Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant
Abstract Worldwide wastewater treatment plants generate enormous amounts of sewage sludge, and their further disposal depends on the treatment technologies applied and spontaneously occurring microbiological processes. From different ages urban sewage sludge, 12 strains of bacteria with simultaneous...
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| Format: | Article |
| Language: | English |
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Springer
2024-01-01
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| Series: | Stress Biology |
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| Online Access: | https://doi.org/10.1007/s44154-023-00144-8 |
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| author | L. Perelomov V. D. Rajput M. Gertsen O. Sizova I. Perelomova S. Kozmenko T. Minkina Y. Atroshchenko |
| author_facet | L. Perelomov V. D. Rajput M. Gertsen O. Sizova I. Perelomova S. Kozmenko T. Minkina Y. Atroshchenko |
| author_sort | L. Perelomov |
| collection | DOAJ |
| description | Abstract Worldwide wastewater treatment plants generate enormous amounts of sewage sludge, and their further disposal depends on the treatment technologies applied and spontaneously occurring microbiological processes. From different ages urban sewage sludge, 12 strains of bacteria with simultaneous tolerance to two or more trace elements: Co, Ni, Cu, Zn, Cd and Pb at concentration of 3-5 mmol were isolated and identified by PCR of target genes and Sanger sequencing methods. The isloated metal(loids) tolerant strains belong to the species, i.e., Serratia fonticola, Rhodococcus qingshengii, Pseudomonas fragi, Pseudomonas extremaustralis, Pseudomonas cedrina, Stenotrophomonas maltophilia, Serratia liquefaciens and Citrobacter freundii. The ecological features of the isolated strains were studied. The optimal growth temperatures for most strains was 15–30°C at pH range of 5–9, although some strains grew at 7°C (Pseudomonas fragi SS0-4, Serratia fonticola SS0-9 and Serratia fonticola SS12-11). Satisfactory growth of two strains (Serratia fonticola SS0-1and Citrobacter freundii SS60-12) was noted in an acidic medium at pH 4. Most of the strains grew in the NaCl concentration range of 1–5%. The isolated bacteria resistant to high concentrations of trace elements can be used for the effective mineralization of sewage sludge and for the decontamination of wastewater. |
| first_indexed | 2024-03-08T10:01:23Z |
| format | Article |
| id | doaj.art-fee4201a3e6b4d5392048f3cdf275ce3 |
| institution | Directory Open Access Journal |
| issn | 2731-0450 |
| language | English |
| last_indexed | 2024-03-08T10:01:23Z |
| publishDate | 2024-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Stress Biology |
| spelling | doaj.art-fee4201a3e6b4d5392048f3cdf275ce32024-01-29T10:58:42ZengSpringerStress Biology2731-04502024-01-014111310.1007/s44154-023-00144-8Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plantL. Perelomov0V. D. Rajput1M. Gertsen2O. Sizova3I. Perelomova4S. Kozmenko5T. Minkina6Y. Atroshchenko7Tula State Lev Tolstoy Pedagogical University (Lev Tolstoy University)Academy of Biology and Biotechnology, Southern Federal UniversityTula State Lev Tolstoy Pedagogical University (Lev Tolstoy University)Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of RASTula State UniversityTula State Lev Tolstoy Pedagogical University (Lev Tolstoy University)Academy of Biology and Biotechnology, Southern Federal UniversityTula State Lev Tolstoy Pedagogical University (Lev Tolstoy University)Abstract Worldwide wastewater treatment plants generate enormous amounts of sewage sludge, and their further disposal depends on the treatment technologies applied and spontaneously occurring microbiological processes. From different ages urban sewage sludge, 12 strains of bacteria with simultaneous tolerance to two or more trace elements: Co, Ni, Cu, Zn, Cd and Pb at concentration of 3-5 mmol were isolated and identified by PCR of target genes and Sanger sequencing methods. The isloated metal(loids) tolerant strains belong to the species, i.e., Serratia fonticola, Rhodococcus qingshengii, Pseudomonas fragi, Pseudomonas extremaustralis, Pseudomonas cedrina, Stenotrophomonas maltophilia, Serratia liquefaciens and Citrobacter freundii. The ecological features of the isolated strains were studied. The optimal growth temperatures for most strains was 15–30°C at pH range of 5–9, although some strains grew at 7°C (Pseudomonas fragi SS0-4, Serratia fonticola SS0-9 and Serratia fonticola SS12-11). Satisfactory growth of two strains (Serratia fonticola SS0-1and Citrobacter freundii SS60-12) was noted in an acidic medium at pH 4. Most of the strains grew in the NaCl concentration range of 1–5%. The isolated bacteria resistant to high concentrations of trace elements can be used for the effective mineralization of sewage sludge and for the decontamination of wastewater.https://doi.org/10.1007/s44154-023-00144-8MicroorganismsHeavy metalsMetalloidsWastewater treatment plantSewage sludge |
| spellingShingle | L. Perelomov V. D. Rajput M. Gertsen O. Sizova I. Perelomova S. Kozmenko T. Minkina Y. Atroshchenko Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant Stress Biology Microorganisms Heavy metals Metalloids Wastewater treatment plant Sewage sludge |
| title | Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant |
| title_full | Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant |
| title_fullStr | Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant |
| title_full_unstemmed | Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant |
| title_short | Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant |
| title_sort | ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant |
| topic | Microorganisms Heavy metals Metalloids Wastewater treatment plant Sewage sludge |
| url | https://doi.org/10.1007/s44154-023-00144-8 |
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