Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
Ophioviruses (genus <i>Ophiovirus</i>, family <i>Aspiviridae</i>) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus <i>Ophiovirus</i> have a segmented single-stranded negative-sense RNA genome (ca. 11.3–1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/4/840 |
| _version_ | 1797603259479752704 |
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| author | Humberto Debat Maria Laura Garcia Nicolas Bejerman |
| author_facet | Humberto Debat Maria Laura Garcia Nicolas Bejerman |
| author_sort | Humberto Debat |
| collection | DOAJ |
| description | Ophioviruses (genus <i>Ophiovirus</i>, family <i>Aspiviridae</i>) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus <i>Ophiovirus</i> have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus <i>Ophiovirus</i> includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several <i>Asteraceae</i>, <i>Orchidaceae</i> and <i>Poaceae</i> crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. |
| first_indexed | 2024-03-11T04:27:41Z |
| format | Article |
| id | doaj.art-fefb1058de5948cf99f21942589e87fa |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T04:27:41Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fefb1058de5948cf99f21942589e87fa2023-11-17T21:44:31ZengMDPI AGViruses1999-49152023-03-0115484010.3390/v15040840Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics DataHumberto Debat0Maria Laura Garcia1Nicolas Bejerman2Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Camino 60 Cuadras Km 5,5, Córdoba X5020ICA, ArgentinaInstituto de Biotecnología y Biología Molecular (IBBM-CONICET-UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 50 y 115, La Plata 1900, ArgentinaInstituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Camino 60 Cuadras Km 5,5, Córdoba X5020ICA, ArgentinaOphioviruses (genus <i>Ophiovirus</i>, family <i>Aspiviridae</i>) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus <i>Ophiovirus</i> have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus <i>Ophiovirus</i> includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several <i>Asteraceae</i>, <i>Orchidaceae</i> and <i>Poaceae</i> crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus.https://www.mdpi.com/1999-4915/15/4/840plant virusesophiovirusvirus taxonomymetatranscriptomicsvirus discovery |
| spellingShingle | Humberto Debat Maria Laura Garcia Nicolas Bejerman Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data Viruses plant viruses ophiovirus virus taxonomy metatranscriptomics virus discovery |
| title | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_full | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_fullStr | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_full_unstemmed | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_short | Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data |
| title_sort | expanding the repertoire of the plant infecting ophioviruses through metatranscriptomics data |
| topic | plant viruses ophiovirus virus taxonomy metatranscriptomics virus discovery |
| url | https://www.mdpi.com/1999-4915/15/4/840 |
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