Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions
Morphometric procedures were used to examine peroxisome number and di-stribution in pea (Pisum sativum L.) root nodules under NaCl (50 mM) or HgCl2 (7.3 µM) treatment. Peroxisomes were visualized cytochemically in meristem, invasion zone and prefixing zone of pea root nodules by catalase (EC 1.11.1....
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2011-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/502 |
| _version_ | 1819330518041231360 |
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| author | Wojciech Borucki |
| author_facet | Wojciech Borucki |
| author_sort | Wojciech Borucki |
| collection | DOAJ |
| description | Morphometric procedures were used to examine peroxisome number and di-stribution in pea (Pisum sativum L.) root nodules under NaCl (50 mM) or HgCl2 (7.3 µM) treatment. Peroxisomes were visualized cytochemically in meristem, invasion zone and prefixing zone of pea root nodules by catalase (EC 1.11.1.6) activity. The observations using light and electron microscopy revealed that the peroxisomes were predominantly spherical in shape and showed catalase activity. In nitrogen fixation zone, catalase active peroxisomes were observed occasionally. Bacteroids of nitrogen fixing zone showed enhanced cata-lase activity probably as a response to higher level of oxidative stress. Fluorescence microscopy investigations revealed enhanced level of (homo)glutathione in prefixing and nitrogen-fixing zone of NaCl- and Hg2+treated nodules, which served as an indicator of antioxidative response. Morphometric measurements revealed that during differentiation of meristematic cells into central tissue (bacteroidal tissue) cells an increase in peroxisome number was observed in unstressed nodules. Peroxisomes located in meristem, invasion zone and prefixing zone of NaCl- and Hg2+-treated nodules outnumbered that in control nodules. A substantial enlargement of peroxisome profiles was detected in NaCl- and Hg2+treated nodules. Peroxisome divisions observed in meristematic and infection thread penetration zone were responsible for an increase in peroxisome number. |
| first_indexed | 2024-12-24T13:59:48Z |
| format | Article |
| id | doaj.art-fed430d3ba8943adb2978a13a29f7abd |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-24T13:59:48Z |
| publishDate | 2011-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-fed430d3ba8943adb2978a13a29f7abd2022-12-21T16:52:30ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-0176428729810.5586/asbp.2007.032309Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditionsWojciech Borucki0Agricultural University of WarsawMorphometric procedures were used to examine peroxisome number and di-stribution in pea (Pisum sativum L.) root nodules under NaCl (50 mM) or HgCl2 (7.3 µM) treatment. Peroxisomes were visualized cytochemically in meristem, invasion zone and prefixing zone of pea root nodules by catalase (EC 1.11.1.6) activity. The observations using light and electron microscopy revealed that the peroxisomes were predominantly spherical in shape and showed catalase activity. In nitrogen fixation zone, catalase active peroxisomes were observed occasionally. Bacteroids of nitrogen fixing zone showed enhanced cata-lase activity probably as a response to higher level of oxidative stress. Fluorescence microscopy investigations revealed enhanced level of (homo)glutathione in prefixing and nitrogen-fixing zone of NaCl- and Hg2+treated nodules, which served as an indicator of antioxidative response. Morphometric measurements revealed that during differentiation of meristematic cells into central tissue (bacteroidal tissue) cells an increase in peroxisome number was observed in unstressed nodules. Peroxisomes located in meristem, invasion zone and prefixing zone of NaCl- and Hg2+-treated nodules outnumbered that in control nodules. A substantial enlargement of peroxisome profiles was detected in NaCl- and Hg2+treated nodules. Peroxisome divisions observed in meristematic and infection thread penetration zone were responsible for an increase in peroxisome number.https://pbsociety.org.pl/journals/index.php/asbp/article/view/502Pisum sativumperoxisome proliferationperoxisome divisioncytochemistrycatalase activitymorphometryNaClsalinitymercury treatmentroot nodules |
| spellingShingle | Wojciech Borucki Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions Acta Societatis Botanicorum Poloniae Pisum sativum peroxisome proliferation peroxisome division cytochemistry catalase activity morphometry NaCl salinity mercury treatment root nodules |
| title | Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions |
| title_full | Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions |
| title_fullStr | Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions |
| title_full_unstemmed | Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions |
| title_short | Proliferation of peroxisomes in pea root nodules - an influence of NaCI- or Hg2+- stress conditions |
| title_sort | proliferation of peroxisomes in pea root nodules an influence of naci or hg2 stress conditions |
| topic | Pisum sativum peroxisome proliferation peroxisome division cytochemistry catalase activity morphometry NaCl salinity mercury treatment root nodules |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/502 |
| work_keys_str_mv | AT wojciechborucki proliferationofperoxisomesinpearootnodulesaninfluenceofnaciorhg2stressconditions |