Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone
Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (<i>Hdh-CAT</i>) in stress physiology, innate immunity, testicular development,...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/109 |
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| author | Shaharior Hossen Zahid Parvez Sukhan Soo Cheol Kim Md. Abu Hanif Il-Keun Kong Kang Hee Kho |
| author_facet | Shaharior Hossen Zahid Parvez Sukhan Soo Cheol Kim Md. Abu Hanif Il-Keun Kong Kang Hee Kho |
| author_sort | Shaharior Hossen |
| collection | DOAJ |
| description | Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (<i>Hdh-CAT</i>) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. <i>Hdh-CAT</i> gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of <i>Hdh-CAT</i> had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence <i>in situ</i> hybridization confirmed <i>Hdh-CAT</i> localization in the digestive tubules of the DG. <i>Hdh-CAT</i> was induced by different types of stress including thermal stress, H<sub>2</sub>O<sub>2</sub> induction, and starvation. Immune challenges with <i>Vibrio</i>, lipopolysaccharides, and polyinosinic–polycytidylic acid sodium salt also upregulated <i>Hdh-CAT</i> mRNA expression and catalase activity. <i>Hdh-CAT</i> responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered <i>Hdh-CAT</i> mRNA expression. <i>Hdh-CAT</i> mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower <i>Hdh-CAT</i> mRNA expression levels compared with fresh sperm. <i>Hdh-CAT</i> mRNA expression showed a relationship with the production of ROS. These results suggest that <i>Hdh-CAT</i> might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone. |
| first_indexed | 2024-03-09T13:47:44Z |
| format | Article |
| id | doaj.art-feea8e395fd44b5c9d388963c904845c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:47:44Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-feea8e395fd44b5c9d388963c904845c2023-11-30T20:58:26ZengMDPI AGAntioxidants2076-39212023-01-0112110910.3390/antiox12010109Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific AbaloneShaharior Hossen0Zahid Parvez Sukhan1Soo Cheol Kim2Md. Abu Hanif3Il-Keun Kong4Kang Hee Kho5Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50 Daehak-ro, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50 Daehak-ro, Yeosu 59626, Republic of KoreaSouth Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Republic of KoreaDepartment of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50 Daehak-ro, Yeosu 59626, Republic of KoreaDepartment of Animal Science, Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, 50 Daehak-ro, Yeosu 59626, Republic of KoreaCatalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (<i>Hdh-CAT</i>) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. <i>Hdh-CAT</i> gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of <i>Hdh-CAT</i> had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence <i>in situ</i> hybridization confirmed <i>Hdh-CAT</i> localization in the digestive tubules of the DG. <i>Hdh-CAT</i> was induced by different types of stress including thermal stress, H<sub>2</sub>O<sub>2</sub> induction, and starvation. Immune challenges with <i>Vibrio</i>, lipopolysaccharides, and polyinosinic–polycytidylic acid sodium salt also upregulated <i>Hdh-CAT</i> mRNA expression and catalase activity. <i>Hdh-CAT</i> responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered <i>Hdh-CAT</i> mRNA expression. <i>Hdh-CAT</i> mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower <i>Hdh-CAT</i> mRNA expression levels compared with fresh sperm. <i>Hdh-CAT</i> mRNA expression showed a relationship with the production of ROS. These results suggest that <i>Hdh-CAT</i> might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone.https://www.mdpi.com/2076-3921/12/1/109catalaseinnate immunitystress physiologystarvationmetamorphosiscryopreserved sperm |
| spellingShingle | Shaharior Hossen Zahid Parvez Sukhan Soo Cheol Kim Md. Abu Hanif Il-Keun Kong Kang Hee Kho Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone Antioxidants catalase innate immunity stress physiology starvation metamorphosis cryopreserved sperm |
| title | Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone |
| title_full | Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone |
| title_fullStr | Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone |
| title_full_unstemmed | Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone |
| title_short | Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone |
| title_sort | molecular cloning and functional characterization of catalase in stress physiology innate immunity testicular development metamorphosis and cryopreserved sperm of pacific abalone |
| topic | catalase innate immunity stress physiology starvation metamorphosis cryopreserved sperm |
| url | https://www.mdpi.com/2076-3921/12/1/109 |
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