Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions
Even if the ocean represents a large part of Earth’s surface, only a few studies describe marine-derived fungi compared to their terrestrial homologues. In this ecosystem, marine-derived fungi have had to adapt to the salinity and to the plant biomass composition. This articles studies the growth of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/9/461 |
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| author | Wissal Ben Ali David Navarro Abhishek Kumar Elodie Drula Annick Turbé-Doan Lydie Oliveira Correia Stéphanie Baumberger Emmanuel Bertrand Craig B. Faulds Bernard Henrissat Giuliano Sciara Tahar Mechichi Eric Record |
| author_facet | Wissal Ben Ali David Navarro Abhishek Kumar Elodie Drula Annick Turbé-Doan Lydie Oliveira Correia Stéphanie Baumberger Emmanuel Bertrand Craig B. Faulds Bernard Henrissat Giuliano Sciara Tahar Mechichi Eric Record |
| author_sort | Wissal Ben Ali |
| collection | DOAJ |
| description | Even if the ocean represents a large part of Earth’s surface, only a few studies describe marine-derived fungi compared to their terrestrial homologues. In this ecosystem, marine-derived fungi have had to adapt to the salinity and to the plant biomass composition. This articles studies the growth of five marine isolates and the tuning of lignocellulolytic activities under different conditions, including the salinity. A de novo transcriptome sequencing and assembly were used in combination with a proteomic approach to characterize the Carbohydrate Active Enzymes (CAZy) repertoire of one of these strains. Following these approaches, <i>Stemphylium lucomagnoense</i> was selected for its adapted growth on xylan in saline conditions, its high xylanase activity, and its improved laccase activities in seagrass-containing cultures with salt. De novo transcriptome sequencing and assembly indicated the presence of 51 putative lignocellulolytic enzymes. Its secretome composition was studied in detail when the fungus was grown on either a terrestrial or a marine substrate, under saline and non-saline conditions. Proteomic analysis of the four <i>S. lucomagnoense</i> secretomes revealed a minimal suite of extracellular enzymes for plant biomass degradation and highlighted potential enzyme targets to be further studied for their adaptation to salts and for potential biotechnological applications. |
| first_indexed | 2024-03-10T16:28:08Z |
| format | Article |
| id | doaj.art-fec7da117f3f4e49b8d636c31c64c2d3 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T16:28:08Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-fec7da117f3f4e49b8d636c31c64c2d32023-11-20T13:05:12ZengMDPI AGMarine Drugs1660-33972020-09-0118946110.3390/md18090461Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline ConditionsWissal Ben Ali0David Navarro1Abhishek Kumar2Elodie Drula3Annick Turbé-Doan4Lydie Oliveira Correia5Stéphanie Baumberger6Emmanuel Bertrand7Craig B. Faulds8Bernard Henrissat9Giuliano Sciara10Tahar Mechichi11Eric Record12Biodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceAgroParisTech, Micalis Institute, PAPPSO, Université Paris-Saclay, INRAE, 78350 Jouy-en-Josas, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, CNRS, Université Paris-Saclay, 78000 Versailles, FranceBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceArchitecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique, Aix-Marseille Université, 13288 Marseille, FranceBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceLaboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Sfax 3029, TunisiaBiodiversité et Biotechnologie Fongiques, Aix-Marseille Université, INRAE, UMR1163, 13288 Marseille, FranceEven if the ocean represents a large part of Earth’s surface, only a few studies describe marine-derived fungi compared to their terrestrial homologues. In this ecosystem, marine-derived fungi have had to adapt to the salinity and to the plant biomass composition. This articles studies the growth of five marine isolates and the tuning of lignocellulolytic activities under different conditions, including the salinity. A de novo transcriptome sequencing and assembly were used in combination with a proteomic approach to characterize the Carbohydrate Active Enzymes (CAZy) repertoire of one of these strains. Following these approaches, <i>Stemphylium lucomagnoense</i> was selected for its adapted growth on xylan in saline conditions, its high xylanase activity, and its improved laccase activities in seagrass-containing cultures with salt. De novo transcriptome sequencing and assembly indicated the presence of 51 putative lignocellulolytic enzymes. Its secretome composition was studied in detail when the fungus was grown on either a terrestrial or a marine substrate, under saline and non-saline conditions. Proteomic analysis of the four <i>S. lucomagnoense</i> secretomes revealed a minimal suite of extracellular enzymes for plant biomass degradation and highlighted potential enzyme targets to be further studied for their adaptation to salts and for potential biotechnological applications.https://www.mdpi.com/1660-3397/18/9/461marine-derived fungus<i>Stemphylium lucomagnoense</i>saline adaptationlignocellulose-degrading enzymessecretome |
| spellingShingle | Wissal Ben Ali David Navarro Abhishek Kumar Elodie Drula Annick Turbé-Doan Lydie Oliveira Correia Stéphanie Baumberger Emmanuel Bertrand Craig B. Faulds Bernard Henrissat Giuliano Sciara Tahar Mechichi Eric Record Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions Marine Drugs marine-derived fungus <i>Stemphylium lucomagnoense</i> saline adaptation lignocellulose-degrading enzymes secretome |
| title | Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions |
| title_full | Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions |
| title_fullStr | Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions |
| title_full_unstemmed | Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions |
| title_short | Characterization of the CAZy Repertoire from the Marine-Derived Fungus <i>Stemphylium lucomagnoense</i> in Relation to Saline Conditions |
| title_sort | characterization of the cazy repertoire from the marine derived fungus i stemphylium lucomagnoense i in relation to saline conditions |
| topic | marine-derived fungus <i>Stemphylium lucomagnoense</i> saline adaptation lignocellulose-degrading enzymes secretome |
| url | https://www.mdpi.com/1660-3397/18/9/461 |
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