Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization
The innate immune system plays a pivotal role in defending invasion of microorganisms for scallops. Previous studies on immune-related genes in the Yesso scallop, Patinopecten yessoensis (Jay, 1857) have mainly focused on characterization and expression pattern in response to bacterial challenge, no...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2016-10-01
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| Series: | Comparative Cytogenetics |
| Online Access: | http://compcytogen.pensoft.net/articles.php?id=10047 |
| _version_ | 1811265412454678528 |
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| author | Zujing Yang Xuan Li Huan Liao Liping Hu Zhengrui Zhang Bosong Zhao Xiaoting Huang Zhenmin Bao |
| author_facet | Zujing Yang Xuan Li Huan Liao Liping Hu Zhengrui Zhang Bosong Zhao Xiaoting Huang Zhenmin Bao |
| author_sort | Zujing Yang |
| collection | DOAJ |
| description | The innate immune system plays a pivotal role in defending invasion of microorganisms for scallops. Previous studies on immune-related genes in the Yesso scallop, Patinopecten yessoensis (Jay, 1857) have mainly focused on characterization and expression pattern in response to bacterial challenge, no research has been carried out on the cytogenetic level yet. In the present study, eight fosmid clones containing the sequences of key immune-related genes (PyNFkB, PyTRAF2, PyTRAF4, PyTRAF7, PyMyd88-1, PyMyd88-3, PyMKK-7 and PyTNFR) were isolated and seven of them were successfully mapped on chromosomes of P. yessoensis utilizing fluorescence in situ hybridization. Wherein, PyMyd88-1, PyMyd88-3 and PyMKK-7 located on the same chromosome pair with adjacent positions and the other genes were mapped on four non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of such genes of the Yesso scallop will lay a foundation for studies such as assignment of interested genes to chromosomes, construction cytogenetic maps and so on. |
| first_indexed | 2024-04-12T20:22:10Z |
| format | Article |
| id | doaj.art-fec4de6614e64d239a9f6b4b06cdd339 |
| institution | Directory Open Access Journal |
| issn | 1993-0771 1993-078X |
| language | English |
| last_indexed | 2024-04-12T20:22:10Z |
| publishDate | 2016-10-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Comparative Cytogenetics |
| spelling | doaj.art-fec4de6614e64d239a9f6b4b06cdd3392022-12-22T03:17:58ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2016-10-0110452954110.3897/CompCytogen.v10i4.1004710047Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridizationZujing Yang0Xuan Li1Huan Liao2Liping Hu3Zhengrui Zhang4Bosong Zhao5Xiaoting Huang6Zhenmin Bao7Ocean University of ChinaOcean University of ChinaOcean University of ChinaYantai Fisheries Research InstituteOcean University of ChinaSchool of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityOcean University of ChinaOcean University of ChinaThe innate immune system plays a pivotal role in defending invasion of microorganisms for scallops. Previous studies on immune-related genes in the Yesso scallop, Patinopecten yessoensis (Jay, 1857) have mainly focused on characterization and expression pattern in response to bacterial challenge, no research has been carried out on the cytogenetic level yet. In the present study, eight fosmid clones containing the sequences of key immune-related genes (PyNFkB, PyTRAF2, PyTRAF4, PyTRAF7, PyMyd88-1, PyMyd88-3, PyMKK-7 and PyTNFR) were isolated and seven of them were successfully mapped on chromosomes of P. yessoensis utilizing fluorescence in situ hybridization. Wherein, PyMyd88-1, PyMyd88-3 and PyMKK-7 located on the same chromosome pair with adjacent positions and the other genes were mapped on four non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of such genes of the Yesso scallop will lay a foundation for studies such as assignment of interested genes to chromosomes, construction cytogenetic maps and so on.http://compcytogen.pensoft.net/articles.php?id=10047 |
| spellingShingle | Zujing Yang Xuan Li Huan Liao Liping Hu Zhengrui Zhang Bosong Zhao Xiaoting Huang Zhenmin Bao Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization Comparative Cytogenetics |
| title | Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization |
| title_full | Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization |
| title_fullStr | Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization |
| title_full_unstemmed | Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization |
| title_short | Physical mapping of immune-related genes in Yesso scallop (Patinopecten yessoensis) using fluorescent in situ hybridization |
| title_sort | physical mapping of immune related genes in yesso scallop patinopecten yessoensis using fluorescent in situ hybridization |
| url | http://compcytogen.pensoft.net/articles.php?id=10047 |
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