Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i>
<i>Coccomyxa onubensis</i> (<i>C. onubensis</i>) is an acidotolerant microalga isolated from Tinto River (Huelva), which contains high levels of metal cations in solution, mainly Fe (II) and (III), and Cu (II). Fe is more bioavailable at low pH, mainly because Fe (II) and Fe...
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| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/3/610 |
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| author | María Robles Rafael Torronteras Carol Ostojic Cinta Oria María Cuaresma Inés Garbayo Francisco Navarro Carlos Vílchez |
| author_facet | María Robles Rafael Torronteras Carol Ostojic Cinta Oria María Cuaresma Inés Garbayo Francisco Navarro Carlos Vílchez |
| author_sort | María Robles |
| collection | DOAJ |
| description | <i>Coccomyxa onubensis</i> (<i>C. onubensis</i>) is an acidotolerant microalga isolated from Tinto River (Huelva), which contains high levels of metal cations in solution, mainly Fe (II) and (III), and Cu (II). Fe is more bioavailable at low pH, mainly because Fe (II) and Fe (III) are far more soluble, especially Fe (III). For this reason, this study aims to evaluate both physiological and biochemical responses of <i>C. onubensis</i> when subjected to Fe (III)-induced stress. Changes in growth, photosynthetic viability and antioxidant responses to the induced oxidative stress were determined. The results obtained suggest that the addition of moderate Fe (III) levels to <i>C. onubensis</i> cultures results in improved growth and photosynthetic viability. Increases in the intracellular levels of the enzyme superoxide dismutase (SOD) and flavonoids, used as antioxidant response biomarkers, a point at Fe (III)-mediated oxidative stress induction. The apparent decrease in the content of other phenolic molecules and polyunsaturated fatty acids might be understood as a sign of antioxidant molecules' involvement in reactive oxygen species (ROS) scavenging. In conclusion, a noticeable antioxidant capacity displayed by <i>C. onubensis</i> allows the use of moderate Fe (III) levels to trigger the accumulation of valuable antioxidant molecules, allowing the production of cell extracts with potential anti-inflammatory activity. |
| first_indexed | 2024-03-11T07:01:06Z |
| format | Article |
| id | doaj.art-fecbfec763dd48e0a55cc1308fa35156 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T07:01:06Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-fecbfec763dd48e0a55cc1308fa351562023-11-17T09:17:11ZengMDPI AGAntioxidants2076-39212023-03-0112361010.3390/antiox12030610Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i>María Robles0Rafael Torronteras1Carol Ostojic2Cinta Oria3María Cuaresma4Inés Garbayo5Francisco Navarro6Carlos Vílchez7Algal Biotechnology, CIDERTA-RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainBiology and Environmental Analysis, RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainAlgal Biotechnology, CIDERTA-RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainAlgal Biotechnology, CIDERTA-RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainAlgal Biotechnology, CIDERTA-RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainAlgal Biotechnology, CIDERTA-RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainCell Alterations by Exogenous Agents, RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, SpainAlgal Biotechnology, CIDERTA-RENSMA, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, Spain<i>Coccomyxa onubensis</i> (<i>C. onubensis</i>) is an acidotolerant microalga isolated from Tinto River (Huelva), which contains high levels of metal cations in solution, mainly Fe (II) and (III), and Cu (II). Fe is more bioavailable at low pH, mainly because Fe (II) and Fe (III) are far more soluble, especially Fe (III). For this reason, this study aims to evaluate both physiological and biochemical responses of <i>C. onubensis</i> when subjected to Fe (III)-induced stress. Changes in growth, photosynthetic viability and antioxidant responses to the induced oxidative stress were determined. The results obtained suggest that the addition of moderate Fe (III) levels to <i>C. onubensis</i> cultures results in improved growth and photosynthetic viability. Increases in the intracellular levels of the enzyme superoxide dismutase (SOD) and flavonoids, used as antioxidant response biomarkers, a point at Fe (III)-mediated oxidative stress induction. The apparent decrease in the content of other phenolic molecules and polyunsaturated fatty acids might be understood as a sign of antioxidant molecules' involvement in reactive oxygen species (ROS) scavenging. In conclusion, a noticeable antioxidant capacity displayed by <i>C. onubensis</i> allows the use of moderate Fe (III) levels to trigger the accumulation of valuable antioxidant molecules, allowing the production of cell extracts with potential anti-inflammatory activity.https://www.mdpi.com/2076-3921/12/3/610antioxidantsoxidative stressFereactive oxygen speciesphotosynthesis |
| spellingShingle | María Robles Rafael Torronteras Carol Ostojic Cinta Oria María Cuaresma Inés Garbayo Francisco Navarro Carlos Vílchez Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i> Antioxidants antioxidants oxidative stress Fe reactive oxygen species photosynthesis |
| title | Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i> |
| title_full | Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i> |
| title_fullStr | Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i> |
| title_full_unstemmed | Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i> |
| title_short | Fe (III)-Mediated Antioxidant Response of the Acidotolerant Microalga <i>Coccomyxa onubensis</i> |
| title_sort | fe iii mediated antioxidant response of the acidotolerant microalga i coccomyxa onubensis i |
| topic | antioxidants oxidative stress Fe reactive oxygen species photosynthesis |
| url | https://www.mdpi.com/2076-3921/12/3/610 |
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