Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid
Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable...
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Format: | Article |
Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2016-12-01
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Series: | Turkish Journal of Agriculture: Food Science and Technology |
Subjects: | |
Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/829 |
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author | Çiğdem Alev Özel Fatma Ünal |
author_facet | Çiğdem Alev Özel Fatma Ünal |
author_sort | Çiğdem Alev Özel |
collection | DOAJ |
description | Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant. |
first_indexed | 2024-04-10T14:32:19Z |
format | Article |
id | doaj.art-feebdc650870453c846673db5aca5b0c |
institution | Directory Open Access Journal |
issn | 2148-127X |
language | English |
last_indexed | 2024-04-10T14:32:19Z |
publishDate | 2016-12-01 |
publisher | Turkish Science and Technology Publishing (TURSTEP) |
record_format | Article |
series | Turkish Journal of Agriculture: Food Science and Technology |
spelling | doaj.art-feebdc650870453c846673db5aca5b0c2023-02-15T16:08:44ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2016-12-014121173117810.24925/turjaf.v4i12.1173-1178.829442Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic AcidÇiğdem Alev Özel0Fatma Ünal1Department of Biology Education, Faculty of Gazi Education, Gazi University, Teknikokullar, 06500 AnkaraDepartment of Biology, Faculty of Science, Gazi University, Teknikokullar, 06500 AnkaraMuscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.http://www.agrifoodscience.com/index.php/TURJAF/article/view/829AcclimatisationmicropropagationBulbous PlantRegenerationRooting |
spellingShingle | Çiğdem Alev Özel Fatma Ünal Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid Turkish Journal of Agriculture: Food Science and Technology Acclimatisation micropropagation Bulbous Plant Regeneration Rooting |
title | Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid |
title_full | Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid |
title_fullStr | Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid |
title_full_unstemmed | Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid |
title_short | Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid |
title_sort | efficient in vitro clonal propagation of muscari neglectum guss ex ten using thidiazuron α naphthalene acetic acid |
topic | Acclimatisation micropropagation Bulbous Plant Regeneration Rooting |
url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/829 |
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