Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia)
The objective: to analyze the association of the Asp299Gln (rs 4986790) and Thr399Ile(rs 4986791) polymorphisms of the TLR4 gene with the risk of pulmonary sarcoidosis in the Russian population of the Republic of Karelia.Subjects and Methods. 253 people were examined: 122 patients diagnosed with mor...
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2022-10-01
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| Series: | Туберкулез и болезни лёгких |
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| Online Access: | https://www.tibl-journal.com/jour/article/view/1672 |
| _version_ | 1826563026125324288 |
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| author | I. E. Malysheva L. V. Topchieva E. L. Tikhonovich |
| author_facet | I. E. Malysheva L. V. Topchieva E. L. Tikhonovich |
| author_sort | I. E. Malysheva |
| collection | DOAJ |
| description | The objective: to analyze the association of the Asp299Gln (rs 4986790) and Thr399Ile(rs 4986791) polymorphisms of the TLR4 gene with the risk of pulmonary sarcoidosis in the Russian population of the Republic of Karelia.Subjects and Methods. 253 people were examined: 122 patients diagnosed with morphologically verified sarcoidosis with lung involvement (average age – 41.00 ± 12.56 years) (Sarcoidosis Group) and 131 healthy donors (Control Group) (average age – 44.00 ± 14.23 years). All subjects in both groups considered themselves Russians by ethnicity, as reported in the survey. The distribution of alleles and genotypes by the polymorphic markers Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) of the TLR4 gene was studied in the group of patients with pulmonary sarcoidosis and the group of healthy individuals. Genotyping was carried out by PCR-RFLP.Results. There were no statistically significant differences in the distribution of allele and genotype frequencies for the Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) polymorphic markers of the TLR4 gene between the group of patients with pulmonary sarcoidosis and the control group: (χ2 = 0, df = 1, p = 0.997 и χ2 = 1.0, df = 2, p = 0.606; χ2 = 0.785, df = 1, p = 0.376 и χ2 = 0, df = 2, p = 1.0).Conclusion. The polymorphic marker Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) of the TLR4 gene is not associated with the risk of pulmonary sarcoidosis in the Russian population of the Republic of Karelia. |
| first_indexed | 2024-03-12T04:24:31Z |
| format | Article |
| id | doaj.art-fee7e12e81ef46ec9f8c0a116edced6a |
| institution | Directory Open Access Journal |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| last_indexed | 2025-03-14T09:57:25Z |
| publishDate | 2022-10-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj.art-fee7e12e81ef46ec9f8c0a116edced6a2025-03-02T11:22:35ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062022-10-011009162010.21292/2075-1230-2022-100-9-16-201667Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia)I. E. Malysheva0L. V. Topchieva1E. L. Tikhonovich2Institute of Biology of Karelian Research Center of the Russian Academy of SciencesInstitute of Biology of Karelian Research Center of the Russian Academy of SciencesV. A. Baranov Republican HospitalThe objective: to analyze the association of the Asp299Gln (rs 4986790) and Thr399Ile(rs 4986791) polymorphisms of the TLR4 gene with the risk of pulmonary sarcoidosis in the Russian population of the Republic of Karelia.Subjects and Methods. 253 people were examined: 122 patients diagnosed with morphologically verified sarcoidosis with lung involvement (average age – 41.00 ± 12.56 years) (Sarcoidosis Group) and 131 healthy donors (Control Group) (average age – 44.00 ± 14.23 years). All subjects in both groups considered themselves Russians by ethnicity, as reported in the survey. The distribution of alleles and genotypes by the polymorphic markers Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) of the TLR4 gene was studied in the group of patients with pulmonary sarcoidosis and the group of healthy individuals. Genotyping was carried out by PCR-RFLP.Results. There were no statistically significant differences in the distribution of allele and genotype frequencies for the Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) polymorphic markers of the TLR4 gene between the group of patients with pulmonary sarcoidosis and the control group: (χ2 = 0, df = 1, p = 0.997 и χ2 = 1.0, df = 2, p = 0.606; χ2 = 0.785, df = 1, p = 0.376 и χ2 = 0, df = 2, p = 1.0).Conclusion. The polymorphic marker Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) of the TLR4 gene is not associated with the risk of pulmonary sarcoidosis in the Russian population of the Republic of Karelia.https://www.tibl-journal.com/jour/article/view/1672pulmonary sarcoidosistoll-like receptors (tlrs)tlr4genepolymorphism |
| spellingShingle | I. E. Malysheva L. V. Topchieva E. L. Tikhonovich Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia) Туберкулез и болезни лёгких pulmonary sarcoidosis toll-like receptors (tlrs) tlr4gene polymorphism |
| title | Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia) |
| title_full | Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia) |
| title_fullStr | Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia) |
| title_full_unstemmed | Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia) |
| title_short | Analysis of Association of Asp299Gln (rs 4986790) and Thr399Ile (rs 4986791) Polymorphisms of the <i>TLR4</i> Gene with Risk of Developing Lung Sarcoidosis (Using the Example of Residents of Karelia) |
| title_sort | analysis of association of asp299gln rs 4986790 and thr399ile rs 4986791 polymorphisms of the i tlr4 i gene with risk of developing lung sarcoidosis using the example of residents of karelia |
| topic | pulmonary sarcoidosis toll-like receptors (tlrs) tlr4gene polymorphism |
| url | https://www.tibl-journal.com/jour/article/view/1672 |
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