Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine
Abstract The second generation of H1 antihistamines from the piperidine group are often used for treating allergic diseases due to their action on histaminic receptors, the primary mediator of allergy. Moreover, the antihistamines have anti-inflammatory action, mediated through platelet-activating f...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-01-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100889&tlng=en |
| _version_ | 1797951350101770240 |
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| author | Cintia Maria Alves Mothé Aline de Souza Anil Kumar Singh Nádia Araci Bou-Chacra María Segunda Aurora-Prado |
| author_facet | Cintia Maria Alves Mothé Aline de Souza Anil Kumar Singh Nádia Araci Bou-Chacra María Segunda Aurora-Prado |
| author_sort | Cintia Maria Alves Mothé |
| collection | DOAJ |
| description | Abstract The second generation of H1 antihistamines from the piperidine group are often used for treating allergic diseases due to their action on histaminic receptors, the primary mediator of allergy. Moreover, the antihistamines have anti-inflammatory action, mediated through platelet-activating factor blocking activity. A simple and rapid capillary zone electrophoresis method was developed and validated for the determination of loratadine (LOR) and rupatadine (RUP) in tablets. The analyses were carried out using a fused silica capillary of 50.2 cm (40 cm effective length), 75 µm i.d. The background electrolyte was composed of boric acid 35 mmol/L, pH 2.5. Voltage of 20 kV, hydrodynamic injection of 3447.3 Pa for 3s, temperature at 25 ºC, and UV detection at 205 nm were applied. Electrophoretic separation was achieved at 1.8 and 2.8 min for RUP and LOR, respectively. The method was linear for both drugs in a range of 50.0 to 400.0 μg/mL (r>0.99). The limits of detection and quantification were 46.37 and 140.52 μg/mL, for LOR and 29.60 and 89.69 μg/mL for RUP respectively. The precision was less than 5.0 % for both drugs. The average recovery was approximately 100 %. The proposed novel method can significantly contribute to the rapid detection of counterfeit products and in quality control of drug products containing antihistamines. |
| first_indexed | 2024-04-10T22:30:19Z |
| format | Article |
| id | doaj.art-fee7a685d3a945d8b2b0f210310a2d55 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-10T22:30:19Z |
| publishDate | 2023-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-fee7a685d3a945d8b2b0f210310a2d552023-01-17T07:34:04ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-01-015810.1590/s2175-97902022e20767Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadineCintia Maria Alves Mothéhttps://orcid.org/0000-0002-4683-2676Aline de Souzahttps://orcid.org/0000-0003-4307-6497Anil Kumar SinghNádia Araci Bou-Chacrahttps://orcid.org/0000-0002-5271-5468María Segunda Aurora-Pradohttps://orcid.org/0000-0002-9956-9410Abstract The second generation of H1 antihistamines from the piperidine group are often used for treating allergic diseases due to their action on histaminic receptors, the primary mediator of allergy. Moreover, the antihistamines have anti-inflammatory action, mediated through platelet-activating factor blocking activity. A simple and rapid capillary zone electrophoresis method was developed and validated for the determination of loratadine (LOR) and rupatadine (RUP) in tablets. The analyses were carried out using a fused silica capillary of 50.2 cm (40 cm effective length), 75 µm i.d. The background electrolyte was composed of boric acid 35 mmol/L, pH 2.5. Voltage of 20 kV, hydrodynamic injection of 3447.3 Pa for 3s, temperature at 25 ºC, and UV detection at 205 nm were applied. Electrophoretic separation was achieved at 1.8 and 2.8 min for RUP and LOR, respectively. The method was linear for both drugs in a range of 50.0 to 400.0 μg/mL (r>0.99). The limits of detection and quantification were 46.37 and 140.52 μg/mL, for LOR and 29.60 and 89.69 μg/mL for RUP respectively. The precision was less than 5.0 % for both drugs. The average recovery was approximately 100 %. The proposed novel method can significantly contribute to the rapid detection of counterfeit products and in quality control of drug products containing antihistamines.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100889&tlng=enAntihistaminesQuality controlCapillary electrophoresisAnalytical method development |
| spellingShingle | Cintia Maria Alves Mothé Aline de Souza Anil Kumar Singh Nádia Araci Bou-Chacra María Segunda Aurora-Prado Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine Brazilian Journal of Pharmaceutical Sciences Antihistamines Quality control Capillary electrophoresis Analytical method development |
| title | Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine |
| title_full | Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine |
| title_fullStr | Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine |
| title_full_unstemmed | Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine |
| title_short | Application of capillary zone electrophoresis to determine second-generation H1 antihistaminic drugs, loratadine and rupatadine |
| title_sort | application of capillary zone electrophoresis to determine second generation h1 antihistaminic drugs loratadine and rupatadine |
| topic | Antihistamines Quality control Capillary electrophoresis Analytical method development |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100889&tlng=en |
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