Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition
In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed. The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure. These materials will be...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/9/2004 |
| _version_ | 1797554745363136512 |
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| author | Christian Enrique Garcia Garcia Félix Armando Soltero Martínez Frédéric Bossard Marguerite Rinaudo |
| author_facet | Christian Enrique Garcia Garcia Félix Armando Soltero Martínez Frédéric Bossard Marguerite Rinaudo |
| author_sort | Christian Enrique Garcia Garcia |
| collection | DOAJ |
| description | In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed. The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure. These materials will be tested for chondrocyte development in tissue engineering and wound healing applications. Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison. To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4. The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation. The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances. The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined. Acid formic/water 50/50 <i>v/v</i> is observed to be the more appropriated solvent for the carried-out procedures. |
| first_indexed | 2024-03-10T16:37:24Z |
| format | Article |
| id | doaj.art-fedeb65a4c87467a9572c58bd0d0bd97 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T16:37:24Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-fedeb65a4c87467a9572c58bd0d0bd972023-11-20T12:24:22ZengMDPI AGPolymers2073-43602020-09-01129200410.3390/polym12092004Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the CompositionChristian Enrique Garcia Garcia0Félix Armando Soltero Martínez1Frédéric Bossard2Marguerite Rinaudo3Departamento de Ingeniería Química-CUCEI. Blvd. M. García Barragán #1451, C.P., Universidad de Guadalajara, Guadalajara, Jalisco 44430, MexicoDepartamento de Ingeniería Química-CUCEI. Blvd. M. García Barragán #1451, C.P., Universidad de Guadalajara, Guadalajara, Jalisco 44430, MexicoUniversité Grenoble Alpes, CNRS, Grenoble INP, LRP, Institute of Engineering University of Grenoble Alpes, 38000 Grenoble, FranceBiomaterials Applications, 6 Rue Lesdiguières, 38000 Grenoble, FranceIn this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed. The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure. These materials will be tested for chondrocyte development in tissue engineering and wound healing applications. Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison. To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4. The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation. The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances. The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined. Acid formic/water 50/50 <i>v/v</i> is observed to be the more appropriated solvent for the carried-out procedures.https://www.mdpi.com/2073-4360/12/9/2004chitosanhyaluronanpolyelectrolyte complexcrosslinkH-bondsamide bonds |
| spellingShingle | Christian Enrique Garcia Garcia Félix Armando Soltero Martínez Frédéric Bossard Marguerite Rinaudo Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition Polymers chitosan hyaluronan polyelectrolyte complex crosslink H-bonds amide bonds |
| title | Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition |
| title_full | Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition |
| title_fullStr | Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition |
| title_full_unstemmed | Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition |
| title_short | Production of Chitosan/Hyaluronan Complex Nanofibers. Characterization and Physical Properties as a Function of the Composition |
| title_sort | production of chitosan hyaluronan complex nanofibers characterization and physical properties as a function of the composition |
| topic | chitosan hyaluronan polyelectrolyte complex crosslink H-bonds amide bonds |
| url | https://www.mdpi.com/2073-4360/12/9/2004 |
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