Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis

Klebsiella Phage KP34 RNA Polymerase and Its Use in RNA Synthesis

We have characterized the single subunit RNA polymerase from Klebsiella phage KP34. The enzyme is unique among known bacteriophage RNA polymerases in that it recognizes two unrelated promoter sequences, which provided clues for the evolution of phage single-subunit RNA polymerases. As the first repr...

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Bibliographic Details
Main Authors: Xueling Lu, Hui Wu, Heng Xia, Fengtao Huang, Yan Yan, Bingbing Yu, Rui Cheng, Zuzanna Drulis-Kawa, Bin Zhu
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Microbiology
Subjects:
T7 RNA polymerase
phiKMV
sgRNA
in vitro transcription
run-off RNA synthesis
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.02487/full

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