Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications
Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/11/1245 |
| _version_ | 1797549219750346752 |
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| author | Jayeshbhai Chaudhari Hiep L. X. Vu |
| author_facet | Jayeshbhai Chaudhari Hiep L. X. Vu |
| author_sort | Jayeshbhai Chaudhari |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first reverse genetics system for PRRSV was reported in 1998. Since then, several infectious cDNA clones for PRRSV have been constructed. The availability of these infectious cDNA clones has facilitated the genetic modifications of the viral genome at precise locations. Common approaches to manipulate the viral genome include site-directed mutagenesis, deletion of viral genes or gene fragments, insertion of foreign genes, and swapping genes between PRRSV strains or between PRRSV and other members of the <i>Arteriviridae</i> family. In this review, we describe the approaches to construct an infectious cDNA for PRRSV and the ten major applications of these infectious clones to study virus biology and virus–host interaction, and to design a new generation of vaccines with improved levels of safety and efficacy. |
| first_indexed | 2024-03-10T15:11:32Z |
| format | Article |
| id | doaj.art-fefc1fb91cfd40cda42c4a9076281b79 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T15:11:32Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-fefc1fb91cfd40cda42c4a9076281b792023-11-20T19:20:53ZengMDPI AGViruses1999-49152020-10-011211124510.3390/v12111245Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major ApplicationsJayeshbhai Chaudhari0Hiep L. X. Vu1Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USASchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USAPorcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first reverse genetics system for PRRSV was reported in 1998. Since then, several infectious cDNA clones for PRRSV have been constructed. The availability of these infectious cDNA clones has facilitated the genetic modifications of the viral genome at precise locations. Common approaches to manipulate the viral genome include site-directed mutagenesis, deletion of viral genes or gene fragments, insertion of foreign genes, and swapping genes between PRRSV strains or between PRRSV and other members of the <i>Arteriviridae</i> family. In this review, we describe the approaches to construct an infectious cDNA for PRRSV and the ten major applications of these infectious clones to study virus biology and virus–host interaction, and to design a new generation of vaccines with improved levels of safety and efficacy.https://www.mdpi.com/1999-4915/12/11/1245PRRSVreverse geneticsswine viruses |
| spellingShingle | Jayeshbhai Chaudhari Hiep L. X. Vu Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications Viruses PRRSV reverse genetics swine viruses |
| title | Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications |
| title_full | Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications |
| title_fullStr | Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications |
| title_full_unstemmed | Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications |
| title_short | Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications |
| title_sort | porcine reproductive and respiratory syndrome virus reverse genetics and the major applications |
| topic | PRRSV reverse genetics swine viruses |
| url | https://www.mdpi.com/1999-4915/12/11/1245 |
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