Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling
Spirulina is a popular edible blue-green alga that has piqued the interest of aqua-feed formulations due to its high concentration of micronutrients, macronutrients, and antioxidants. To investigate the impacts of natural Spirulina as a feed additive on Stinging catfish (Heteropneustes fossilis) gro...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002661 |
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| author | Mobasshsirin Rahman Md. Abdullah Al Mamun Sanjay Singh Rathore Shishir Kumar Nandi Zulhisyam Abdul Kari Lee Seong Wei Albaris B. Tahiluddin Md. Moshiur Rahman Nesara Kadadakatte Manjappa Arman Hossain Shamima Nasren M.M. Mahbub Alam Walter G. Bottje Guillermo Téllez-Isaías Muhammad Anamul Kabir |
| author_facet | Mobasshsirin Rahman Md. Abdullah Al Mamun Sanjay Singh Rathore Shishir Kumar Nandi Zulhisyam Abdul Kari Lee Seong Wei Albaris B. Tahiluddin Md. Moshiur Rahman Nesara Kadadakatte Manjappa Arman Hossain Shamima Nasren M.M. Mahbub Alam Walter G. Bottje Guillermo Téllez-Isaías Muhammad Anamul Kabir |
| author_sort | Mobasshsirin Rahman |
| collection | DOAJ |
| description | Spirulina is a popular edible blue-green alga that has piqued the interest of aqua-feed formulations due to its high concentration of micronutrients, macronutrients, and antioxidants. To investigate the impacts of natural Spirulina as a feed additive on Stinging catfish (Heteropneustes fossilis) growth, feed utilization, hematology and serum biochemistry, gut morphology, as well as disease resistance to Aeromonas hydrophila, a 60-day feeding experiment was performed. The fish (N = 240), with an initial weight of 4.23 ± 0.50 g, were placed in 12 aquaria tanks (100 L/ tank) and fed twice daily until they were satisfied. Four different experimental diets: 0% (D0, control diet), 1% (D1), 3% (D2), and 5% (D3) natural Spirulina meal were used to feed the fish. This study found that Stinging catfish fed with a 5% Spirulina diet obtained notably higher (p < 0.05) specific growth rate, final weight, weight gain, and average daily weight gain than other supplemented regimens. Furthermore, as Spirulina inclusion in diets increased, feed utilization parameters such as feed conversion ratio and protein efficiency ratio improved remarkably (p < 0.05). Red blood cell, white blood cell, lymphocytes, neutrophil, monocytes, basophil, hemoglobin, red cell distribution width-standard deviation, and mean corpuscular hemoglobin were all substantially (p < 0.05) greater in 5% Spirulina diet-fed fish. The basal diet-fed fish had lower (p < 0.05) total protein, globulin, and albumin levels and higher glucose, cholesterol, and triglyceride levels than those fed with other test diets. Compared to other fish groups, light microscopic examination of intestinal tissues revealed that fish fed 3% and 5% Spirulina had well-organized enterocytes, intact epithelial barrier, abundant goblet cells, and lacking luminal cell debris, as well as exhibited no signs of inflammation (edema). Spirulina diets significantly (p < 0.05) affected intestinal villi height and width, mucosa width, crypt depth, villi, and lumen area. Furthermore, a 5% Spirulina diet-fed fish demonstrated remarkably (p < 0.05) greater resistance (68.57%) to A. hydrophila during the bacterial challenge trial. Based on the findings, applying natural Spirulina powder at 5% in diets as a feed supplement could benefit H. fossilis nutrient utilization, growth performance, health status, as well as disease resistance. |
| first_indexed | 2024-03-11T22:32:27Z |
| format | Article |
| id | doaj.art-fec35c7617964e7da212547359a27d72 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T22:32:27Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj.art-fec35c7617964e7da212547359a27d722023-09-23T05:11:28ZengElsevierAquaculture Reports2352-51342023-10-0132101727Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerlingMobasshsirin Rahman0Md. Abdullah Al Mamun1Sanjay Singh Rathore2Shishir Kumar Nandi3Zulhisyam Abdul Kari4Lee Seong Wei5Albaris B. Tahiluddin6Md. Moshiur Rahman7Nesara Kadadakatte Manjappa8Arman Hossain9Shamima Nasren10M.M. Mahbub Alam11Walter G. Bottje12Guillermo Téllez-Isaías13Muhammad Anamul Kabir14Laboratory of Fish Diseases Diagnosis and Pharmacology, Department of Fish Health Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, BangladeshLaboratory of Fish Diseases Diagnosis and Pharmacology, Department of Fish Health Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, Bangladesh; Corresponding author.Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, Karnataka, IndiaDepartment of Aquaculture, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Agricultural Sciences, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus, Jeli 17600, Malaysia; Advanced Livestock and Aquaculture Research Group, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jeli, Kelantan, Malaysia; Corresponding author at: Department of Agricultural Sciences, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus, Jeli 17600, Malaysia.Department of Agricultural Sciences, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus, Jeli 17600, Malaysia; Advanced Livestock and Aquaculture Research Group, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jeli, Kelantan, MalaysiaCollege of Fisheries, Mindanao State University-Tawi-Tawi College of Technology and Oceanography, Sanga-Sanga, Bongao, Tawi-Tawi 7500, Philippines; Department of Aquaculture, Institute of Science, Kastamonu University, Kastamonu 37200, TürkiyeLaboratory of Fish Diseases Diagnosis and Pharmacology, Department of Fish Health Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, BangladeshACI Godrej Agrovet Pvt. Ltd., Rajshahi, BangladeshLaboratory of Fish Diseases Diagnosis and Pharmacology, Department of Fish Health Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Fish Biology and Genetics, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, BangladeshLaboratory of Fish Diseases Diagnosis and Pharmacology, Department of Fish Health Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, BangladeshDepartment of Poultry science, University of Arkansas, Fayetteville, AR 72701, USADepartment of Poultry science, University of Arkansas, Fayetteville, AR 72701, USADepartment of Aquaculture, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, Bangladesh; Advanced Livestock and Aquaculture Research Group, Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jeli, Kelantan, Malaysia; Corresponding author at: Department of Aquaculture, Faculty of Fisheries, Sylhet Agricultural University, Sylhet 3100, Bangladesh.Spirulina is a popular edible blue-green alga that has piqued the interest of aqua-feed formulations due to its high concentration of micronutrients, macronutrients, and antioxidants. To investigate the impacts of natural Spirulina as a feed additive on Stinging catfish (Heteropneustes fossilis) growth, feed utilization, hematology and serum biochemistry, gut morphology, as well as disease resistance to Aeromonas hydrophila, a 60-day feeding experiment was performed. The fish (N = 240), with an initial weight of 4.23 ± 0.50 g, were placed in 12 aquaria tanks (100 L/ tank) and fed twice daily until they were satisfied. Four different experimental diets: 0% (D0, control diet), 1% (D1), 3% (D2), and 5% (D3) natural Spirulina meal were used to feed the fish. This study found that Stinging catfish fed with a 5% Spirulina diet obtained notably higher (p < 0.05) specific growth rate, final weight, weight gain, and average daily weight gain than other supplemented regimens. Furthermore, as Spirulina inclusion in diets increased, feed utilization parameters such as feed conversion ratio and protein efficiency ratio improved remarkably (p < 0.05). Red blood cell, white blood cell, lymphocytes, neutrophil, monocytes, basophil, hemoglobin, red cell distribution width-standard deviation, and mean corpuscular hemoglobin were all substantially (p < 0.05) greater in 5% Spirulina diet-fed fish. The basal diet-fed fish had lower (p < 0.05) total protein, globulin, and albumin levels and higher glucose, cholesterol, and triglyceride levels than those fed with other test diets. Compared to other fish groups, light microscopic examination of intestinal tissues revealed that fish fed 3% and 5% Spirulina had well-organized enterocytes, intact epithelial barrier, abundant goblet cells, and lacking luminal cell debris, as well as exhibited no signs of inflammation (edema). Spirulina diets significantly (p < 0.05) affected intestinal villi height and width, mucosa width, crypt depth, villi, and lumen area. Furthermore, a 5% Spirulina diet-fed fish demonstrated remarkably (p < 0.05) greater resistance (68.57%) to A. hydrophila during the bacterial challenge trial. Based on the findings, applying natural Spirulina powder at 5% in diets as a feed supplement could benefit H. fossilis nutrient utilization, growth performance, health status, as well as disease resistance.http://www.sciencedirect.com/science/article/pii/S2352513423002661SpirulinaStinging catfishFeed utilizationHealth statusBacterial challengeSustainable aquaculture |
| spellingShingle | Mobasshsirin Rahman Md. Abdullah Al Mamun Sanjay Singh Rathore Shishir Kumar Nandi Zulhisyam Abdul Kari Lee Seong Wei Albaris B. Tahiluddin Md. Moshiur Rahman Nesara Kadadakatte Manjappa Arman Hossain Shamima Nasren M.M. Mahbub Alam Walter G. Bottje Guillermo Téllez-Isaías Muhammad Anamul Kabir Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling Aquaculture Reports Spirulina Stinging catfish Feed utilization Health status Bacterial challenge Sustainable aquaculture |
| title | Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling |
| title_full | Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling |
| title_fullStr | Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling |
| title_full_unstemmed | Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling |
| title_short | Effects of dietary supplementation of natural Spirulina on growth performance, hemato-biochemical indices, gut health, and disease resistance to Aeromonas hydrophila of Stinging catfish (Heteropneustes fossilis) fingerling |
| title_sort | effects of dietary supplementation of natural spirulina on growth performance hemato biochemical indices gut health and disease resistance to aeromonas hydrophila of stinging catfish heteropneustes fossilis fingerling |
| topic | Spirulina Stinging catfish Feed utilization Health status Bacterial challenge Sustainable aquaculture |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002661 |
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