Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro
Osseous implantology’s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient’s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titani...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/12/2465 |
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| author | Livia Elena Sima Anca Bonciu Madalina Baciu Iulia Anghel Luminita Nicoleta Dumitrescu Laurentiu Rusen Valentina Dinca |
| author_facet | Livia Elena Sima Anca Bonciu Madalina Baciu Iulia Anghel Luminita Nicoleta Dumitrescu Laurentiu Rusen Valentina Dinca |
| author_sort | Livia Elena Sima |
| collection | DOAJ |
| description | Osseous implantology’s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient’s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titanium dental and cochlear bone-anchored hearing aid (BAHA) implants, implying the need for endowing the surface with biologically instructive properties by changing basic parameters such as surface texture. Within this context, we propose anisotropic and isotropic patterns (linear microgroove arrays, and superimposed crossline microgroove arrays, respectively) textured in zirconia substrates, as bioinstructive interfaces to guide the cytoskeletal organization of human mesenchymal stem cells (hMSCs). The designed textured micro-nano interfaces with either steep ridges and microgratings or curved edges, and nanoroughened walls obtained by direct femtosecond laser texturing are used to evaluate the hMSC response parameters and osteogenic differentiation to each topography. Our results show parallel micro line anisotropic surfaces are able to guide cell growth only for the steep surfaces, while the curved ones reduce the initial response and show the lowest osteogenic response. An improved osteogenic phenotype of hMSCs is obtained when grown onto isotropic grid/pillar-like patterns, showing an improved cell coverage and Ca/P ratio, with direct implications for BAHA prosthetic development, or other future applications in regenerating bone defects. |
| first_indexed | 2024-03-10T14:13:14Z |
| format | Article |
| id | doaj.art-fee36f2b3f1449e5b34f9f1fd98db7e9 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T14:13:14Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-fee36f2b3f1449e5b34f9f1fd98db7e92023-11-21T00:02:56ZengMDPI AGNanomaterials2079-49912020-12-011012246510.3390/nano10122465Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In VitroLivia Elena Sima0Anca Bonciu1Madalina Baciu2Iulia Anghel3Luminita Nicoleta Dumitrescu4Laurentiu Rusen5Valentina Dinca6Department of Molecular Cell Biology, Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaOtorhinolaryngology Department, MedLife Clinic, 79-81 Bvd. Tomis, 900178 Constanta, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, RomaniaOsseous implantology’s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient’s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titanium dental and cochlear bone-anchored hearing aid (BAHA) implants, implying the need for endowing the surface with biologically instructive properties by changing basic parameters such as surface texture. Within this context, we propose anisotropic and isotropic patterns (linear microgroove arrays, and superimposed crossline microgroove arrays, respectively) textured in zirconia substrates, as bioinstructive interfaces to guide the cytoskeletal organization of human mesenchymal stem cells (hMSCs). The designed textured micro-nano interfaces with either steep ridges and microgratings or curved edges, and nanoroughened walls obtained by direct femtosecond laser texturing are used to evaluate the hMSC response parameters and osteogenic differentiation to each topography. Our results show parallel micro line anisotropic surfaces are able to guide cell growth only for the steep surfaces, while the curved ones reduce the initial response and show the lowest osteogenic response. An improved osteogenic phenotype of hMSCs is obtained when grown onto isotropic grid/pillar-like patterns, showing an improved cell coverage and Ca/P ratio, with direct implications for BAHA prosthetic development, or other future applications in regenerating bone defects.https://www.mdpi.com/2079-4991/10/12/2465Zirconiamesenchymal stem cellsmicro-nano topographyosteogenic differentiationbone-anchored hearing aid (BAHA) |
| spellingShingle | Livia Elena Sima Anca Bonciu Madalina Baciu Iulia Anghel Luminita Nicoleta Dumitrescu Laurentiu Rusen Valentina Dinca Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro Nanomaterials Zirconia mesenchymal stem cells micro-nano topography osteogenic differentiation bone-anchored hearing aid (BAHA) |
| title | Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro |
| title_full | Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro |
| title_fullStr | Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro |
| title_full_unstemmed | Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro |
| title_short | Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro |
| title_sort | bioinstructive micro nanotextured zirconia ceramic interfaces for guiding and stimulating an osteogenic response in vitro |
| topic | Zirconia mesenchymal stem cells micro-nano topography osteogenic differentiation bone-anchored hearing aid (BAHA) |
| url | https://www.mdpi.com/2079-4991/10/12/2465 |
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