Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i>
<i>Corynebacterium glutamicum</i> is regarded as an industrially important microbial cell factory and is widely used to produce various value-added chemicals. Because of the importance of <i>C</i>. <i>glutamicum</i> applications, current research is increasingly f...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/2/157 |
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| author | Nannan Zhao Jian Wang Aiqing Jia Ying Lin Suiping Zheng |
| author_facet | Nannan Zhao Jian Wang Aiqing Jia Ying Lin Suiping Zheng |
| author_sort | Nannan Zhao |
| collection | DOAJ |
| description | <i>Corynebacterium glutamicum</i> is regarded as an industrially important microbial cell factory and is widely used to produce various value-added chemicals. Because of the importance of <i>C</i>. <i>glutamicum</i> applications, current research is increasingly focusing on developing <i>C. glutamicum</i> synthetic biology platforms. Because of its ability to condense with adipic acid to synthesize the industrial plastic nylon-46, putrescine is an important platform compound of industrial interest. Developing a high-throughput putrescine biosensor can aid in accelerating the design–build–test cycle of cell factories (production strains) to achieve high putrescine-generating strain production in <i>C. glutamicum</i>. This study developed a putrescine-specific biosensor (pSenPuuR) in <i>C</i>. <i>glutamicum</i> using <i>Escherichia coli</i>-derived transcriptional factor PuuR. The response characteristics of the biosensor to putrescine were further improved by optimizing the genetic components of pSenPuuR, such as the response promoter, reporter protein, and promoter for controlling PuuR expression. According to the findings of the study, pSenPuuR has the potential to be used to assess putrescine production in <i>C. glutamicum</i> and is suitable for high-throughput genetic variant screening. |
| first_indexed | 2024-03-11T09:09:33Z |
| format | Article |
| id | doaj.art-fee2f14d6d3148b69a456aa646ed1438 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T09:09:33Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-fee2f14d6d3148b69a456aa646ed14382023-11-16T19:10:24ZengMDPI AGBioengineering2306-53542023-01-0110215710.3390/bioengineering10020157Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i>Nannan Zhao0Jian Wang1Aiqing Jia2Ying Lin3Suiping Zheng4Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, ChinaAnimal Husbandry and Fisheries Research Center of Guangdong Haid Group Co., Ltd., Guangzhou 511400, ChinaAnimal Husbandry and Fisheries Research Center of Guangdong Haid Group Co., Ltd., Guangzhou 511400, ChinaGuangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, ChinaGuangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China<i>Corynebacterium glutamicum</i> is regarded as an industrially important microbial cell factory and is widely used to produce various value-added chemicals. Because of the importance of <i>C</i>. <i>glutamicum</i> applications, current research is increasingly focusing on developing <i>C. glutamicum</i> synthetic biology platforms. Because of its ability to condense with adipic acid to synthesize the industrial plastic nylon-46, putrescine is an important platform compound of industrial interest. Developing a high-throughput putrescine biosensor can aid in accelerating the design–build–test cycle of cell factories (production strains) to achieve high putrescine-generating strain production in <i>C. glutamicum</i>. This study developed a putrescine-specific biosensor (pSenPuuR) in <i>C</i>. <i>glutamicum</i> using <i>Escherichia coli</i>-derived transcriptional factor PuuR. The response characteristics of the biosensor to putrescine were further improved by optimizing the genetic components of pSenPuuR, such as the response promoter, reporter protein, and promoter for controlling PuuR expression. According to the findings of the study, pSenPuuR has the potential to be used to assess putrescine production in <i>C. glutamicum</i> and is suitable for high-throughput genetic variant screening.https://www.mdpi.com/2306-5354/10/2/157<i>Corynebacterium glutamicum</i>putrescinetranscriptional factorbiosensor |
| spellingShingle | Nannan Zhao Jian Wang Aiqing Jia Ying Lin Suiping Zheng Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i> Bioengineering <i>Corynebacterium glutamicum</i> putrescine transcriptional factor biosensor |
| title | Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i> |
| title_full | Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i> |
| title_fullStr | Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i> |
| title_full_unstemmed | Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i> |
| title_short | Development of a Transcriptional Factor PuuR-Based Putrescine-Specific Biosensor in <i>Corynebacterium glutamicum</i> |
| title_sort | development of a transcriptional factor puur based putrescine specific biosensor in i corynebacterium glutamicum i |
| topic | <i>Corynebacterium glutamicum</i> putrescine transcriptional factor biosensor |
| url | https://www.mdpi.com/2306-5354/10/2/157 |
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