The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model
This study assessed the effect of cow milk (CM) and sheep milk (SM) consumption on the micro-structure, mechanical function, and mineral composition of rat femora in a male weanling rat model. Male weanling rats were fed a basal diet with a 50% reduction in calcium and phosphorus content (low Ca/P-d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/8/1070 |
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| author | Keegan Burrow Wayne Young Niels Hammer Sarah Safavi Mario Scholze Michelle McConnell Alan Carne David Barr Malcolm Reid Alaa El-Din Bekhit |
| author_facet | Keegan Burrow Wayne Young Niels Hammer Sarah Safavi Mario Scholze Michelle McConnell Alan Carne David Barr Malcolm Reid Alaa El-Din Bekhit |
| author_sort | Keegan Burrow |
| collection | DOAJ |
| description | This study assessed the effect of cow milk (CM) and sheep milk (SM) consumption on the micro-structure, mechanical function, and mineral composition of rat femora in a male weanling rat model. Male weanling rats were fed a basal diet with a 50% reduction in calcium and phosphorus content (low Ca/P-diet) supplemented with either SM or CM. Rats were fed for 28 days, after which the femora were harvested and stored. The femora were analyzed by μ-CT, three-point bending, and inductively coupled plasma–mass spectrometry (ICP-MS). The addition of either milk to the low Ca/P-diet significantly increased (<i>p</i> < 0.05) trabecular bone volume, trabecular bone surface density, trabecular number, cortical bone volume, and maximum force, when compared to rats that consumed only the low Ca/P-diet. The consumption of either milk resulted in a significant decrease (<i>p</i> < 0.05) in trabecular pattern factor, and cortical bone surface to volume ratio when compared to rats that consumed only the low Ca/P-diet. The results were achieved with a lower consumption of SM compared to that of CM (<i>p</i> < 0.05). This work indicates that SM and CM can help overcome the effects on bone of a restriction in calcium and phosphorus intake. |
| first_indexed | 2024-03-10T17:49:27Z |
| format | Article |
| id | doaj.art-fecf8e6715fa4c2b895b2d7994bc770d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T17:49:27Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-fecf8e6715fa4c2b895b2d7994bc770d2023-11-20T09:24:55ZengMDPI AGFoods2304-81582020-08-0198107010.3390/foods9081070The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat ModelKeegan Burrow0Wayne Young1Niels Hammer2Sarah Safavi3Mario Scholze4Michelle McConnell5Alan Carne6David Barr7Malcolm Reid8Alaa El-Din Bekhit9Department of Wine, Food and Molecular Biosciences, Lincoln University, PO Box 85084, Lincoln 7647, New ZealandAgResearch Ltd, Grasslands Research Centre, Private Bag 11008, Manawatu Mail Centre, Palmerston North 4442, New ZealandDepartment of Macroscopic and Clinical Anatomy, Medical University of Graz, 8010 Graz, AustriaDepartment of Anatomy, University of Otago, PO Box 56, Dunedin 9054, New ZealandInstitute of Materials Science and Engineering, Chemnitz University of Technology, Straße der Nationen, 62, 09111 Chemnitz, GermanyDepartment of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New ZealandDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New ZealandDepartment of Chemistry, University of Otago, PO Box 56, Dunedin 9054, New ZealandDepartment of Chemistry, University of Otago, PO Box 56, Dunedin 9054, New ZealandDepartment of Food Science, University of Otago, PO Box 56, Dunedin 9054, New ZealandThis study assessed the effect of cow milk (CM) and sheep milk (SM) consumption on the micro-structure, mechanical function, and mineral composition of rat femora in a male weanling rat model. Male weanling rats were fed a basal diet with a 50% reduction in calcium and phosphorus content (low Ca/P-diet) supplemented with either SM or CM. Rats were fed for 28 days, after which the femora were harvested and stored. The femora were analyzed by μ-CT, three-point bending, and inductively coupled plasma–mass spectrometry (ICP-MS). The addition of either milk to the low Ca/P-diet significantly increased (<i>p</i> < 0.05) trabecular bone volume, trabecular bone surface density, trabecular number, cortical bone volume, and maximum force, when compared to rats that consumed only the low Ca/P-diet. The consumption of either milk resulted in a significant decrease (<i>p</i> < 0.05) in trabecular pattern factor, and cortical bone surface to volume ratio when compared to rats that consumed only the low Ca/P-diet. The results were achieved with a lower consumption of SM compared to that of CM (<i>p</i> < 0.05). This work indicates that SM and CM can help overcome the effects on bone of a restriction in calcium and phosphorus intake.https://www.mdpi.com/2304-8158/9/8/1070bonedeficient dietdairymineralsmicro-computed tomographyinductively coupled plasma–mass spectrometry |
| spellingShingle | Keegan Burrow Wayne Young Niels Hammer Sarah Safavi Mario Scholze Michelle McConnell Alan Carne David Barr Malcolm Reid Alaa El-Din Bekhit The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model Foods bone deficient diet dairy minerals micro-computed tomography inductively coupled plasma–mass spectrometry |
| title | The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model |
| title_full | The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model |
| title_fullStr | The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model |
| title_full_unstemmed | The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model |
| title_short | The Effect of the Supplementation of a Diet Low in Calcium and Phosphorus with Either Sheep Milk or Cow Milk on the Physical and Mechanical Characteristics of Bone using A Rat Model |
| title_sort | effect of the supplementation of a diet low in calcium and phosphorus with either sheep milk or cow milk on the physical and mechanical characteristics of bone using a rat model |
| topic | bone deficient diet dairy minerals micro-computed tomography inductively coupled plasma–mass spectrometry |
| url | https://www.mdpi.com/2304-8158/9/8/1070 |
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