Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity
We evaluated the effect of endobacteria <i>Bacillus subtilis</i> (strain 10–4) as a co-inoculant for promoting plant growth and redox metabolism in two contrasting genotypes of <i>Triticum aestivum</i> L. (wheat): Ekada70 (drought tolerant (DT)) and Salavat Yulaev (drought su...
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MDPI AG
2021-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/12/2557 |
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| author | Dilara Maslennikova Oksana Lastochkina |
| author_facet | Dilara Maslennikova Oksana Lastochkina |
| author_sort | Dilara Maslennikova |
| collection | DOAJ |
| description | We evaluated the effect of endobacteria <i>Bacillus subtilis</i> (strain 10–4) as a co-inoculant for promoting plant growth and redox metabolism in two contrasting genotypes of <i>Triticum aestivum</i> L. (wheat): Ekada70 (drought tolerant (DT)) and Salavat Yulaev (drought susceptible (DS)) in early stages of adaptation to drought (12% PEG–6000). Results revealed that drought reduced growth and dramatically augmented oxidative stress markers, i.e., hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipid peroxidation (MDA). Furthermore, the depletion of ascorbate (AsA) and glutathione (GSH), accompanied by a significant activation of ascorbate peroxidase (APX) and glutathione reductase (GR), in both stressed wheat cultivars (which was more pronounced in DS genotype) was found. <i>B. subtilis</i> had a protective effect on growth and antioxidant status, wherein the stabilization of AsA and GSH levels was revealed. This was accompanied by a decrease of drought-caused APX and GR activation in DS plants, while in DT plants additional antioxidant accumulation and GR activation were observed. H<sub>2</sub>O<sub>2</sub> and MDA were considerably reduced in both drought-stressed wheat genotypes because of the application of <i>B. subtilis</i>. Thus, the findings suggest the key roles in <i>B. subtilis</i>-mediated drought tolerance in DS cv. Salavat Yulaev and DT cv. Ekada70 played are AsA and GSH, respectively; which, in both cases, resulted in reduced cell oxidative damage and improved growth in seedlings under drought. |
| first_indexed | 2024-03-10T03:18:03Z |
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| language | English |
| last_indexed | 2024-03-10T03:18:03Z |
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| series | Plants |
| spelling | doaj.art-feec65345e104c8d83b483e00dc72eed2023-11-23T10:09:54ZengMDPI AGPlants2223-77472021-11-011012255710.3390/plants10122557Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought SensitivityDilara Maslennikova0Oksana Lastochkina1Institute of Biochemistry and Genetics, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaInstitute of Biochemistry and Genetics, Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, RussiaWe evaluated the effect of endobacteria <i>Bacillus subtilis</i> (strain 10–4) as a co-inoculant for promoting plant growth and redox metabolism in two contrasting genotypes of <i>Triticum aestivum</i> L. (wheat): Ekada70 (drought tolerant (DT)) and Salavat Yulaev (drought susceptible (DS)) in early stages of adaptation to drought (12% PEG–6000). Results revealed that drought reduced growth and dramatically augmented oxidative stress markers, i.e., hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and lipid peroxidation (MDA). Furthermore, the depletion of ascorbate (AsA) and glutathione (GSH), accompanied by a significant activation of ascorbate peroxidase (APX) and glutathione reductase (GR), in both stressed wheat cultivars (which was more pronounced in DS genotype) was found. <i>B. subtilis</i> had a protective effect on growth and antioxidant status, wherein the stabilization of AsA and GSH levels was revealed. This was accompanied by a decrease of drought-caused APX and GR activation in DS plants, while in DT plants additional antioxidant accumulation and GR activation were observed. H<sub>2</sub>O<sub>2</sub> and MDA were considerably reduced in both drought-stressed wheat genotypes because of the application of <i>B. subtilis</i>. Thus, the findings suggest the key roles in <i>B. subtilis</i>-mediated drought tolerance in DS cv. Salavat Yulaev and DT cv. Ekada70 played are AsA and GSH, respectively; which, in both cases, resulted in reduced cell oxidative damage and improved growth in seedlings under drought.https://www.mdpi.com/2223-7747/10/12/2557endophytic bacteria<i>Bacillus subtilis</i><i>Triticum aestivum</i> L.droughttolerancehydrogen peroxide |
| spellingShingle | Dilara Maslennikova Oksana Lastochkina Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity Plants endophytic bacteria <i>Bacillus subtilis</i> <i>Triticum aestivum</i> L. drought tolerance hydrogen peroxide |
| title | Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity |
| title_full | Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity |
| title_fullStr | Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity |
| title_full_unstemmed | Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity |
| title_short | Contribution of Ascorbate and Glutathione in Endobacteria <i>Bacillus subtilis</i>-Mediated Drought Tolerance in Two <i>Triticum aestivum</i> L. Genotypes Contrasting in Drought Sensitivity |
| title_sort | contribution of ascorbate and glutathione in endobacteria i bacillus subtilis i mediated drought tolerance in two i triticum aestivum i l genotypes contrasting in drought sensitivity |
| topic | endophytic bacteria <i>Bacillus subtilis</i> <i>Triticum aestivum</i> L. drought tolerance hydrogen peroxide |
| url | https://www.mdpi.com/2223-7747/10/12/2557 |
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