Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power
This paper focuses on determination of the influence of electrochemically active microorganisms on the transmission of electrons from the respiratory enzymes to the electrode and assembling of exoelectrogens to the simulated wastewater medium. In this study, the total of eight microorganisms w...
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| Format: | Article |
| Language: | English |
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International Association of Physical Chemists (IAPC)
2021-03-01
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| Series: | Journal of Electrochemical Science and Engineering |
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| Online Access: | https://pub.iapchem.org/ojs/index.php/JESE/article/view/924 |
| _version_ | 1818881892308484096 |
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| author | Payel Choudhury Biswanath Bhunia Tarun Kanti Bandyopadhyaya |
| author_facet | Payel Choudhury Biswanath Bhunia Tarun Kanti Bandyopadhyaya |
| author_sort | Payel Choudhury |
| collection | DOAJ |
| description | This paper focuses on determination of the influence of electrochemically active microorganisms on the transmission of electrons from the respiratory enzymes to the electrode and assembling of exoelectrogens to the simulated wastewater medium. In this study, the total of eight microorganisms were experimentally tested to exhibit growth and high iron-reducing ability in the absence of mediators. A major connection was observed between the growth and iron-reduction ability of the microorganism. The growth and iron-reduction ability were monitored experimentally over time. Based on output data, the screening was done among eight different microorganisms, where Escherichia coli -K-12 was chosen as the most potent microorganism for its wide application in a microbial fuel cell (MFC). In the present study, various biochemical process factors were optimized statistically using Taguchi methodology for the rapid development of growth and iron-reducing assay conditions. The design of various experimental trials was carried out using five process factors at three levels with orthogonal arrays (OA) layout of L18. Five process factors, including quantity of lactose, volume of trace element solution, inoculum percentage, pH, and temperature, were taken into consideration as imperative process factors and optimized for evaluation of growth of bacteria and iron reduction ability. The larger-is-best signal to noise (S/N) ratio, together with analysis of variance ANOVA, were used during optimization. Anticipated results demonstrated that the enhanced bacterial growth of 124.50 % and iron reduction ability of 112.6 % can be achieved with 8 g/L of lactose, 2 ml of trace element solution, 4 % (v/v) of inoculum, pH 7, and temperature of 35 oC. Furthermore, the growth and iron reduction time profiles of Escherichia coli-K12 were performed to determine its feasibility in MFC. Open circuit voltage of 0.555 V was obtained over batch study on a single chamber microbial fuel cell (SCMFC). |
| first_indexed | 2024-12-19T15:09:05Z |
| format | Article |
| id | doaj.art-fecdac880f1440fbbcf8edb69c210b45 |
| institution | Directory Open Access Journal |
| issn | 1847-9286 |
| language | English |
| last_indexed | 2024-12-19T15:09:05Z |
| publishDate | 2021-03-01 |
| publisher | International Association of Physical Chemists (IAPC) |
| record_format | Article |
| series | Journal of Electrochemical Science and Engineering |
| spelling | doaj.art-fecdac880f1440fbbcf8edb69c210b452022-12-21T20:16:22ZengInternational Association of Physical Chemists (IAPC)Journal of Electrochemical Science and Engineering1847-92862021-03-0110.5599/jese.924Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of powerPayel Choudhury0Biswanath Bhunia1Tarun Kanti Bandyopadhyaya2Department of Electrical Engineering, National Institute of Technology Agartala, Agartala-799046, IndiaDepartment of BioEngineering, National Institute of Technology Agartala, Agartala-799046, IndiaDepartment of Chemical Engineering, National Institute of Technology Agartala, Agartala-799046, IndiaThis paper focuses on determination of the influence of electrochemically active microorganisms on the transmission of electrons from the respiratory enzymes to the electrode and assembling of exoelectrogens to the simulated wastewater medium. In this study, the total of eight microorganisms were experimentally tested to exhibit growth and high iron-reducing ability in the absence of mediators. A major connection was observed between the growth and iron-reduction ability of the microorganism. The growth and iron-reduction ability were monitored experimentally over time. Based on output data, the screening was done among eight different microorganisms, where Escherichia coli -K-12 was chosen as the most potent microorganism for its wide application in a microbial fuel cell (MFC). In the present study, various biochemical process factors were optimized statistically using Taguchi methodology for the rapid development of growth and iron-reducing assay conditions. The design of various experimental trials was carried out using five process factors at three levels with orthogonal arrays (OA) layout of L18. Five process factors, including quantity of lactose, volume of trace element solution, inoculum percentage, pH, and temperature, were taken into consideration as imperative process factors and optimized for evaluation of growth of bacteria and iron reduction ability. The larger-is-best signal to noise (S/N) ratio, together with analysis of variance ANOVA, were used during optimization. Anticipated results demonstrated that the enhanced bacterial growth of 124.50 % and iron reduction ability of 112.6 % can be achieved with 8 g/L of lactose, 2 ml of trace element solution, 4 % (v/v) of inoculum, pH 7, and temperature of 35 oC. Furthermore, the growth and iron reduction time profiles of Escherichia coli-K12 were performed to determine its feasibility in MFC. Open circuit voltage of 0.555 V was obtained over batch study on a single chamber microbial fuel cell (SCMFC).https://pub.iapchem.org/ojs/index.php/JESE/article/view/924bioelectricityexoelectrogenic bacteriabacterial growthiron-reducing abilityTaguchi optimizationbatch operation |
| spellingShingle | Payel Choudhury Biswanath Bhunia Tarun Kanti Bandyopadhyaya Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power Journal of Electrochemical Science and Engineering bioelectricity exoelectrogenic bacteria bacterial growth iron-reducing ability Taguchi optimization batch operation |
| title | Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power |
| title_full | Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power |
| title_fullStr | Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power |
| title_full_unstemmed | Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power |
| title_short | Screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power |
| title_sort | screening technique on the selection of potent microorganisms for operation in microbial fuel cell for generation of power |
| topic | bioelectricity exoelectrogenic bacteria bacterial growth iron-reducing ability Taguchi optimization batch operation |
| url | https://pub.iapchem.org/ojs/index.php/JESE/article/view/924 |
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