Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects
As a tissue regeneration strategy, the utilization of mesenchymal stem cells (MSCs) has drawn considerable attention. Comprehensive research using MSCs has led to significant preclinical or clinical outcomes; however, improving the survival rate, engraftment efficacy, and immunogenicity of implanted...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/5/1078 |
| _version_ | 1797395273560883200 |
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| author | Han Young Kim Suk Ho Bhang |
| author_facet | Han Young Kim Suk Ho Bhang |
| author_sort | Han Young Kim |
| collection | DOAJ |
| description | As a tissue regeneration strategy, the utilization of mesenchymal stem cells (MSCs) has drawn considerable attention. Comprehensive research using MSCs has led to significant preclinical or clinical outcomes; however, improving the survival rate, engraftment efficacy, and immunogenicity of implanted MSCs remains challenging. Although MSC-derived exosomes were recently introduced and reported to have great potential to replace conventional MSC-based therapeutics, the poor production yield and heterogeneity of exosomes are critical hurdles for their further applications. Herein, we report the fabrication of exosome-mimetic MSC-engineered nanovesicles (MSC-NVs) by subjecting cells to serial extrusion through filters. The fabricated MSC-NVs exhibit a hydrodynamic size of ~120 nm, which is considerably smaller than the size of MSCs (~30 μm). MSC-NVs contain both MSC markers and exosome markers. Importantly, various therapeutic growth factors originating from parent MSCs are encapsulated in the MSC-NVs. The MSC-NVs exerted various therapeutic effects comparable to those of MSCs. They also significantly induced the angiogenesis of endothelial cells and showed neuroprotective effects in damaged neuronal cells. The results collectively demonstrate that the fabricated MSC-NVs can serve as a nanosized therapeutic agent for tissue regeneration. |
| first_indexed | 2024-03-09T00:32:02Z |
| format | Article |
| id | doaj.art-fef0571312634aaab69f9209f964c1f4 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T00:32:02Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-fef0571312634aaab69f9209f964c1f42023-12-11T18:29:04ZengMDPI AGMaterials1996-19442021-02-01145107810.3390/ma14051078Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective EffectsHan Young Kim0Suk Ho Bhang1Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, KoreaSchool of Chemical Engineering, Sungkyunkwan University, Suwon 16419, KoreaAs a tissue regeneration strategy, the utilization of mesenchymal stem cells (MSCs) has drawn considerable attention. Comprehensive research using MSCs has led to significant preclinical or clinical outcomes; however, improving the survival rate, engraftment efficacy, and immunogenicity of implanted MSCs remains challenging. Although MSC-derived exosomes were recently introduced and reported to have great potential to replace conventional MSC-based therapeutics, the poor production yield and heterogeneity of exosomes are critical hurdles for their further applications. Herein, we report the fabrication of exosome-mimetic MSC-engineered nanovesicles (MSC-NVs) by subjecting cells to serial extrusion through filters. The fabricated MSC-NVs exhibit a hydrodynamic size of ~120 nm, which is considerably smaller than the size of MSCs (~30 μm). MSC-NVs contain both MSC markers and exosome markers. Importantly, various therapeutic growth factors originating from parent MSCs are encapsulated in the MSC-NVs. The MSC-NVs exerted various therapeutic effects comparable to those of MSCs. They also significantly induced the angiogenesis of endothelial cells and showed neuroprotective effects in damaged neuronal cells. The results collectively demonstrate that the fabricated MSC-NVs can serve as a nanosized therapeutic agent for tissue regeneration.https://www.mdpi.com/1996-1944/14/5/1078angiogenesismesenchymal stem cellsnanovesiclesneuroprotective effect |
| spellingShingle | Han Young Kim Suk Ho Bhang Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects Materials angiogenesis mesenchymal stem cells nanovesicles neuroprotective effect |
| title | Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects |
| title_full | Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects |
| title_fullStr | Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects |
| title_full_unstemmed | Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects |
| title_short | Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects |
| title_sort | stem cell engineered nanovesicles exert proangiogenic and neuroprotective effects |
| topic | angiogenesis mesenchymal stem cells nanovesicles neuroprotective effect |
| url | https://www.mdpi.com/1996-1944/14/5/1078 |
| work_keys_str_mv | AT hanyoungkim stemcellengineerednanovesiclesexertproangiogenicandneuroprotectiveeffects AT sukhobhang stemcellengineerednanovesiclesexertproangiogenicandneuroprotectiveeffects |