A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.

Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (DA) and the measured area (MA) for assessing fungicide growt...

Full description

Bibliographic Details
Main Authors: Katherine E Hendricks, Mary C Christman, Pamela D Roberts
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5268463?pdf=render
_version_ 1811212091173896192
author Katherine E Hendricks
Mary C Christman
Pamela D Roberts
author_facet Katherine E Hendricks
Mary C Christman
Pamela D Roberts
author_sort Katherine E Hendricks
collection DOAJ
description Fungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (DA) and the measured area (MA) for assessing fungicide growth inhibition of the ascomycete, Phyllosticta citricarpa on solid media. Both the calculated, DA and the actual measured area, MA were adequate for distinguishing significant treatment effects of fungicide on fungal growth, however MA was more sensitive at identifying significant differences between the controls and fungicide concentrations below 5 ppm.
first_indexed 2024-04-12T05:23:02Z
format Article
id doaj.art-fee0172e81ef490f8951da1c08adbc74
institution Directory Open Access Journal
issn 1932-6203
language English
last_indexed 2024-04-12T05:23:02Z
publishDate 2017-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj.art-fee0172e81ef490f8951da1c08adbc742022-12-22T03:46:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e017075510.1371/journal.pone.0170755A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.Katherine E HendricksMary C ChristmanPamela D RobertsFungal growth inhibition on solid media has been historically measured and calculated based on the average of perpendicular diameter measurements of growth on fungicide amended media. We investigated the sensitivity of the calculated area (DA) and the measured area (MA) for assessing fungicide growth inhibition of the ascomycete, Phyllosticta citricarpa on solid media. Both the calculated, DA and the actual measured area, MA were adequate for distinguishing significant treatment effects of fungicide on fungal growth, however MA was more sensitive at identifying significant differences between the controls and fungicide concentrations below 5 ppm.http://europepmc.org/articles/PMC5268463?pdf=render
spellingShingle Katherine E Hendricks
Mary C Christman
Pamela D Roberts
A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
PLoS ONE
title A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
title_full A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
title_fullStr A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
title_full_unstemmed A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
title_short A Statistical Evaluation of Methods of In-Vitro Growth Assessment for Phyllosticta citricarpa: Average Colony Diameter vs. Area.
title_sort statistical evaluation of methods of in vitro growth assessment for phyllosticta citricarpa average colony diameter vs area
url http://europepmc.org/articles/PMC5268463?pdf=render
work_keys_str_mv AT katherineehendricks astatisticalevaluationofmethodsofinvitrogrowthassessmentforphyllostictacitricarpaaveragecolonydiametervsarea
AT marycchristman astatisticalevaluationofmethodsofinvitrogrowthassessmentforphyllostictacitricarpaaveragecolonydiametervsarea
AT pameladroberts astatisticalevaluationofmethodsofinvitrogrowthassessmentforphyllostictacitricarpaaveragecolonydiametervsarea
AT katherineehendricks statisticalevaluationofmethodsofinvitrogrowthassessmentforphyllostictacitricarpaaveragecolonydiametervsarea
AT marycchristman statisticalevaluationofmethodsofinvitrogrowthassessmentforphyllostictacitricarpaaveragecolonydiametervsarea
AT pameladroberts statisticalevaluationofmethodsofinvitrogrowthassessmentforphyllostictacitricarpaaveragecolonydiametervsarea