>The results of this study offer the most detailed monocyte proteomic database to date and provide new perspectives into the study of monocyte biology.</p> |
| first_indexed | 2024-12-14T02:07:35Z |
| format | Article |
| id | doaj.art-febb02fb495f40569143f72be46482c1 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-14T02:07:35Z |
| publishDate | 2006-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-febb02fb495f40569143f72be46482c12022-12-21T23:20:52ZengBMCProteome Science1477-59562006-09-01411610.1186/1477-5956-4-16Two-dimensional gel proteome reference map of blood monocytesEng CharisBourgeois TranDiaz Philip TJin MingMarsh Clay BWu Haifeng M<p>Abstract</p> <p>Background:</p> <p>Blood monocytes play a central role in regulating host inflammatory processes through chemotaxis, phagocytosis, and cytokine production. However, the molecular details underlying these diverse functions are not completely understood. Understanding the proteomes of blood monocytes will provide new insights into their biological role in health and diseases.</p> <p>Results:</p> <p>In this study, monocytes were isolated from five healthy donors. Whole monocyte lysates from each donor were then analyzed by 2D gel electrophoresis, and proteins were detected using Sypro Ruby fluorescence and then examined for phosphoproteomes using ProQ phospho-protein fluorescence dye. Between 1525 and 1769 protein spots on each 2D gel were matched, analyzed, and quantified. Abundant protein spots were then subjected to analysis by mass spectrometry. This report describes the protein identities of 231 monocyte protein spots, which represent 164 distinct proteins and their respective isoforms or subunits. Some of these proteins had not been previously characterized at the protein level in monocytes. Among the 231 protein spots, 19 proteins revealed distinct modification by protein phosphorylation.</p> <p>Conclusion:</p> <p>The results of this study offer the most detailed monocyte proteomic database to date and provide new perspectives into the study of monocyte biology.</p>http://www.proteomesci.com/content/4/1/16 |
| spellingShingle | Eng Charis Bourgeois Tran Diaz Philip T Jin Ming Marsh Clay B Wu Haifeng M Two-dimensional gel proteome reference map of blood monocytes Proteome Science |
| title | Two-dimensional gel proteome reference map of blood monocytes |
| title_full | Two-dimensional gel proteome reference map of blood monocytes |
| title_fullStr | Two-dimensional gel proteome reference map of blood monocytes |
| title_full_unstemmed | Two-dimensional gel proteome reference map of blood monocytes |
| title_short | Two-dimensional gel proteome reference map of blood monocytes |
| title_sort | two dimensional gel proteome reference map of blood monocytes |
| url | http://www.proteomesci.com/content/4/1/16 |
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