A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila.
A commonly used enzymatic recycling assay for pyridine nucleotides has been adapted to directly measure the NAD(+)/NADH redox ratio in Drosophila melanogaster. This method is also suitable for quantification of NADP(+) and NADPH. The addition of a coupling reaction removing acetaldehyde produced fro...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3474733?pdf=render |
| _version_ | 1818237848329912320 |
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| author | Chen-Tseh Zhu David M Rand |
| author_facet | Chen-Tseh Zhu David M Rand |
| author_sort | Chen-Tseh Zhu |
| collection | DOAJ |
| description | A commonly used enzymatic recycling assay for pyridine nucleotides has been adapted to directly measure the NAD(+)/NADH redox ratio in Drosophila melanogaster. This method is also suitable for quantification of NADP(+) and NADPH. The addition of a coupling reaction removing acetaldehyde produced from the alcohol dehydrogenase (ADH) reaction was shown to improve the linearity of NAD(H) assay. The advantages of this assay method are that it allows the determination of both NAD(+) and NADH simultaneously while keeping enzymatic degradation of pyridine nucleotides minimal and also achieving better sensitivity. This method was used to determine the redox ratio of D. melanogaster and validated substantial decrease of redox ratio during starvation. |
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| format | Article |
| id | doaj.art-fedab58572244deb9cfd132e02709629 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T12:32:17Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fedab58572244deb9cfd132e027096292022-12-22T00:24:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4758410.1371/journal.pone.0047584A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila.Chen-Tseh ZhuDavid M RandA commonly used enzymatic recycling assay for pyridine nucleotides has been adapted to directly measure the NAD(+)/NADH redox ratio in Drosophila melanogaster. This method is also suitable for quantification of NADP(+) and NADPH. The addition of a coupling reaction removing acetaldehyde produced from the alcohol dehydrogenase (ADH) reaction was shown to improve the linearity of NAD(H) assay. The advantages of this assay method are that it allows the determination of both NAD(+) and NADH simultaneously while keeping enzymatic degradation of pyridine nucleotides minimal and also achieving better sensitivity. This method was used to determine the redox ratio of D. melanogaster and validated substantial decrease of redox ratio during starvation.http://europepmc.org/articles/PMC3474733?pdf=render |
| spellingShingle | Chen-Tseh Zhu David M Rand A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila. PLoS ONE |
| title | A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila. |
| title_full | A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila. |
| title_fullStr | A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila. |
| title_full_unstemmed | A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila. |
| title_short | A hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in Drosophila. |
| title_sort | hydrazine coupled cycling assay validates the decrease in redox ratio under starvation in drosophila |
| url | http://europepmc.org/articles/PMC3474733?pdf=render |
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