Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels
Argania spinosa L. Skeels is an endemic species of west-central Morocco, which is characterized by a high diversity of morphological and genetic traits. It constitutes a natural resource for oleo-agro-sylvo-pastoral uses. All conservation and genetic breeding strategies aimed to domesticate argan r...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2023-03-01
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| Series: | Notulae Scientia Biologicae |
| Subjects: | |
| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11451 |
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| author | Ali EL BOUKHARI Salma TABI Abdelhamid EL MOUSADIK Rachida EL BOULLANI Abdelghani TAHIRI Meriyem KOUFAN Hamid BENYAHIA Rachid BOUHARROUD Naima AIT AABD |
| author_facet | Ali EL BOUKHARI Salma TABI Abdelhamid EL MOUSADIK Rachida EL BOULLANI Abdelghani TAHIRI Meriyem KOUFAN Hamid BENYAHIA Rachid BOUHARROUD Naima AIT AABD |
| author_sort | Ali EL BOUKHARI |
| collection | DOAJ |
| description |
Argania spinosa L. Skeels is an endemic species of west-central Morocco, which is characterized by a high diversity of morphological and genetic traits. It constitutes a natural resource for oleo-agro-sylvo-pastoral uses. All conservation and genetic breeding strategies aimed to domesticate argan require a good knowledge of the plant material. However, several studies focused on agronomical, morphological, phytochemical, and molecular characterization, while the cytogenetic aspects were less investigated. The objective of this work is to identify the chromosome number and ploidy level on the national argan collection at the Agadir Regional Agronomic Research Center, Morocco. The determination of the chromosome number was carried out on root tips of germinated seeds collected from five trees genotypes selected on various morphological aspects. As a result, chromosome count on active root tip cells showed variation in the number (2n = 20; 2n = 22; 2n = 24) with a stable ploidy level (2n = 2x) that is confirmed by flow cytometry. These results combine two previous findings (2n=20, 2n=24) and reveal a third existence of twenty-two chromosome. As a conclusion, A. spinosa has three chromosomal numbers which represent the genetic diversity of the chromosomal number that this species exhibits. More studies are required to explain this variation on chromosome numbers for future breeding programs and to avoid incompatibilities.
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| first_indexed | 2024-04-09T21:12:35Z |
| format | Article |
| id | doaj.art-fee6a09f41c0491f8d37d20994d81c56 |
| institution | Directory Open Access Journal |
| issn | 2067-3264 |
| language | English |
| last_indexed | 2024-04-09T21:12:35Z |
| publishDate | 2023-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-fee6a09f41c0491f8d37d20994d81c562023-03-28T17:04:31ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32642023-03-0115110.55779/nsb15111451Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) SkeelsAli EL BOUKHARI0Salma TABI1Abdelhamid EL MOUSADIK2Rachida EL BOULLANI3Abdelghani TAHIRI4Meriyem KOUFAN5Hamid BENYAHIA6Rachid BOUHARROUD7Naima AIT AABD8Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090; Ibn Zohr University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Natural Resources, PoB 8106, AgadirRegional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090; Sultan My Sliman University, Faculty of Sciences and Technics, Laboratory of Natural Resources, Environment and Health, B.P 523, Beni MellalIbn Zohr University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Natural Resources, PoB 8106, Agadir, Ibn Zohr University, Faculty of Sciences, Laboratory of Biotechnology and Valorization of Natural Resources, PoB 8106, Agadir, Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agronomic Research of Kenitra, National Institute of Agronomic Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090Regional Center of Agricultural Research of Agadir, National Institute of Agricultural Research (INRA), Avenue Ennasr, B.P. 415 Rabat Principale, Rabat 10090 Argania spinosa L. Skeels is an endemic species of west-central Morocco, which is characterized by a high diversity of morphological and genetic traits. It constitutes a natural resource for oleo-agro-sylvo-pastoral uses. All conservation and genetic breeding strategies aimed to domesticate argan require a good knowledge of the plant material. However, several studies focused on agronomical, morphological, phytochemical, and molecular characterization, while the cytogenetic aspects were less investigated. The objective of this work is to identify the chromosome number and ploidy level on the national argan collection at the Agadir Regional Agronomic Research Center, Morocco. The determination of the chromosome number was carried out on root tips of germinated seeds collected from five trees genotypes selected on various morphological aspects. As a result, chromosome count on active root tip cells showed variation in the number (2n = 20; 2n = 22; 2n = 24) with a stable ploidy level (2n = 2x) that is confirmed by flow cytometry. These results combine two previous findings (2n=20, 2n=24) and reveal a third existence of twenty-two chromosome. As a conclusion, A. spinosa has three chromosomal numbers which represent the genetic diversity of the chromosomal number that this species exhibits. More studies are required to explain this variation on chromosome numbers for future breeding programs and to avoid incompatibilities. https://www.notulaebiologicae.ro/index.php/nsb/article/view/11451Argane treechromosome numbercytogeneticflow cytometrygenetic diversity |
| spellingShingle | Ali EL BOUKHARI Salma TABI Abdelhamid EL MOUSADIK Rachida EL BOULLANI Abdelghani TAHIRI Meriyem KOUFAN Hamid BENYAHIA Rachid BOUHARROUD Naima AIT AABD Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels Notulae Scientia Biologicae Argane tree chromosome number cytogenetic flow cytometry genetic diversity |
| title | Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels |
| title_full | Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels |
| title_fullStr | Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels |
| title_full_unstemmed | Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels |
| title_short | Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels |
| title_sort | flow cytometry and chromosome numbers variation in argan tree argania spinosa l skeels |
| topic | Argane tree chromosome number cytogenetic flow cytometry genetic diversity |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/11451 |
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