Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue
Abstract Obesity and type 2 diabetes are two closely related diseases representing a serious threat worldwide. An increase in metabolic rate through enhancement of non-shivering thermogenesis in adipose tissue may represent a potential therapeutic strategy. Nevertheless, a better understanding of th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33367-w |
| _version_ | 1797832197616435200 |
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| author | Jordi Rodó Miquel Garcia Estefania Casana Sergio Muñoz Claudia Jambrina Victor Sacristan Sylvie Franckhauser Ignasi Grass Veronica Jimenez Fatima Bosch |
| author_facet | Jordi Rodó Miquel Garcia Estefania Casana Sergio Muñoz Claudia Jambrina Victor Sacristan Sylvie Franckhauser Ignasi Grass Veronica Jimenez Fatima Bosch |
| author_sort | Jordi Rodó |
| collection | DOAJ |
| description | Abstract Obesity and type 2 diabetes are two closely related diseases representing a serious threat worldwide. An increase in metabolic rate through enhancement of non-shivering thermogenesis in adipose tissue may represent a potential therapeutic strategy. Nevertheless, a better understanding of thermogenesis transcriptional regulation is needed to allow the development of new effective treatments. Here, we aimed to characterize the specific transcriptomic response of white and brown adipose tissues after thermogenic induction. Using cold exposure to induce thermogenesis in mice, we identified mRNAs and miRNAs that were differentially expressed in several adipose depots. In addition, integration of transcriptomic data in regulatory networks of miRNAs and transcription factors allowed the identification of key nodes likely controlling metabolism and immune response. Moreover, we identified the putative role of the transcription factor PU.1 in the regulation of PPARγ-mediated thermogenic response of subcutaneous white adipose tissue. Therefore, the present study provides new insights into the molecular mechanisms that regulate non-shivering thermogenesis. |
| first_indexed | 2024-04-09T14:03:56Z |
| format | Article |
| id | doaj.art-fef37098d6ab46d092022796ee2c6d0a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T14:03:56Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fef37098d6ab46d092022796ee2c6d0a2023-05-07T11:10:48ZengNature PortfolioScientific Reports2045-23222023-05-0113111310.1038/s41598-023-33367-wIntegrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissueJordi Rodó0Miquel Garcia1Estefania Casana2Sergio Muñoz3Claudia Jambrina4Victor Sacristan5Sylvie Franckhauser6Ignasi Grass7Veronica Jimenez8Fatima Bosch9Center of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaCenter of Animal Biotechnology and Gene Therapy, Universitat Autònoma de BarcelonaAbstract Obesity and type 2 diabetes are two closely related diseases representing a serious threat worldwide. An increase in metabolic rate through enhancement of non-shivering thermogenesis in adipose tissue may represent a potential therapeutic strategy. Nevertheless, a better understanding of thermogenesis transcriptional regulation is needed to allow the development of new effective treatments. Here, we aimed to characterize the specific transcriptomic response of white and brown adipose tissues after thermogenic induction. Using cold exposure to induce thermogenesis in mice, we identified mRNAs and miRNAs that were differentially expressed in several adipose depots. In addition, integration of transcriptomic data in regulatory networks of miRNAs and transcription factors allowed the identification of key nodes likely controlling metabolism and immune response. Moreover, we identified the putative role of the transcription factor PU.1 in the regulation of PPARγ-mediated thermogenic response of subcutaneous white adipose tissue. Therefore, the present study provides new insights into the molecular mechanisms that regulate non-shivering thermogenesis.https://doi.org/10.1038/s41598-023-33367-w |
| spellingShingle | Jordi Rodó Miquel Garcia Estefania Casana Sergio Muñoz Claudia Jambrina Victor Sacristan Sylvie Franckhauser Ignasi Grass Veronica Jimenez Fatima Bosch Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue Scientific Reports |
| title | Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue |
| title_full | Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue |
| title_fullStr | Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue |
| title_full_unstemmed | Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue |
| title_short | Integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue |
| title_sort | integrated gene expression profiles reveal a transcriptomic network underlying the thermogenic response in adipose tissue |
| url | https://doi.org/10.1038/s41598-023-33367-w |
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