Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract
Background: Diabetes mellitus (DM) is widely reported to have adverse effect on most systems of the body. This study investigated the effects of DM on male reproductive parameters and possible role of aqueous leave extract from Basella alba in ameliorating such effects. Methods: Male rats (n = 40) a...
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Kerman University of Medical Sciences
2017-11-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_59761_0e9de7e191ca704214299d5835a73c2c.pdf |
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| author | Dennis Arokoyo Ibukun P. Oyeyipo Stefan S. DU Plessis Novel N. Chegou Yapo G. Aboua |
| author_facet | Dennis Arokoyo Ibukun P. Oyeyipo Stefan S. DU Plessis Novel N. Chegou Yapo G. Aboua |
| author_sort | Dennis Arokoyo |
| collection | DOAJ |
| description | Background: Diabetes mellitus (DM) is widely reported to have adverse effect on most systems of the body. This study investigated the effects of DM on male reproductive parameters and possible role of aqueous leave extract from Basella alba in ameliorating such effects. Methods: Male rats (n = 40) aged 8-10 weeks were randomly divided into 4 equal groups: Healthy Control (HC), Diabetic Control (DC), Healthy Treatment (HT) and Diabetic Treatment (DT). Diabetes was induced in animal by using (STZ single intraperitoneal injection of streptozotocin (STZ), (55 mg/kg). Treatment was oral via gavage HC and DC groups received 0.5 ml/100 g normal saline daily via oral gavage, HT and DT groups received 200 mg/kg plant extract daily for 4 weeks. Fasting blood sugar and body weights were recorded weekly. Animals were sacrificed upon completion of treatments and tissues were collected for further analysis. Results: There was significant decrease in weights of the body, testis, and epididymis in DC compared to HC rats (P < 0.05). Relative testicular weight was significantly increased in both DC and DT compared to HC groups (P < 0.0001). Sperm concentration, viability and morphology, were all significantly reduced in DC versus HC rats (p <0.0001), but improved in DT versus DC (P < 0.05). Histopathological examination showed degenerative changes in DC specimens that were alleviated in DT rats. Conclusion: Aqueous extract of Basella alba plays a major role in ameliorating male reproductive complications due to streptozotocin-induced diabetes mellitus. |
| first_indexed | 2024-03-13T02:08:42Z |
| format | Article |
| id | doaj.art-fec5bb2d0fa943d38156677522a58e77 |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:08:42Z |
| publishDate | 2017-11-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-fec5bb2d0fa943d38156677522a58e772023-07-01T05:22:34ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432017-11-0124646747959761Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave ExtractDennis Arokoyo0Ibukun P. Oyeyipo1Stefan S. DU Plessis2Novel N. Chegou3Yapo G. Aboua4Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, Cape Town, South AfricaDivision of Medical Physiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South AfricaProfessor, Division of Medical Physiology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South AfricaDST/NRF Centre of Excellence for Biomedical Tuberculosis Research and SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics,Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, Cape Town, South AfricaBackground: Diabetes mellitus (DM) is widely reported to have adverse effect on most systems of the body. This study investigated the effects of DM on male reproductive parameters and possible role of aqueous leave extract from Basella alba in ameliorating such effects. Methods: Male rats (n = 40) aged 8-10 weeks were randomly divided into 4 equal groups: Healthy Control (HC), Diabetic Control (DC), Healthy Treatment (HT) and Diabetic Treatment (DT). Diabetes was induced in animal by using (STZ single intraperitoneal injection of streptozotocin (STZ), (55 mg/kg). Treatment was oral via gavage HC and DC groups received 0.5 ml/100 g normal saline daily via oral gavage, HT and DT groups received 200 mg/kg plant extract daily for 4 weeks. Fasting blood sugar and body weights were recorded weekly. Animals were sacrificed upon completion of treatments and tissues were collected for further analysis. Results: There was significant decrease in weights of the body, testis, and epididymis in DC compared to HC rats (P < 0.05). Relative testicular weight was significantly increased in both DC and DT compared to HC groups (P < 0.0001). Sperm concentration, viability and morphology, were all significantly reduced in DC versus HC rats (p <0.0001), but improved in DT versus DC (P < 0.05). Histopathological examination showed degenerative changes in DC specimens that were alleviated in DT rats. Conclusion: Aqueous extract of Basella alba plays a major role in ameliorating male reproductive complications due to streptozotocin-induced diabetes mellitus.https://jkmu.kmu.ac.ir/article_59761_0e9de7e191ca704214299d5835a73c2c.pdfmale reproductionstreptozotocindiabetesbasella albaaqueous extract |
| spellingShingle | Dennis Arokoyo Ibukun P. Oyeyipo Stefan S. DU Plessis Novel N. Chegou Yapo G. Aboua Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract Journal of Kerman University of Medical Sciences male reproduction streptozotocin diabetes basella alba aqueous extract |
| title | Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract |
| title_full | Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract |
| title_fullStr | Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract |
| title_full_unstemmed | Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract |
| title_short | Reproductive Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats: Beneficial Role of Basella Alba Aqueous Leave Extract |
| title_sort | reproductive parameters in streptozotocin induced diabetic male wistar rats beneficial role of basella alba aqueous leave extract |
| topic | male reproduction streptozotocin diabetes basella alba aqueous extract |
| url | https://jkmu.kmu.ac.ir/article_59761_0e9de7e191ca704214299d5835a73c2c.pdf |
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