Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable...

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Main Authors: Çiğdem Alev Özel, Fatma Ünal
Format: Article
Language:English
Published: Turkish Science and Technology Publishing (TURSTEP) 2016-12-01
Series:Turkish Journal of Agriculture: Food Science and Technology
Subjects:
Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/829
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author Çiğdem Alev Özel
Fatma Ünal
author_facet Çiğdem Alev Özel
Fatma Ünal
author_sort Çiğdem Alev Özel
collection DOAJ
description Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.
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spelling doaj.art-feebdc650870453c846673db5aca5b0c2023-02-15T16:08:44ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2016-12-014121173117810.24925/turjaf.v4i12.1173-1178.829442Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic AcidÇiğdem Alev Özel0Fatma Ünal1Department of Biology Education, Faculty of Gazi Education, Gazi University, Teknikokullar, 06500 AnkaraDepartment of Biology, Faculty of Science, Gazi University, Teknikokullar, 06500 AnkaraMuscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.http://www.agrifoodscience.com/index.php/TURJAF/article/view/829AcclimatisationmicropropagationBulbous PlantRegenerationRooting
spellingShingle Çiğdem Alev Özel
Fatma Ünal
Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
Turkish Journal of Agriculture: Food Science and Technology
Acclimatisation
micropropagation
Bulbous Plant
Regeneration
Rooting
title Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
title_full Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
title_fullStr Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
title_full_unstemmed Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
title_short Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
title_sort efficient in vitro clonal propagation of muscari neglectum guss ex ten using thidiazuron α naphthalene acetic acid
topic Acclimatisation
micropropagation
Bulbous Plant
Regeneration
Rooting
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/829
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