Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes
Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive diseases such as streptococcal toxic shock syndrome, similar to that caused by S. pyogenes (GAS). Invasive SDSE infections are increasing, particularly among patients with diabetes mellitus. Here we investigate the associati...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02128/full |
| _version_ | 1818493362156601344 |
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| author | Kohei Ogura Kohei Ogura Kayo Okumura Yukiko Shimizu Teruo Kirikae Teruo Kirikae Tohru Miyoshi-Akiyama |
| author_facet | Kohei Ogura Kohei Ogura Kayo Okumura Yukiko Shimizu Teruo Kirikae Teruo Kirikae Tohru Miyoshi-Akiyama |
| author_sort | Kohei Ogura |
| collection | DOAJ |
| description | Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive diseases such as streptococcal toxic shock syndrome, similar to that caused by S. pyogenes (GAS). Invasive SDSE infections are increasing, particularly among patients with diabetes mellitus. Here we investigate the association between the pathogenicity of SDSE and diabetes mellitus in a mouse model, using GAS infection for comparison. Intraperitoneal injection of highly hemolytic SDSE-167 into C57BL6/J mice induced a rapid rise in blood glucose concentrations within 4 h, which was otherwise seen only in mice injected with high doses of hypervirulent GAS mutants. The survival rates of mice injected with SDSE-167 were significantly lower in mice (db/db) with type 2 diabetes than in nondiabetic mice. Injection of db/db mice with SDSE-167 increased the concentrations of cytokines and chemokines, particularly those of interleukin 6 and monocyte chemotactic protein-1. Microarray data indicate that multiple pathways are involved in the pathogenicity of SDSE-167 in db/db mice. These data reveal that the mechanisms underlying streptococcal infection differ between SDSE and GAS. |
| first_indexed | 2024-12-10T17:54:14Z |
| format | Article |
| id | doaj.art-fee62189c8c84e8ea21b49a81fc1f7f8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T17:54:14Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fee62189c8c84e8ea21b49a81fc1f7f82022-12-22T01:38:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02128403808Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of DiabetesKohei Ogura0Kohei Ogura1Kayo Okumura2Yukiko Shimizu3Teruo Kirikae4Teruo Kirikae5Tohru Miyoshi-Akiyama6Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo, JapanAdvanced Health Care Science Research Unit, Institute for Frontier Science Initiative, Ishikawa, JapanDepartment of Infectious Disease, Research Institute, National Center for Global Health and Medicine, Tokyo, JapanDepartment of Infectious Disease, Research Institute, National Center for Global Health and Medicine, Tokyo, JapanDepartment of Infectious Disease, Research Institute, National Center for Global Health and Medicine, Tokyo, JapanDepartment of Microbiology, Juntendo University School of Medicine, Tokyo, JapanPathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo, JapanStreptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive diseases such as streptococcal toxic shock syndrome, similar to that caused by S. pyogenes (GAS). Invasive SDSE infections are increasing, particularly among patients with diabetes mellitus. Here we investigate the association between the pathogenicity of SDSE and diabetes mellitus in a mouse model, using GAS infection for comparison. Intraperitoneal injection of highly hemolytic SDSE-167 into C57BL6/J mice induced a rapid rise in blood glucose concentrations within 4 h, which was otherwise seen only in mice injected with high doses of hypervirulent GAS mutants. The survival rates of mice injected with SDSE-167 were significantly lower in mice (db/db) with type 2 diabetes than in nondiabetic mice. Injection of db/db mice with SDSE-167 increased the concentrations of cytokines and chemokines, particularly those of interleukin 6 and monocyte chemotactic protein-1. Microarray data indicate that multiple pathways are involved in the pathogenicity of SDSE-167 in db/db mice. These data reveal that the mechanisms underlying streptococcal infection differ between SDSE and GAS.https://www.frontiersin.org/article/10.3389/fmicb.2018.02128/fullStreptococcus dysgalactiae subsp. equisimilisdiabetes mellitusdb/db mousecytokinelethalityinflammation |
| spellingShingle | Kohei Ogura Kohei Ogura Kayo Okumura Yukiko Shimizu Teruo Kirikae Teruo Kirikae Tohru Miyoshi-Akiyama Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes Frontiers in Microbiology Streptococcus dysgalactiae subsp. equisimilis diabetes mellitus db/db mouse cytokine lethality inflammation |
| title | Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes |
| title_full | Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes |
| title_fullStr | Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes |
| title_full_unstemmed | Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes |
| title_short | Pathogenicity Induced by Invasive Infection of Streptococcus dysgalactiae subsp. equisimilis in a Mouse Model of Diabetes |
| title_sort | pathogenicity induced by invasive infection of streptococcus dysgalactiae subsp equisimilis in a mouse model of diabetes |
| topic | Streptococcus dysgalactiae subsp. equisimilis diabetes mellitus db/db mouse cytokine lethality inflammation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02128/full |
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