Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts
Apocynin is a potent and selective inhibitor of the NADPH oxidase-dependent production of ROS by stimulated human PMNs. Apocynin was isolated by activity-guided isolation from Picrorhiza kurroa , and in the years following its discovery it has been used in many laboratories around the world. Reactiv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2011-05-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X1100900210 |
| _version_ | 1819205241365594112 |
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| author | R. Di Paola E. Mazzon I. Paterniti D. Impellizzeri P. Bramanti S. Cuzzocrea |
| author_facet | R. Di Paola E. Mazzon I. Paterniti D. Impellizzeri P. Bramanti S. Cuzzocrea |
| author_sort | R. Di Paola |
| collection | DOAJ |
| description | Apocynin is a potent and selective inhibitor of the NADPH oxidase-dependent production of ROS by stimulated human PMNs. Apocynin was isolated by activity-guided isolation from Picrorhiza kurroa , and in the years following its discovery it has been used in many laboratories around the world. Reactive oxygen species (ROS) production by activated polymorphonuclear neutrophils (PMNs) plays an important role in many cardiovascular disease states, including myocardial ischemia reperfusion injury. The purpose of this study is to examine the beneficial effects of apocynin on myocardial ischemia reperfusion injury. Myocardial I/R injury was caused by clamping the left anterior descending (LAD) coronary artery for 20 min followed by release of the clamp allowing reperfusion for 1 h. Administration of apocynin i.p. (5mg/kg i.p. 10%DMSO) 15 min after ischemia significantly reduced the: 1) histological evidence of myocardial injury; 2) pro-inflammatory cytokines (TNF-α, IL-1β); 3) adhesion molecules (ICAM-1, P-Selectin); 4) nitrotyrosine formation; 5) NF-kB expression; 6) PAR formation; and 7) apoptosis (Bax, Bcl-2, Fas-L and tunel). Based on these findings we propose that apocynin would be useful in the treatment of various ischemia and reperfusion diseases. |
| first_indexed | 2024-12-23T04:48:34Z |
| format | Article |
| id | doaj.art-fed0009a18784f898c799d42729640e1 |
| institution | Directory Open Access Journal |
| issn | 1721-727X |
| language | English |
| last_indexed | 2024-12-23T04:48:34Z |
| publishDate | 2011-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-fed0009a18784f898c799d42729640e12022-12-21T17:59:32ZengSAGE PublishingEuropean Journal of Inflammation1721-727X2011-05-01910.1177/1721727X1100900210Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat HeartsR. Di Paola0E. Mazzon1I. Paterniti2D. Impellizzeri3P. Bramanti4S. Cuzzocrea5 IRCCS Centro Neurolesi “Bonino-Pulejo”, Messina IRCCS Centro Neurolesi “Bonino-Pulejo”, Messina Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Italy Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Italy IRCCS Centro Neurolesi “Bonino-Pulejo”, Messina Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, ItalyApocynin is a potent and selective inhibitor of the NADPH oxidase-dependent production of ROS by stimulated human PMNs. Apocynin was isolated by activity-guided isolation from Picrorhiza kurroa , and in the years following its discovery it has been used in many laboratories around the world. Reactive oxygen species (ROS) production by activated polymorphonuclear neutrophils (PMNs) plays an important role in many cardiovascular disease states, including myocardial ischemia reperfusion injury. The purpose of this study is to examine the beneficial effects of apocynin on myocardial ischemia reperfusion injury. Myocardial I/R injury was caused by clamping the left anterior descending (LAD) coronary artery for 20 min followed by release of the clamp allowing reperfusion for 1 h. Administration of apocynin i.p. (5mg/kg i.p. 10%DMSO) 15 min after ischemia significantly reduced the: 1) histological evidence of myocardial injury; 2) pro-inflammatory cytokines (TNF-α, IL-1β); 3) adhesion molecules (ICAM-1, P-Selectin); 4) nitrotyrosine formation; 5) NF-kB expression; 6) PAR formation; and 7) apoptosis (Bax, Bcl-2, Fas-L and tunel). Based on these findings we propose that apocynin would be useful in the treatment of various ischemia and reperfusion diseases.https://doi.org/10.1177/1721727X1100900210 |
| spellingShingle | R. Di Paola E. Mazzon I. Paterniti D. Impellizzeri P. Bramanti S. Cuzzocrea Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts European Journal of Inflammation |
| title | Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts |
| title_full | Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts |
| title_fullStr | Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts |
| title_full_unstemmed | Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts |
| title_short | Apocynin, a Plant-Derived Drug, Might Be Useful in the Treatment of Myocardial Ischemia Reperfusion Injury in Rat Hearts |
| title_sort | apocynin a plant derived drug might be useful in the treatment of myocardial ischemia reperfusion injury in rat hearts |
| url | https://doi.org/10.1177/1721727X1100900210 |
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