Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i>
<i>Salvia miltiorrhiza</i> is an important medicinal plant, which is mainly used for treatment of cardiovascular and cerebrovascular diseases. GATA transcription factors are evolutionarily conser-ved proteins that play essential roles in biological process of plants. In this study, we sy...
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MDPI AG
2022-05-01
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| author | Haiyan Li Tianrang Liu Biao Wang Hongbo Li |
| author_facet | Haiyan Li Tianrang Liu Biao Wang Hongbo Li |
| author_sort | Haiyan Li |
| collection | DOAJ |
| description | <i>Salvia miltiorrhiza</i> is an important medicinal plant, which is mainly used for treatment of cardiovascular and cerebrovascular diseases. GATA transcription factors are evolutionarily conser-ved proteins that play essential roles in biological process of plants. In this study, we systematically characterized the GATA transcription factors in <i>S. miltiorrhiza</i>. A total 28 <i>SmGATA</i> genes were identified and divided into four subfamilies based on phylogenetic analysis and domain. <i>SmGATA</i> genes being clustered into a subfamily have similar conserved motifs and exon-intron patterns, and unevenly distribute on eight chromosomes of <i>S. miltiorrhiza</i>. Tissue-specific expression analysis based on transcriptome datasets showed that the majority of <i>SmGATA</i> genes were preferentially expressed in roots. Under methyl jasmonate (MeJA) treatment, the quantitative real-time PCR (qRT-PCR) analysis indicated that several <i>SmGATA</i> genes in roots showed distinct upregulation post-MeJA treatment, especially <i>SmGATA08</i>, which was highly responsive to MeJA, and might be involved in the jasmonate signal, thereby affecting root growth, development, tolerance to various stresses, or secondary metabolites biosynthesis. The study found that several <i>SmGATA</i>s, like <i>SmGATA08</i>, are highly responsive to MeJA, indicating that these <i>SmGATA</i>s might be vital in the biosynthesis of tanshinones and phenolic acids by regulating the response to MeJA in <i>S. miltiorrhiza</i>. Our results laid the foundation for understanding their biological roles and quality improvement in <i>S. miltiorrhiza</i>. |
| first_indexed | 2024-03-10T03:50:50Z |
| format | Article |
| id | doaj.art-feed1b18e3484b2a88ff450b930c5820 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T03:50:50Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-feed1b18e3484b2a88ff450b930c58202023-11-23T11:10:00ZengMDPI AGGenes2073-44252022-05-0113582210.3390/genes13050822Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i>Haiyan Li0Tianrang Liu1Biao Wang2Hongbo Li3College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, China<i>Salvia miltiorrhiza</i> is an important medicinal plant, which is mainly used for treatment of cardiovascular and cerebrovascular diseases. GATA transcription factors are evolutionarily conser-ved proteins that play essential roles in biological process of plants. In this study, we systematically characterized the GATA transcription factors in <i>S. miltiorrhiza</i>. A total 28 <i>SmGATA</i> genes were identified and divided into four subfamilies based on phylogenetic analysis and domain. <i>SmGATA</i> genes being clustered into a subfamily have similar conserved motifs and exon-intron patterns, and unevenly distribute on eight chromosomes of <i>S. miltiorrhiza</i>. Tissue-specific expression analysis based on transcriptome datasets showed that the majority of <i>SmGATA</i> genes were preferentially expressed in roots. Under methyl jasmonate (MeJA) treatment, the quantitative real-time PCR (qRT-PCR) analysis indicated that several <i>SmGATA</i> genes in roots showed distinct upregulation post-MeJA treatment, especially <i>SmGATA08</i>, which was highly responsive to MeJA, and might be involved in the jasmonate signal, thereby affecting root growth, development, tolerance to various stresses, or secondary metabolites biosynthesis. The study found that several <i>SmGATA</i>s, like <i>SmGATA08</i>, are highly responsive to MeJA, indicating that these <i>SmGATA</i>s might be vital in the biosynthesis of tanshinones and phenolic acids by regulating the response to MeJA in <i>S. miltiorrhiza</i>. Our results laid the foundation for understanding their biological roles and quality improvement in <i>S. miltiorrhiza</i>.https://www.mdpi.com/2073-4425/13/5/822<i>Salvia miltiorrhiza</i>GATA gene familygenome-wide characterizationMeJAexpression patterns |
| spellingShingle | Haiyan Li Tianrang Liu Biao Wang Hongbo Li Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i> Genes <i>Salvia miltiorrhiza</i> GATA gene family genome-wide characterization MeJA expression patterns |
| title | Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i> |
| title_full | Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i> |
| title_fullStr | Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i> |
| title_full_unstemmed | Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i> |
| title_short | Genome-Wide Characterization and Expression Analysis of GATA Transcription Factors in Response to Methyl Jasmonate in <i>Salvia miltiorrhiza</i> |
| title_sort | genome wide characterization and expression analysis of gata transcription factors in response to methyl jasmonate in i salvia miltiorrhiza i |
| topic | <i>Salvia miltiorrhiza</i> GATA gene family genome-wide characterization MeJA expression patterns |
| url | https://www.mdpi.com/2073-4425/13/5/822 |
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