Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase
Abstract Background The present study was aimed to evaluate the molecular level anticaries effect of different medicinal plants against Streptococcus mutans (S.mutans) glucosyltransferases (gtf). Methods A total of six natural sources named as Terminalia chebula (T.chebula), Psidium guajava (P.guaja...
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-019-2608-3 |
| _version_ | 1811292639684722688 |
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| author | Kiranmai Mandava Uma Rajeswari Batchu Shravya Kakulavaram Shulamithi Repally Ishwarya Chennuri Srinivas Bedarakota Namratha Sunkara |
| author_facet | Kiranmai Mandava Uma Rajeswari Batchu Shravya Kakulavaram Shulamithi Repally Ishwarya Chennuri Srinivas Bedarakota Namratha Sunkara |
| author_sort | Kiranmai Mandava |
| collection | DOAJ |
| description | Abstract Background The present study was aimed to evaluate the molecular level anticaries effect of different medicinal plants against Streptococcus mutans (S.mutans) glucosyltransferases (gtf). Methods A total of six natural sources named as Terminalia chebula (T.chebula), Psidium guajava (P.guajava), Azadirachta indica (A.indica) and Pongamia pinnata (P.pinnata); two essential oils, clove (Syzygium aromaticum) and peppermint oil (Mentha piperita) were selected as test samples. Hydroalcoholic plant extracts and essential oils were examined for their inhibitory potential on gtf isolated from S.mutans. Polyherbal mouth wash was prepared and its effect on gtf activity was compared with commercial chlorhexidine mouth wash (5%w/v). Enzyme kinetic study was carried out in order to explore the molecular mechanism of enzyme action. Results Out of six natural sources tested, A.indica has shown maximum inhibitory effect of 91.647% on gtf and T.chebula has shown IC50 of 1.091 mg/ml which is significant when compared to standard chlorhexidine. From the final result of kinetic analysis it was found that T.chebula, P.guajava and P.pinnata have show uncompetitive inhibition where as A.indica has shown non-competitive inhibition. Surprisingly, both essential oils have shown allosteric inhibition (sigmoidal response). The polyherbal moutwash has shown significant inhibitory potential on gtf (95.936%) when compared to commercial chlorhexidine mouthwash (p < 0.05). Conclusion All the tested samples have shown considerable gtf inhibitory action. Moreover polyherbal mouth wash has shown promising noncompetitive inhibitory activity against gtf and it could be the future formulation to combat dental caries. |
| first_indexed | 2024-04-13T04:48:24Z |
| format | Article |
| id | doaj.art-fecdbe6e24834d1d8139473d6814d71c |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-04-13T04:48:24Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-fecdbe6e24834d1d8139473d6814d71c2022-12-22T03:01:46ZengBMCBMC Complementary and Alternative Medicine1472-68822019-08-011911810.1186/s12906-019-2608-3Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferaseKiranmai Mandava0Uma Rajeswari Batchu1Shravya Kakulavaram2Shulamithi Repally3Ishwarya Chennuri4Srinivas Bedarakota5Namratha Sunkara6Department of Pharmaceutical Chemistry, Bharat Institute of TechnologyDepartment of Pharmaceutical Biotechnology, Bharat Institute of TechnologyDepartment of Pharmaceutical Chemistry, Bharat Institute of TechnologyDepartment of Pharmaceutical Chemistry, Bharat Institute of TechnologyDepartment of Pharmaceutical Chemistry, Bharat Institute of TechnologyDepartment of Pharmaceutical Chemistry, Bharat Institute of TechnologyDepartment of Pharmaceutical Chemistry, Bharat Institute of TechnologyAbstract Background The present study was aimed to evaluate the molecular level anticaries effect of different medicinal plants against Streptococcus mutans (S.mutans) glucosyltransferases (gtf). Methods A total of six natural sources named as Terminalia chebula (T.chebula), Psidium guajava (P.guajava), Azadirachta indica (A.indica) and Pongamia pinnata (P.pinnata); two essential oils, clove (Syzygium aromaticum) and peppermint oil (Mentha piperita) were selected as test samples. Hydroalcoholic plant extracts and essential oils were examined for their inhibitory potential on gtf isolated from S.mutans. Polyherbal mouth wash was prepared and its effect on gtf activity was compared with commercial chlorhexidine mouth wash (5%w/v). Enzyme kinetic study was carried out in order to explore the molecular mechanism of enzyme action. Results Out of six natural sources tested, A.indica has shown maximum inhibitory effect of 91.647% on gtf and T.chebula has shown IC50 of 1.091 mg/ml which is significant when compared to standard chlorhexidine. From the final result of kinetic analysis it was found that T.chebula, P.guajava and P.pinnata have show uncompetitive inhibition where as A.indica has shown non-competitive inhibition. Surprisingly, both essential oils have shown allosteric inhibition (sigmoidal response). The polyherbal moutwash has shown significant inhibitory potential on gtf (95.936%) when compared to commercial chlorhexidine mouthwash (p < 0.05). Conclusion All the tested samples have shown considerable gtf inhibitory action. Moreover polyherbal mouth wash has shown promising noncompetitive inhibitory activity against gtf and it could be the future formulation to combat dental caries.http://link.springer.com/article/10.1186/s12906-019-2608-3S.mutansGlucosyltransferaseAzadirachta indicaTerminalia chebulaPolyherbal mouth washAnticaries agent |
| spellingShingle | Kiranmai Mandava Uma Rajeswari Batchu Shravya Kakulavaram Shulamithi Repally Ishwarya Chennuri Srinivas Bedarakota Namratha Sunkara Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase BMC Complementary and Alternative Medicine S.mutans Glucosyltransferase Azadirachta indica Terminalia chebula Polyherbal mouth wash Anticaries agent |
| title | Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase |
| title_full | Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase |
| title_fullStr | Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase |
| title_full_unstemmed | Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase |
| title_short | Design and study of anticaries effect of different medicinal plants against S.mutans glucosyltransferase |
| title_sort | design and study of anticaries effect of different medicinal plants against s mutans glucosyltransferase |
| topic | S.mutans Glucosyltransferase Azadirachta indica Terminalia chebula Polyherbal mouth wash Anticaries agent |
| url | http://link.springer.com/article/10.1186/s12906-019-2608-3 |
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