Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i>
A comparative study of melanin and ommochrome-containing samples, isolated from the black soldier fly (BSF) by enzymatic hydrolysis, alkaline and acid alcohol extraction or by acid hydrolysis, was carried out. Melanin was isolated both as a melanin-chitin complex and as a water-soluble melanin. Acid...
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MDPI AG
2019-08-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/9/408 |
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| author | Nina Ushakova Alexander Dontsov Natalia Sakina Alexander Bastrakov Mikhail Ostrovsky |
| author_facet | Nina Ushakova Alexander Dontsov Natalia Sakina Alexander Bastrakov Mikhail Ostrovsky |
| author_sort | Nina Ushakova |
| collection | DOAJ |
| description | A comparative study of melanin and ommochrome-containing samples, isolated from the black soldier fly (BSF) by enzymatic hydrolysis, alkaline and acid alcohol extraction or by acid hydrolysis, was carried out. Melanin was isolated both as a melanin-chitin complex and as a water-soluble melanin. Acid hydrolysis followed by delipidization yielded a more concentrated melanin sample, the electron spin resonance (ESR) signal of which was 2.6 × 10<sup>18</sup> spin/g. The ommochromes were extracted from the BSF eyes with acid methanol. The antiradical activity of BSF melanins and ommochromes was determined by the method of quenching of luminol chemiluminescence. It has been shown that delipidization of water-soluble melanin increases its antioxidant properties. A comparison of the antioxidant activity of BSF melanins and ommochromes in relation to photoinduced lipid peroxidation was carried out. The ESR characteristics of native and oxidized melanins and ommochromes were studied. It is assumed that <i>H. illucens</i> adult flies can be a useful source of natural pigments with antioxidant properties. |
| first_indexed | 2024-12-12T01:15:26Z |
| format | Article |
| id | doaj.art-fedf5bb4892341dc82bf973ab4504662 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-12T01:15:26Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-fedf5bb4892341dc82bf973ab45046622022-12-22T00:43:23ZengMDPI AGBiomolecules2218-273X2019-08-019940810.3390/biom9090408biom9090408Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i>Nina Ushakova0Alexander Dontsov1Natalia Sakina2Alexander Bastrakov3Mikhail Ostrovsky4A.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, 119071 Moscow, RussiaN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 119334 Moscow, RussiaN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 119334 Moscow, RussiaA.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, 119071 Moscow, RussiaN.M. Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 119334 Moscow, RussiaA comparative study of melanin and ommochrome-containing samples, isolated from the black soldier fly (BSF) by enzymatic hydrolysis, alkaline and acid alcohol extraction or by acid hydrolysis, was carried out. Melanin was isolated both as a melanin-chitin complex and as a water-soluble melanin. Acid hydrolysis followed by delipidization yielded a more concentrated melanin sample, the electron spin resonance (ESR) signal of which was 2.6 × 10<sup>18</sup> spin/g. The ommochromes were extracted from the BSF eyes with acid methanol. The antiradical activity of BSF melanins and ommochromes was determined by the method of quenching of luminol chemiluminescence. It has been shown that delipidization of water-soluble melanin increases its antioxidant properties. A comparison of the antioxidant activity of BSF melanins and ommochromes in relation to photoinduced lipid peroxidation was carried out. The ESR characteristics of native and oxidized melanins and ommochromes were studied. It is assumed that <i>H. illucens</i> adult flies can be a useful source of natural pigments with antioxidant properties.https://www.mdpi.com/2218-273X/9/9/408black soldier flymelaninsommochromesantioxidant activityESR |
| spellingShingle | Nina Ushakova Alexander Dontsov Natalia Sakina Alexander Bastrakov Mikhail Ostrovsky Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i> Biomolecules black soldier fly melanins ommochromes antioxidant activity ESR |
| title | Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i> |
| title_full | Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i> |
| title_fullStr | Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i> |
| title_full_unstemmed | Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i> |
| title_short | Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly <i>Hermetia illucens</i> |
| title_sort | antioxidative properties of melanins and ommochromes from black soldier fly i hermetia illucens i |
| topic | black soldier fly melanins ommochromes antioxidant activity ESR |
| url | https://www.mdpi.com/2218-273X/9/9/408 |
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